FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490675

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490675
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228471
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC17520.7668369289756687No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA14920.653036928100284No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG12290.5379238502917219No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACTACACATAGCGCC11280.49371692687474555No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA10460.4578261573678935No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9500.415807695506213No Hit
TCCTCAGGCCATGCAGTGGAATTCCACAACCTTCCACCAAACTCTGCAAG6970.3050715408082426No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT5950.2604269250802071No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5580.24423230957101777No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5410.23679154028301186No Hit
CCCTTATCCAATGGCAAATATTTGGTGACATTTGGATAAAACCTAGCAGG4990.21840846321852667No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA4790.2096546169973432No Hit
CACATTTCTTGTCTCACTTTTGGAAGAGAAACGGTCATAGAATATTTGGT4640.20308923233145565No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT4610.20177615539827815No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCTGACTTCTTTCCTTCAATACG4550.19915000153192308No Hit
GTCCCGTGCTGGTAGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC4450.19477307842133137No Hit
CTACAAACCTTGCCAGCAAATCCACCTCCTGCTTCTACCAATCGCCAGTC4330.1895207706886213No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4260.18645692451120713No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAGAAGGCCTCCGT4220.18470615526697043No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATATTCTATGA4160.1820800014006154No Hit
GCCTAGTCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4130.1807669244674379No Hit
AGAACTGTGAAACCACAATAGTTGCCTGAACTTTAGGCCCATATTAGTGT4080.17857846291214202No Hit
CATTTACACCAAGACATTATCAAAAGATGTGAACAATTTGTAGGCCCACT3990.17463923211260948No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG3990.17463923211260948No Hit
CCCCAGAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAAAGCTCCAG3880.16982461669095858No Hit
AGTCTGTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT3860.16894923206884024No Hit
GCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTAGGCTGCCT3860.16894923206884024No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAATTGGTGGGCGTTC3800.1663230782024852No Hit
ATATAAGAGAGAAACTACACATAGCGCCTCATTTTGTGGGTCACCATATT3580.15669384735918346No Hit
GTATGGATCGGCAGAGGAGCCCGAAAGGTTCCACGCATGCGCTGATGGCC3570.15625615504812426No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC3560.1558184627370651No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG3530.1545053858038876No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA3480.15231692424859172No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA3430.15012846269329588No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTGACATTTGGATAAAAC3390.14837769344905916No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT3290.14400077033846745No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT3270.1431253857163491No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA3270.1431253857163491No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACTACC3260.14268769340528994No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG3260.14268769340528994No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATATTGGATCTTC3230.14137461647211244No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT3230.14137461647211244No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC3220.14093692416105325No Hit
CATTTAAACCCTAACAAAACAAAGAGATGGGGTTACTCTTTACATTTCAT3210.1404992318499941No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA3210.1404992318499941No Hit
TTATATAAGAGAGAAACTACACATAGCGCCTCATTTTGTGGGTCACCATA3190.13962384722787574No Hit
GAGTAACCCCATCTCTTTGTTTTGTTAGGGTTTAAATGTATACCCAAAGA3110.13612230873940237No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG3010.13174538562881066No Hit
AAACTACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAA3000.1313076933177515No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT2970.12999461638457396No Hit
GTCCTAAGCGGCCCCGAGAAGGGTCGTCCGCGGGATTCAGCGCCGACGGG2950.12911923176245563No Hit
CTTCTAGATACCGCCTCAGCTCTATATCGGGAAGCCTTAGAGTCTCCTGA2940.12868153945139646No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT2940.12868153945139646No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCATCTTGAGTCCCTTTTTACC2900.1269307702071598No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC2880.12605538558504142No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAAATACAGGCCTCT2870.12561769327398226No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG2860.1251800009629231No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC2830.12386692402974557No Hit
ACTACACATAGCGCCTCATTTTGTGGGTCACCATATTCTTGGGAACAAGA2820.12342923171868639No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC2810.12299153940762723No Hit
CTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT2800.12255384709656805No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA2750.1203653855412722No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA2740.11992769323021302No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC2740.11992769323021302No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC2710.1186146162970355No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA2690.11773923167491716No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGAGTTTCTGT2680.117301539363858No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT2660.11642615474173966No Hit
GCTTAGGACTCTCTCGTCCCCTTCTCCGTCTGCCGTTTCGACCGACCACG2650.11598846243068048No Hit
GGCTTTCACTTTCTCGCCAACTTACAAGGCCTTTCTGTGTAAACAATACC2650.11598846243068048No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA2650.11598846243068048No Hit
TCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACTG2640.11555077011962131No Hit
CCTTATGAGTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCT2630.11511307780856213No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG2620.11467538549750297No Hit
ATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGACTCTA2580.11292461625326629No Hit
GTACCAAACCTTCGGACGGAAATTGCACCTGTATTCCCATCCCATCATCC2580.11292461625326629No Hit
GTGTAGTTTCTCTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAA2580.11292461625326629No Hit
GGGTTACTCTTTACATTTCATGGGTTATGTCATTGGATGTTATGGGTCAT2570.1124869239422071No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG2530.11073615469797042No Hit
GACCTGGCCGTTGCCGGGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC2530.11073615469797042No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT2520.11029846238691124No Hit
ATCCCAGAGTGAGAGGCCTGTATTTCCCTGCTGGTGGCTCCAGTTCAGGA2510.10986077007585208No Hit
CCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAAGAGTCCTCTTATGT2500.1094230777647929No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT2430.10635923158737871No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTTGGCAAGAAT2410.10548384696526036No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC2400.10504615465420118No Hit
CATCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAAT2400.10504615465420118No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATTCAATCTAAGC2390.10460846234314203No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG2380.10417077003208285No Hit
TCCTAGTACAAAGACCTTTAATCTAATCTCCTCCCCCAACTCCTCCCAGT2370.10373307772102366No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN2370.10373307772102366No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG2370.10373307772102366No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG2360.10329538540996451No Hit
GAACAAATGGCACTAGTAAACTGAGCCAAGAGAAACGGGCTGAGGCCCAC2350.10285769309890533No Hit
GGTCTGGAGCACACATTCTCGGGACGGATAACTCTGTTGTTCTCTCCCGC2350.10285769309890533No Hit
CTCCGAAAGACACCAAATATTCTATGACCGTTTCTCTTCCAAAAGTGAGA2310.10110692385466864No Hit
CTCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCAGTATGG2310.10110692385466864No Hit
GGGCAGAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCA2300.10066923154360947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)