FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490677

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490677
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences416895
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA21760.5219539692248648No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC18950.4545509061034553No Hit
CCTCCAGCTTATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA15860.38043152352510823No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG13960.33485649863874595No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT12010.28808213099221625No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGGGAAACAACACGTAGCGCC10750.2578586934359971No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC10750.2578586934359971No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10050.2410678947936531No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA9080.217800645246405No Hit
CTCTATGACTGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA8930.21420261696590268No Hit
ATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTCACGGTGGTCTCCATGC8810.21132419434150085No Hit
CTGTAATGTCAACGACCGACCTTGAGGCCTACTTCAAAGACTGTTTGTTT8190.19645234411542473No Hit
CTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGCACC8000.19189484162678852No Hit
CCCTTATCTAATGGCAAATATTTAGTAACATTTGGATAAAACCTAGCAGG7650.18349944230561652No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATT7510.18014128257714773No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTAGATGTTGGATCTTC7170.1719857518080092No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCTAGGCAACGGCCAGGTC7050.16910732918360738No Hit
CTTTATGAGTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCT7040.1688674606315739No Hit
TTTTATGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGGCAGA7010.16814785497547344No Hit
GTCTTTGAAGTAGGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA6910.1657491694551386No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT6870.16478969524700465No Hit
GTTTAATACCCTTATCTAATGGCAAATATTTAGTAACATTTGGATAAAAC6820.1635903524868372No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT6810.16335048393480373No Hit
CATTTAAACCCTAACAAAACTAAAAGATGGGGTTACTCTTTACATTTCAT6730.16143153551853584No Hit
CATCATACAGAAAATCAAAGAATGTTTTAGGAAACTTCCTACCAACAGGC6710.16095179841446886No Hit
AGTCTGTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTT6640.15927271855023448No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAGTGGTGGGCGTTC6600.15831324434210053No Hit
CTGTCTGATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGC6320.1515969248851629No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG6210.1489583708127946No Hit
GTATGGATCGGCAGAGGAGCCGGAAAGGTTCCACGCATGCGCTGATGGCC6200.1487185022607611No Hit
GTCCCGCGCTGGTGGTTGAAGATCCTGGAATTAGAGGACACACGGGCAAC6060.1453603425322923No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA6040.14488060542822534No Hit
GCCTTAGAGTCTCCTGAGCATTGTACACCTCACCATACTGCACTCAGGCA6000.1439211312200914No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACAAAACCTTCGGACGGAAATTGC5950.14272178845992398No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAG5950.14272178845992398No Hit
GAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5930.142242051355857No Hit
GAATACAGGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA5900.14152244569975653No Hit
CCTTTACCCCGTTGCTAGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG5890.14128257714772305No Hit
ACTGTGGAGTTACTCTCATTTTTGCCTTCTGACTTCTTTCCTTCGGTACG5890.14128257714772305No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5860.14056297149162258No Hit
GTACAGTAGAGGAATAAAGCCCCGTAAAGTTACCCACTTTATGAGTCCAA5760.13816428597128771No Hit
ATCAAGGTATGTTGCCCGTGTGTCCTCTAATTCCAGGATCTTCAACCACC5640.13528586334688592No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCCGGAT5620.13480612624281893No Hit
CATCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT5610.13456625769078545No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5590.13408652058671847No Hit
GTATCGGGATGCCTTAGAGTCTCCTGAGCATTGTACACCTCACCATACTG5530.13264730927451757No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5480.13144796651435015No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5480.13144796651435015No Hit
CAATAGGCCTGTTGGTAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT5470.13120809796231664No Hit
GTCTTGATGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGT5460.13096822941028316No Hit
TTTTGTGGGTCACCATATTCTTGGGAACAAGAGCTACAGCATGGGGCAGA5460.13096822941028316No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5410.12976888665011574No Hit
TCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGGAACTAATGA5330.12784993823384785No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT5320.12761006968181438No Hit
GGGTTTACTGTTCCTGAACTGGAGCCACCAGCAGGGAGATACAGGCCTCT5320.12761006968181438No Hit
CATTTACATCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT5310.1273702011297809No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA5270.12641072692164695No Hit
GTTCAGCGCAAGGTCCCCAATCCTCGAGAAGATTGACGATATGCGAGAGG5210.12497151560944603No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCTAAT5210.12497151560944603No Hit
GTAGAGGAATAAAGCCCCGTAAAGTTACCCACTTTATGAGTCCAAGGAAT5180.12425190995334556No Hit
GTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGCTTATAGAC5140.12329243574521163No Hit
GTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACTTCCAAACC5130.12305256719317814No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT5130.12305256719317814No Hit
CCTGTATTCAATCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5100.12233296153707769No Hit
GTTTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCTTCTCGGGGCCG5100.12233296153707769No Hit
CTATAAGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTC5100.12233296153707769No Hit
CAACAGAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCA5100.12233296153707769No Hit
AGATAAGGGTATTAAACCTTATTATCCGGAACATTTAGTTAATCATTACT5080.12185322443301072No Hit
CCCCAAAAGGCCTCCGTGCGGTGGGGTGAATCCCAGCCCGAATGCTCCAG5080.12185322443301072No Hit
GTCCAAGGAATACTAACATTGAGGTTCCCGAGATTGAGATCTTCTGCGAC5070.12161335588097721No Hit
CAGTAAACCCTGTTCCGACTACTGCCTCTCGCATATCGTCAATCTTCTCG5000.11993427601674284No Hit
CTTCTAGATACCGCCTCAGCTCTGTATCGGGATGCCTTAGAGTCTCCTGA4950.11873493325657539No Hit
GGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGCAG4940.11849506470454191No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA4930.11825519615250842No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4890.11729572194437447No Hit
GTACAGCACCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4880.117055853392341No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTAGGACATG4870.11681598484030752No Hit
TTGTACACCTCACCATACTGCACTCAGGCAAGCAATTCTTTGCTGGGGGG4840.11609637918420705No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4830.11585651063217357No Hit
AATATACATCGTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTG4790.11489703642403963No Hit
GGTTTGCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT4780.11465716787200614No Hit
GGGTTACTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCAT4680.11225848235167128No Hit
CTTTAGGACATGGACATCGACCCTTATAAAGAATTTGGAGCTACTGTGGA4670.1120186137996378No Hit
TCTCGGGGCCGCTTGGGGATCTTTCGTCCCCTTCTCCGTCTGCCGTTTCG4660.11177874524760431No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAGCACCTTGAGTCCCTTTTTACC4610.11057940248743689No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTGGGTCTTTTGGGTTTT4600.11033953393540341No Hit
CCTGTATTCCCATCCCATCATCCTGGGCTTTCGGAAAATTCCTATGGGAG4600.11033953393540341No Hit
GACCTGGCCGTTGCCTAGCAACGGGGTAAAGGTTCAGGTATTGTTTACAC4590.11009966538336992No Hit
CCCTGGGATCTTGCAGAGTTTGGTGGAAAGTTTTGGAGTTCCACTGCGCC4560.10938005972726947No Hit
ACTCTGTTGTTCTCTCCCGCAAATATACATCGTTTCCATGGCTGCTAGGC4550.10914019117523596No Hit
GCTTTAATGCCCTTGTATGCCTGTATTCAATCTAAACAGGCTTTCACTTT4550.10914019117523596No Hit
CTCTTTACATTTCATGGGCTATGTCATTGGATGTTATGGGTCATTGCCAC4510.10818071696710202No Hit
TTGCAAACCTTGCCAGCAAATCCGACTCCTGCATCTACCAATCGCCAGTC4470.10722124275896808No Hit
ATGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA4450.1067415056549011No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA4430.10626176855083413No Hit
ACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCCGCG4420.10602189999880066No Hit
TGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTT4410.10578203144676716No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC4340.10410295158253277No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGACCG4320.1036232144784658No Hit
GATACATAGAGGTTCCTTGAGCAGTAGTCGTGCAGGTTCTGCATGGTCCC4310.10338334592643232No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTGTGTCCTCTAATTCCAGGATC4260.10218400316626489No Hit
TAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACG4250.10194413461423141No Hit
CAGTTATATGGATGATGTGGTATTGGGGGCCAAGTCTGTACAGCACCTTG4210.10098466040609747No Hit
CTATTGTGGTTTCACATTTCTTGTCTCACTTTTGGAAGAGAAACAGTCAT4210.10098466040609747No Hit
ATGTGGTTATCCTGCTTTAATGCCCTTGTATGCCTGTATTCAATCTAAAC4200.10074479185406397No Hit
GAGTTATCCGTCCCGAGAATGTTTGCTCCAGACCTGCTGCGAGCAAAACA4190.1005049233020305No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)