FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490686

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490686
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444743
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC21890.4921943684330052No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT21420.4816264674205103No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA20920.47038401953487746No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT18500.4159705717684145No Hit
CTTACAAGGCCTTTCTAAGTAAACAGTATCTGAACCTTTACCCCGTTGCT16470.3703262333527453No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA13410.3015224522926724No Hit
CTTATAAGGCCTTTCTAAGTAAACAGTATCTGAACCTTTACCCCGTTGCT12530.2817357440139586No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA12380.27836300964826877No Hit
GTATAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT11680.2626235826083828No Hit
GTACCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA11640.2617241867775322No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA11580.2603750930312562No Hit
GTTTTATACAGCAACATGAGGGAAACATAGAGGTTCCTTGAGCAGGAGTT10970.2466593066107842No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG10700.2405883847525425No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT10390.23361806706345012No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA10360.23294352019031217No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT9620.21630469731957558No Hit
TCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAG9400.21135802024989714No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG9360.2104586244190465No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT8880.199665874448839No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG8710.19584344216772384No Hit
GACTTCTTTCCTTCTATTCGAGATCTCCTCGACACCGCCTCTGCTCTGTA8640.19426949946373523No Hit
GTTGTGAGTAGGCCCTGAGCCTGTGGGCTCCACCCCAACAGTCCCCCATG8600.19337010363288462No Hit
CCTTATAAGTTGGCGAGAAAGTAAAAGCCTGTTTTGCTTGTATACATGCA8600.19337010363288462No Hit
ATATAAAAGAGAGTCCACACGTAGCGCTTCATTTTGCGGGTCACCATATT8580.19292040571745928No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA8200.18437614532437835No Hit
ATCGACGACTGGGGACCCTGTACCGAACATGGAGAACATCGCATCAGGAC7950.17875492138156193No Hit
CTTCAGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGAGGGAGTTCT7920.17808037450842396No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG7520.1690864161999177No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA7520.1690864161999177No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7490.16841186932677973No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT7450.16751247349592913No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG7240.16279064538396332No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG7140.16054215580683676No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG7140.16054215580683676No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC7100.15964275997598612No Hit
ATGGCAGAGACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCC7100.15964275997598612No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT7100.15964275997598612No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA6800.15289729124460644No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC6790.15267244228689378No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC6780.15244759332918112No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA6750.15177304645604314No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6740.15154819749833048No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG6730.15132334854061785No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6720.15109849958290517No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6690.15042395270976722No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6680.15019910375205456No Hit
GTCTTCGATAAGATTGACGATATGGCAGAGACAGTATTCTGAGCAGGGCT6640.14929970792120392No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6590.14817546313264066No Hit
ATTCAAGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGA6520.14660152042865207No Hit
AGTTCATCATTACTTCCAGACGCGACATTATTTACACACTCTTTGGAAGG6510.14637667147093938No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6430.14457787980923814No Hit
GAGTTTTGCCCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCG6400.14390333293610016No Hit
GTTTTGTACAGCAACATGAGGGAAACATAGAGGTTCCTTGAGCAGGAGTT6370.1432287860629622No Hit
CATGTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT6370.1432287860629622No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6270.14098029648583563No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6250.14053059857041034No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA6230.14008090065498502No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT6150.13828210899328378No Hit
GGTTTTATACAGCAACATGAGGGAAACATAGAGGTTCCTTGAGCAGGAGT6050.1360336194161572No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5980.1344596767121686No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5950.13378512983903063No Hit
CAATTATTGTGGTTCCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGT5860.13176148921961672No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5840.1313117913041914No Hit
GCCTTATAAGTTGGCGAGAAAGTAAAAGCCTGTTTTGCTTGTATACATGC5780.12996269755791548No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5740.12906330172706484No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5740.12906330172706484No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCT5710.12838875485392687No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5630.12658996319222562No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5630.12658996319222562No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5630.12658996319222562No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA5610.1261402652768003No Hit
TGATTAACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGG5590.12569056736137502No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5500.12366692674196109No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5470.12299237986882312No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5470.12299237986882312No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG5470.12299237986882312No Hit
AACGTGCAACATACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGC5440.12231783299568515No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC5420.12186813508025984No Hit
CTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGAGGGAGTTCT5390.12119358820712187No Hit
GCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT5390.12119358820712187No Hit
TCGCAGAAGATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACA5360.1205190413339839No Hit
CTCTCCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAACA5060.11377357260260419No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA5040.1133238746871789No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5040.1133238746871789No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG5030.11309902572946623No Hit
CAAGATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGG5000.11242447885632825No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA4970.11174993198319028No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4940.11107538511005233No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4920.11062568719462701No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG4910.11040083823691436No Hit
GTAGCTCTTGTTCCCAAGAATATGGTGACCCGCAAAATGAAGCGCTACGT4900.11017598927920169No Hit
TCCTAGTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACT4870.10950144240606374No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG4860.10927659344835107No Hit
GGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCTTTTGGGGTTT4850.10905174449063841No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG4810.10815234865978779No Hit
GCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTTGCTTGTATACATGC4780.10747780178664981No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCCA4750.10680325491351185No Hit
GTTTTGCCCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCGCA4740.10657840595579919No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACAA4670.1050044632518106No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4640.10432991637867263No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT4620.10388021846324731No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGTC4610.10365536950553465No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC4580.10298082263239669No Hit
CAGTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT4570.10275597367468403No Hit
GGTTCCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCTGGAATAT4500.10118203097069543No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4470.10050748409755747No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA4450.10005778618213214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)