FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228471
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC19660.8605030835423313No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA17090.7480161596001244No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG12830.5615592350889171No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACATAGCGCC12580.5506169273124379No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10930.47839769598767456No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10260.44907231114671003No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACCTTCCACCAAACTCTGCAAG7390.32345461787272783No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT5820.25473692503643786No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA5490.24029307877148523No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5470.23941769414936687No Hit
CCCTTATCCAATGGCAAATATTTGGTGACATTTGGATAAAACCTAGCAGG5390.2359161556608935No Hit
GTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC4990.21840846321852667No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA4930.2157823093521716No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAATACG4920.21534461704111243No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTC4830.21140538624157987No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4800.21009230930840236No Hit
CTACAAACCTTGCCAGCAAATCCACCTCCTGCTTCTACCAATCGCCAGTC4790.2096546169973432No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT4770.2087792323752249No Hit
ATATAAGAGAGAAACTACACATAGCGCCTCATTTTGTGGGTCACCATATT4770.2087792323752249No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAGAAGGCCTCCGT4730.2070284631309882No Hit
CACATTTCTTGTCTCACTTTTGGAAGAGAAACGGTCATAGAATATTTGGT4640.20308923233145565No Hit
AGAACTGTGAAACCACAATAGTTGCCTGAACTTTAGGCCCATATTAGTGT4540.19871230922086391No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT4450.19477307842133137No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4310.18864538606650297No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC4260.18645692451120713No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT4180.18295538602273373No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4180.18295538602273373No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA4120.1803292321563787No Hit
CCCCAGAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAAAGCTCCAG4070.17814077060108285No Hit
GTATGGATCGGCAGAGGAGCCCGAAAGGTTCCACGCATGCGCTGATGGCC4060.17770307829002369No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAAT3930.17201307824625445No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATGA3930.17201307824625445No Hit
CATTTACACCAAGACATTATCAAAAGATGTGAACAATTTGTAGGCCCACT3920.17157538593519528No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG3890.17026230900201778No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC3860.16894923206884024No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC3840.1680738474467219No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3750.16413461664718937No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTTTACATTTCAT3620.15844461660342013No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT3530.1545053858038876No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA3530.1545053858038876No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG3520.1540676934928284No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC3460.1514415396264734No Hit
TTATATAAGAGAGAAACTACACATAGCGCCTCATTTTGTGGGTCACCATA3440.15056615500435502No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT3430.15012846269329588No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT3320.14531384727164498No Hit
AAACTACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAA3290.14400077033846745No Hit
CTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT3280.1435630780274083No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC3280.1435630780274083No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA3270.1431253857163491No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA3260.14268769340528994No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT3240.1418123087831716No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT3220.14093692416105325No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG3210.1404992318499941No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC3190.13962384722787574No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC3180.13918615491681657No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA3170.1387484626057574No Hit
GGGTTACTCTTTACATTTCATGGGTTATGTCATTGGATGTTATGGGTCAT3170.1387484626057574No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG3150.13787307798363907No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT3140.13743538567257987No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC3140.13743538567257987No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA3130.13699769336152073No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG3080.13480923180622487No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT3060.1339338471841065No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG3060.1339338471841065No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT3060.1339338471841065No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC3030.132620770250929No Hit
GAGTAACCCCATCTCTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA3000.1313076933177515No Hit
ACTACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA2960.1295569240735148No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT2960.1295569240735148No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG2960.1295569240735148No Hit
ATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC2930.1282438471403373No Hit
GTGTAGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA2900.1269307702071598No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC2870.12561769327398226No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGAAACGG2870.12561769327398226No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGC2840.12430461634080474No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTGACATTTGGATAAAAC2800.12255384709656805No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC2750.1203653855412722No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGAGTTTCTGT2730.11949000091915386No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC2720.11905230860809468No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG2710.1186146162970355No Hit
GAATACAGGTGCAATTTCCGTCCGAAGGTTTGGTACAGCAACAGGAGGGA2710.1186146162970355No Hit
GTCCTAAGCGGCCCCGAGAAGGGTCGTCCGCGGGATTCAGCGCCGACGGG2680.117301539363858No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT2670.11686384705279881No Hit
CATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC2670.11686384705279881No Hit
CATCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAAT2670.11686384705279881No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA2660.11642615474173966No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT2650.11598846243068048No Hit
GGGCAGAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCA2630.11511307780856213No Hit
CTTCTACTGTTCCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCT2620.11467538549750297No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG2620.11467538549750297No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA2580.11292461625326629No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCT2520.11029846238691124No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA2510.10986077007585208No Hit
GTCTTGGTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT2510.10986077007585208No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA2510.10986077007585208No Hit
TCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGAGC2450.10723461620949705No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA2450.10723461620949705No Hit
CTCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGG2450.10723461620949705No Hit
CACCAAGACATTATCAAAAGATGTGAACAATTTGTAGGCCCACTCACAGT2440.10679692389843787No Hit
CTCTGGGATCTTGCAGAGTTTGGTGGAAGGTTGTGGAATTCCACTGCATG2440.10679692389843787No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC2440.10679692389843787No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC2430.10635923158737871No Hit
GAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA2430.10635923158737871No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2420.10592153927631953No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG2330.10198230847678698No Hit
GTCTCCACCTTTGAGAAACACTCATCCTCAGGCCATGCAGTGGAATTCCA2320.10154461616572782No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA2300.10066923154360947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)