FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490682

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490682
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358641
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17170.47875173223362644No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA14780.41211127562102495No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC14740.41099595417144164No Hit
GGATACTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTA13760.3836705786566511No Hit
ATTTCGGGAAGCCTTGGAGTCTCCTGAGCATTGTACACCTCACCATACTG12500.3485379529947775No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10480.29221421979082146No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAAAACAAACACC10240.2855222910933217No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG10140.2827339874693635No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT9000.25094732615623977No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC8770.2445342278211359No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT8630.24063060274759437No Hit
ATTTAGGGAAGCCTTGGAGTCTCCTGAGCATTGTACACCTCACCATACTG8580.2392364509356153No Hit
GTCCTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC8330.23226569187571972No Hit
TCCAACATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGCC8140.2269679149901991No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7310.2038249949113459No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA7300.20354616454895005No Hit
CCAGTATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGCCT7280.20298850382415842No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT7060.19685423585145034No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6760.18848932497957566No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT6600.18402803918124253No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA6560.18291271773165924No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT6520.18179739628207595No Hit
GGATGTTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTA6510.18151856591968013No Hit
GCACTAAGGCATGTAATTCTTTGCTGGGGGGACTTAATGACTCTAGCCAC6290.17538429794697205No Hit
GGCTTTCACTTTCTCGCCAACTTACAAAACCTTTCTGTGTAAACAATACC6280.1751054675845762No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC6230.1737113157725971No Hit
CTACAAACCTTGCCAGCAAATCCACCTCCTGCCTCTACCAATCGCCAGTC6150.17148067287343052No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC6020.16785587816228484No Hit
TCCCTAAATAGAGCTGCGGCGGTATCTAGAAGATCTCGTACTGAAGGAAA5970.16646172635030573No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTAGGA5970.16646172635030573No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5910.16478874417593078No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT5860.1633945923639517No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG5800.16172161018957676No Hit
GGTTCAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAA5780.1611639494647851No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5710.15921213692801436No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5610.15642383330405615No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5590.1558661725792645No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG5560.15502968149207705No Hit
CCCACAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG5430.15140488678093134No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5420.15112605641853552No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5330.14861658315697313No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5330.14861658315697313No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5320.1483377527945773No Hit
CTCTAGGACCGTTTCTTTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5300.14778009206978565No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC5290.14750126170738984No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATTTAGTTAATCATTACT5130.14303997590905668No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT5090.1419246544594734No Hit
CATGCATACAATGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC5040.14053050264749428No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT4960.1382998597483277No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT4950.13802102938593191No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA4890.13634804721155697No Hit
CATTTGAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT4840.13495389539957786No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA4780.1332809132252029No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG4600.12826196670207812No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4550.12686781489009902No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4550.12686781489009902No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGAAT4520.12603132380291154No Hit
TCCAACATCCAGGGAACTAGTAGTCAGTTATGTTAACACTAATATGGGCC4480.12491600235332825No Hit
GAATACTAACAGTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4420.12324302017895333No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC4390.12240652909176586No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT4340.12101237727978675No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG4340.12101237727978675No Hit
GCATAGCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTTGGC4310.12017588619259928No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCATTGTATGCATGTATT4300.11989705583020345No Hit
GGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGCCTAAAGTTCAGG4280.1193393951054118No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT4260.11878173438062017No Hit
TCACCATACTGCACTAAGGCATGTAATTCTTTGCTGGGGGGACTTAATGA4250.11850290401822436No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGTTTTGTAAGTTGGCGA4230.11794524329343271No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCCGACTTCTTTCCTTCAGTACG4060.11320512713270373No Hit
GTTAGTATTCCTTGGACTCATAAGGTGGGAAACTTTACGGGGCTTTATTC3970.11069565387114134No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT3960.11041682350874552No Hit
GATTTCGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG3960.11041682350874552No Hit
ATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCATG3930.10958033242155804No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC3890.10846501097197475No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG3890.10846501097197475No Hit
CCATATTCTTGGGAACAAGATCTACAGCATGGGGCAGAATCTTTCCACCA3790.10567670734801654No Hit
GTATTAAACCTTATTATCCAGAACATTTAGTTAATCATTACTTCCAAACC3770.1051190466232249No Hit
AGTCGGAGGACAGGAGGTTGGTGAGTGATTGGAGGTTGGGGACTGCGAAT3750.10456138589843327No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA3720.10372489481124579No Hit
GAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAATACTAAC3720.10372489481124579No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC3700.10316723408645415No Hit
GATACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCC3690.10288840372405832No Hit
TTATTTACACACTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAA3680.1026095733616625No Hit
GATCTACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGATTCT3680.1026095733616625No Hit
CCTTATGAGTCCAAGGAATACTAACAGTGAGATTCCCGAGATTGAGATCT3650.10177308227447503No Hit
GCTTTAGGGCATGGACATTGATCCTTATAAAGAATTTGGAGCTACTGTGG3640.10149425191207921No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATACTGGATCTTC3600.10037893046249594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)