FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490683

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490683
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences358641
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCAGCTTATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA17750.494923893252584No Hit
TCCATAGAGTGTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAA14490.40402519511154605No Hit
CTCTATGGAAGGCGGGTATATTATATAAGAGAGAAACAACACGTAGCGCC13650.38060344467029705No Hit
ATTTCGGGAAGCCTTGGAGTCTCCTGAGCATTGTACACCTCACCATACTG13140.3663830961881101No Hit
GGATACTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTA12730.3549510513298814No Hit
GTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAATACGTTGACAGACT10010.27910919275821783No Hit
GTGCTGGTGCGCAGACCAATTTATGCCTACAGCCTCCTAAAACAAACACC9900.27604205877186383No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACTACCGTG9810.2735325855103014No Hit
GTCCTAGAGTATTTGGTGTCTTTCGGAGTGTGGATTCGCACTCCTCCAGC8940.24927434398186488No Hit
AGTCTAGACTCTGCGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC8900.24815902253228161No Hit
ATTTAGGGAAGCCTTGGAGTCTCCTGAGCATTGTACACCTCACCATACTG8620.24035177238519856No Hit
ATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATATT7850.21888183448072027No Hit
CCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAACCTAGCAGG7330.20438265563613753No Hit
AGCTGGAGGAGTGCGAATCCACACTCCGAAAGACACCAAATACTCTAGGA6730.1876528338923882No Hit
GTACCGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC6720.1873740035299924No Hit
GGATGTTGGATCTTCCAAATTACCACCCACCCAGGTGGCTAGAGTCATTA6570.18319154809405505No Hit
TCCAACATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGCC6430.17928792302051355No Hit
CTGTAATGTCAACGACCGACCTTGAGGCATACTTCAAAGACTGTTTGTTT6340.17677844975895116No Hit
ATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTCACGGTGGTCTCCATGC6270.1748266372221804No Hit
GCACTAAGGCATGTAATTCTTTGCTGGGGGGACTTAATGACTCTAGCCAC6190.17259599432301384No Hit
CCAGTATCCAGGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGCCT6180.172317163960618No Hit
GGTTTGTAGTATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGGCCTCCGT6160.17175950323582637No Hit
TTATATAAGAGAGAAACAACACGTAGCGCCTCATTTTGTGGGTCACCATA6070.16925002997426394No Hit
CATTTAAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT6060.16897119961186816No Hit
CATTTACACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACT6000.1672982174374932No Hit
GTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATTTGGATAAAAC5960.16618289598790992No Hit
GGATAACCACATTGTGTAAAAGGGGCAGCAAAACCCAAAAGACCCACAAT5930.16534640490072247No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTACAGAGAGTCCAA5880.16395225308874334No Hit
GGCTTTCACTTTCTCGCCAACTTACAAAACCTTTCTGTGTAAACAATACC5850.16311576200155586No Hit
GTGTAAATGTATATTAGGAAAAGAGGGTGTTTTCCAATGAGGGTTAAAGA5670.15809681547843107No Hit
CTCTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGT5550.1547508511296812No Hit
GTATGCATGTATTCAATCTAAGCAGGCTTTCACTTTCTCGCCAACTTACA5550.1547508511296812No Hit
CTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGCACC5550.1547508511296812No Hit
CTTATATAATATACCCGCCTTCCATAGAGTGTGTAAATAATGTCTGGTTT5430.15140488678093134No Hit
CTACAAACCTTGCCAGCAAATCCACCTCCTGCCTCTACCAATCGCCAGTC5340.14889541351936897No Hit
GGTTCAAATGTATACCCAGAGACAAAAGAAAATTGGTAACAGCGGTAAAA5340.14889541351936897No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN5310.1480589224321815No Hit
CTCTAGGACCGTTTCTTTTCCAAAAGTGAGACAAGAAATGTGAAACCACA5290.14750126170738984No Hit
GTCCCGTGCTGGTGGTTGAAGATCCTGGAATTAGAGGACAAACGGGCAAC5270.1469436009825982No Hit
CACTAATATGGGCCTAAAGTTCAGGCAACTATTGTGGTTTCACATTTCTT5120.14276114554666086No Hit
GGATAAGGGTATTAAACCTTATTATCCAGAACATTTAGTTAATCATTACT4960.1382998597483277No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCTTCAACCACC4930.13746336866114023No Hit
GATTTAGATCTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGA4900.13662687757395278No Hit
CATTTGAACCCTAACAAAACAAAAAGATGGGGTTACTCTTTACATTTCAT4900.13662687757395278No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAATTGGTGGGCGTTC4850.13523272576197368No Hit
ACATAGAGGTTCCTTGAGCAGGAGTCGTGCAGGTTCTGCATGGTCCCGTG4720.131607931050828No Hit
TCCCTAAATAGAGCTGCGGCGGTATCTAGAAGATCTCGTACTGAAGGAAA4700.13105027032603633No Hit
GAATCTTTCCACCAGCAATCCTCTGGGATTCTTTCCCGACCACCAGTTGG4520.12603132380291154No Hit
CAATAGGCCTGTTAACAGGAAGTTTCCTAAAACATTCTTTGATTTTCTGT4520.12603132380291154No Hit
CAGTAAACCCTGTTCCGACTACTGTCTCTCACATATCGTCAATCTTCTCG4440.12380068090374496No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4420.12324302017895333No Hit
CTTTTACACAATGTGGTTATCCTGCTTTAATGCCATTGTATGCATGTATT4410.12296418981655749No Hit
GTACAGCATCTTGAGTCCCTTTTTACCGCTGTTACCAATTTTCTTTTGTC4400.12268535945416169No Hit
GAATACTAACAGTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA4390.12240652909176586No Hit
GCACGGGACCATGCAGAACCTGCACGACTCCTGCTCAAGGAACCTCTATG4340.12101237727978675No Hit
ATGCTGTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAG4330.12073354691739092No Hit
CCTCTATGTATCCCTCCTGTTGCTGTACCAAACCTTCGGACGGAAATTGC4270.119060564743016No Hit
CATGCATACAATGGCATTAAAGCAGGATAACCACATTGTGTAAAAGGGGC4270.119060564743016No Hit
GAACAAATGGCACTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCAC4240.11822407365582853No Hit
ACTGTGGAGTTACTCTCGTTTTTGCCTTCCGACTTCTTTCCTTCAGTACG4150.11571460039426612No Hit
GCCTAGCAGCCATGGAAACGATGTATATTTGCGGGAGAGAACAACAGAGT4110.11459927894468284No Hit
GTATTAAACCTTATTATCCAGAACATTTAGTTAATCATTACTTCCAAACC4100.11432044858228703No Hit
GGGACCTAGTAGTCAGTTATGTTAACACTAATATGGGCCTAAAGTTCAGG4070.11348395749509955No Hit
CCTTTACCCCGTTGCCCGGCAACGGCCAGGTCTGTGCCAAGTGTTTGCTG4050.11292629677030791No Hit
GGGTAAAGGTTCAGGTATTGTTTACACAGAAAGGTTTTGTAAGTTGGCGA4040.11264746640791208No Hit
GCATAGCCTGAGGATGAGTGTTTCTCAAAGGTGGAGACAGAGGGGTTGGC4000.1115321449583288No Hit
ACATTACAGAGAGTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGAAT3990.11125331459593299No Hit
GTGTAAACAATACCTGAACCTTTACCCCGTTGCCCGGCAACGGCCAGGTC3940.10985916278395387No Hit
CTCTAGGACCGTTTCTCTTCCAAAAGTGAGACAAGAAATGTGAAACCACA3930.10958033242155804No Hit
GTGTAAATAATGTCTGGTTTGGAAGTAATGATTAACTAAATGTTCTGGAT3910.1090226716967664No Hit
GTATGGATCGGCAGAGGAGCCTGAAAGGTTCCACGCATGCGCTGATGGCC3860.1076285198847873No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC3850.10734968952239148No Hit
TCCTCAGGCTATGCAGTGGAACTCCACAACCTTCCACCAAACTCTGCAAG3780.10539787698562071No Hit
GATCTACAGCATGGGGCAGAATCTTTCCACCAGCAATCCTCTGGGATTCT3750.10456138589843327No Hit
GTAGAAGAATAAAGCCCCGTAAAGTTTCCCACCTTATGAGTCCAAGGAAT3750.10456138589843327No Hit
CCCACAGACAAAAGAAAATTGGTAACAGCGGTAAAAAGGGACTCAAGATG3720.10372489481124579No Hit
TCACCATACTGCACTAAGGCATGTAATTCTTTGCTGGGGGGACTTAATGA3650.10177308227447503No Hit
GACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAACAGTG3650.10177308227447503No Hit
GCCTAATCATCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC3620.10093659118728757No Hit
CCTTATGAGTCCAAGGAATACTAACAGTGAGATTCCCGAGATTGAGATCT3610.10065776082489174No Hit
GGATAATAAGGTTTAATACCCTTATCCAATGGCAAATATTTGGTAACATT3610.10065776082489174No Hit
ATATTAGTGTTAACATAACTGACTACTAGGTCCCTGGATACTGGATCTTC3600.10037893046249594No Hit
CACCAAGACATTATCAAAAAATGTGAACAATTTGTAGGCCCACTCACAGT3600.10037893046249594No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)