FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490673

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490673
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences539868
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC29550.5473560203605325No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTAGGAAGTAATGATTAACTAGA21450.39731934472871144No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC19990.37027569702223506No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18140.3360080612297821No Hit
CTCTATGGAAGGCAGGTGTATTATATAAGAGAGAAACAACACACAGCGCC17890.3313772996362074No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG17030.3154474797543103No Hit
ATATAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC14900.27599339097705367No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAACCACCGTG13790.25543280950158187No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13000.24079960286588573No Hit
GTTGTTCAGACTTGGCCCCCAATACCATATCATCCATATAACTGAAAGCC12800.23709499359102598No Hit
GCGCTGAACATGGAGAACATCACATCAGGATTCCTAGGACCCCTGCTCGT12510.23172331014247927No Hit
GTTTTGTACAGCAACAGGAGGGATACATAGAGGTTCCTTGAGCAGGAGTT11610.21505256840561024No Hit
CAGTCATAGAATATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCA11430.2117184200582365No Hit
ACCGCAGAGTCTAGACTCGTGGTGGACTTCTCTCAGTTTTCTAGGGGGAA11200.20745811939214773No Hit
CTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAA11040.20449443197225992No Hit
GTGTTTCCCCCTAGAAAACTGAGAGAAGTCCACCACGAGTCTAGACTCTT10280.19041691672779273No Hit
CTACAAACCTTGCCAGCAAATCCGCCTCCTGCCTCTACCAATCGCCAGTC10180.18856461209036282No Hit
GTACAAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC10140.18782369023539086No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT9990.18504523327924602No Hit
ATATAAGAGAGAAACAACACACAGCGCCTCATTTTGTGGGTCACCATATT9950.1843043114242741No Hit
GGGGAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAAT9600.17782124519326947No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9530.17652463194706855No Hit
GTTCCGCGCAGGGTCCCCAATCCTCGAGAAGATTGACGATATGGGAGAGA9520.17633940148332555No Hit
CCCTTATCCAATGGCAAATATTTGGTAACCTTTGGATAGAACCTAGCAGG9320.17263479220846578No Hit
GGATAAGGGTATTAAACCTTATTACCCAGAACATCTAGTTAATCATTACT8930.1654108041224892No Hit
ATACCATATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8920.1652255736587462No Hit
TCTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAGTACG8320.15411174583416687No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC8240.15262990212422295No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT7960.14744344913941926No Hit
AGTCTAGACTCTTCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC7730.1431831484733305No Hit
GACCTGCTGCGAGCAAAACAAGCTGCTAGGAGTTCCGCAGTATGGATCGG7690.14244222661835856No Hit
CTATAACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACAAT7600.14077515244467165No Hit
CTCTTATATAATACACCTGCCTTCCATAGAGTGTGTAAATAATGTCTGGT7540.13966376966221372No Hit
GCCTAGCAGCCATGGATACGATGTATATTTGCGGGAGAGAACAACAGAGT7470.1383671564160128No Hit
CCTTCGTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGG7440.13781146502478384No Hit
CATTTCTTGATAATGTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGT7390.1368853127060689No Hit
GTCCCGTGCTGGTAGTCGAAGATCCTGGAATTAGAGGACAAACGGGCAAC7380.1367000822423259No Hit
TCACCATACTGCTCTCAGGCAAGCAATTCTGTGCTGGGGGGACTTAATGA7210.13355116435869507No Hit
GCCTTAGAGTCTCCTGAACATTGTTCACCTCACCATACTGCTCTCAGGCA7170.13281024250372314No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCACTGAGGGTTAAAGA7080.13114316833003622No Hit
GTCCAAGAGTCCTCTTATATAAGACCTTGGGCAAGAATTGGTGGGCGTTC6960.12892040276512035No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA6820.1263271762727185No Hit
GCACGGGACCATGCAGAACCTGCACAACTCCTGCTCAAGGAACCTCTATG6770.12540102395400357No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT6710.12428964117154563No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6610.12243733653411575No Hit
ATATAATACACCTGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTAGGA6610.12243733653411575No Hit
TCTAGATACCGCCCAAGCTCTGTACCGGGAAGCCTTAGAGTCTCCTGAAC6600.12225210607037275No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA6500.12039980143294286No Hit
GTATTATATAAGAGAGAAACAACACACAGCGCCTCATTTTGTGGGTCACC6490.12021457096919987No Hit
GCTTTAGGGCATGGACATCGACCCTTATAAAGAATTTGGAGCTTCTGTGG6470.1198441100417139No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA6460.11965887957797092No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCGACTACC6370.11799180540428401No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATGTTGGATCTTC6370.11799180540428401No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6310.11688042262182607No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC6290.11650996169434011No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6180.11447242659316723No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC6140.11373150473819527No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTCTGCATGGTCCCGTG6120.11336104381070929No Hit
AAACAAAGGACGTCCCGCGCAGGATCCAGTTGGCAGCACAGCCTAGCAGC6120.11336104381070929No Hit
GGTCTATGCCAAGTGTTTGCTGACGCAACCCCCACTGGCTGGGGCTTGGT6100.11299058288322332No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT6090.11280535241948032No Hit
CTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5910.1094712040721065No Hit
GGCAATGACCCATAACATCCAATGACATAGCCCATGAAATGTAAAGAGTA5880.10891551268087755No Hit
TCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGTCAATGAGAAAAGA5770.10687797757970467No Hit
ATCCAAAGGTTACCAAATATTTGCCATTGGATAAGGGTATTAAACCTTAT5730.10613705572473271No Hit
ACAACACACAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAAA5710.10576659479724675No Hit
CTGTCTCTCCCATATCGTCAATCTTCTCGAGGATTGGGGACCCTGCGCGG5690.10539613386976075No Hit
ACACTCATCCTCAGGCCATGCAGTGGAACTCCACAACCTTCCACCAAACT5650.1046552120147888No Hit
GGTTAGGAAGTAATGATTAACTAGATGTTCTGGGTAATAAGGTTTAATAC5540.10261767691361592No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA5530.10243244644987293No Hit
CTTCTAGATACCGCCCAAGCTCTGTACCGGGAAGCCTTAGAGTCTCCTGA5490.10169152459490098No Hit
TCTTTTGTCTTTGGGTATACATTTAAACCCTAACAAAACAAAAAGATGGG5480.10150629413115798No Hit
ATGTATATTTGCGGGAGAGAACAACAGAGTTATCCGTCCCGAGAATGTTT5440.10076537227618604No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)