FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490679

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490679
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences579696
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGA24780.4274654301564958No Hit
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA20240.3491485192238691No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACATAGCGCC18230.3144751731942259No Hit
CTCTATGACTGTGTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA17080.2946371891474152No Hit
GTCATAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC16710.28825453341061524No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAATACAAAGACC14990.25858380944495046No Hit
CGGTAGTTCCCCCTAGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTC13410.23132814440672353No Hit
GTATCTCACTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA13190.2275330518064641No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCTACAATACGTTGACAGACT13150.22684303497005326No Hit
ATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATATT12290.2120076729872209No Hit
GAACCACCGTGTGTCTTGGCCAAAATTCGCAGTCCCCAACCTCCAATCAC11510.19855234467721014No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC11490.19820733625900475No Hit
CTACTGTACCTGTCTTTAACCCTCATTGGAAAACACCCTCTTTTCCGAAT11350.19579227733156687No Hit
CCCTTATCCAATGGAAAATATTTGGTAACATTTGGATAAAACCTAGCAGG11050.19061715105848584No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACT9620.16594904915679942No Hit
GTATAATGTGATCTTGTGGCAATGACCCATAACATCCAATGACATAGCCC9360.16146393972012918No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT8870.15301123347409679No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT8750.15094118296486433No Hit
GTTTAATACCCTTATCCAATGGAAAATATTTGGTAACATTTGGATAAAAC8530.1471460903646049No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACCACCGTG8460.14593856090088597No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT8380.14455852722806437No Hit
ATATTAGTGTTGACATAACTGACTACTAGGTCCCTGGATACCGGATCCTC8280.14283348513703734No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA8250.14231597250972924No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT8140.14041842620959952No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8040.1386933841185725No Hit
TTATATAAGAGAGAAACAACACATAGCGCCTCATTTTGTGGGTCACCATA8020.1383483757003671No Hit
GAATACTAACAGTGGGAGTCCCGAGATTGAGATCTTCTGCGACGCGGCGA8000.1380033672821617No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7930.13679583781844276No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTATGA7890.13610582098203197No Hit
GTATTAAACCTTATTATCCAGAACATCTAGTTAATCATTACTTCCAAACC7820.13489829151831306No Hit
GTATTTCCCTGCTGGTGGCTCCAGTTCAGGAACAGTAAACCCTGTTCCGA7800.13455328310010764No Hit
CTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCA7760.13386326626369682No Hit
GAATACATGCATACAAGGGCATTAAAGCAGGATAACCACATTGTGTAAAA7740.13351825784549143No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG7720.13317324942728603No Hit
CTTATAAGGCCTTTCTGTGTAAACAATACCTGAACCTTTACCCCGTTGCC7670.13231072838177252No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT7540.1300681736634374No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC7220.12454803897215092No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCTACAAT7170.12368551792663741No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCCTTGTATGCATGTATT7040.12144296320830228No Hit
CGGTATTTGCTCTGAAGGCTGGATCCAACTGGTGGTCGGGAAAGAATCCC7030.12127045899919958No Hit
CATTATACAGAAAATCAAAGAATGTTTTAGAAAACTTCCTGTTAACAGGC6960.12006292953548067No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT6860.11833788744445364No Hit
GTTTTCCTGGTTGACAAGAATCCTCACAATACCGCAGAGTCTAGACTCGT6850.11816538323535095No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG6750.11644034114432392No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6750.11644034114432392No Hit
CCTTATGAGTCCAAGGAATACTAACAGTGGGAGTCCCGAGATTGAGATCT6730.11609533272611851No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACCGTTTGTTT6710.11575032430791311No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTCTATTC6640.1145427948441942No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGCCTTATAAGTTGGCGA6630.1143702906350915No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAGATGTTCTGGAT6520.11247274433496177No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG6470.11161022328944827No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGAAAATATTTGGTAACATT6430.11092020645303746No Hit
CTATGAATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC6370.10988518119842124No Hit
GCAGTGAGATACAGGCCTCGCACTCTGGGATCTTGTAGAGTTTGGTGGAA6360.10971267698931854No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA6240.1076426264800861No Hit
CATTTGGATAAAACCTAGCAGGCATAATTAATTGCAGTCTTCTTTTCTCA6220.1072976180618807No Hit
CTTCTAGATACCGCCTCAGCTCTGTTTCGGGAAGCTTTAGAGTCTCCTGA6160.1062625928072645No Hit
GCAAACACTGCAAATCCAGATTGGGACTTCAATCCCAACAAGGACACCTG6160.1062625928072645No Hit
GGCCTATTGATTGGAAAGTCTGTCAACGTATTGTAGGTCTTTTGGGTTTT6100.1052275675526483No Hit
GTCAAGAAGGCAGCCGACCCCGCTGTCTCCACCTTTGAGAGACACTCATC6070.10471005492534016No Hit
CAGCTGTAAAAAGGGACTCAAGATGCTGTACAGACTTGGCCCCCAATACC6030.10402003808892936No Hit
GTGTAAATGTATATTCGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5990.10333002125251856No Hit
ATCCAAATGTTACCAAATATTTTCCATTGGATAAGGGTATTAAACCTTAT5990.10333002125251856No Hit
GGCTTTCACTTTCTCGCCAACTTATAAGGCCTTTCTGTGTAAACAATACC5980.10315751704341586No Hit
GTGAGATACAGGCCTCGCACTCTGGGATCTTGTAGAGTTTGGTGGAAGGT5960.10281250862521046No Hit
CAATAGGCCTGTTAACAGGAAGTTTTCTAAAACATTCTTTGATTTTCTGT5920.10212249178879966No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCCCGCATGCGCTGATGGCC5880.10143247495238883No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACCAGGAAAACCCC5820.10039744969777263No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5810.10022494548866992No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)