FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490687

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490687
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences444743
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC21390.4809519205473723No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA21070.4737567539005673No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT20410.458916722691532No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT17480.3930359780817236No Hit
CTTACAAGGCCTTTCTAAGTAAACAGTATCTGAACCTTTACCCCGTTGCT14010.3150133897554318No Hit
CCTCCTGCATATAGACCACAAAATGCCCCTATCTTATCAACACTTCCGGA12900.2900551554493269No Hit
CTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGGA12130.2727417857054523No Hit
GTATAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT12120.2725169367477397No Hit
CTTATAAGGCCTTTCTAAGTAAACAGTATCTGAACCTTTACCCCGTTGCT11820.26577146801636004No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG11090.24935749410333607No Hit
GTACCTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA11020.2477835513993475No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10810.24306172328738168No Hit
GTTTTATACAGCAACATGAGGGAAACATAGAGGTTCCTTGAGCAGGAGTT10430.23451746289430078No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTATG9440.21225741608074775No Hit
AAGATGCAGGTCCCCTAGAAGAAGAACTCCCTCGCCTCGCAGACGAAGGT9430.2120325671230351No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA9410.2115828692076098No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT9320.20955922858819587No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT9210.20708589005335667No Hit
TCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCCAG9180.20641134318021867No Hit
ATATAAAAGAGAGTCCACACGTAGCGCTTCATTTTGCGGGTCACCATATT8750.19674283799857445No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG8680.19516889529458586No Hit
CTTCAGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGAGGGAGTTCT8510.1913464630134707No Hit
CCTTATAAGTTGGCGAGAAAGTAAAAGCCTGTTTTGCTTGTATACATGCA8420.1893228223940568No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT8120.1825773536626771No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG7850.17650643180443537No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7790.17515733805815942No Hit
ATCGACGACTGGGGACCCTGTACCGAACATGGAGAACATCGCATCAGGAC7600.17088520786161895No Hit
GTTGTGAGTAGGCCCTGAGCCTGTGGGCTCCACCCCAACAGTCCCCCATG7580.17043550994619364No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC7570.17021066098848098No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG7450.16751247349592913No Hit
GACTTCTTTCCTTCTATTCGAGATCTCCTCGACACCGCCTCTGCTCTGTA7390.16616337974965317No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT7370.16571368183422786No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA7230.16256579642625066No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC7170.1612167026799747No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG6970.1567197235257216No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC6910.15537062977944566No Hit
GAGTTTTGCCCCAGACCTGCTGCGAGCAAAACAAGCGGCTAGGAGTTCCG6840.15379668707545707No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA6840.15379668707545707No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6780.15244759332918112No Hit
AGTTCATCATTACTTCCAGACGCGACATTATTTACACACTCTTTGGAAGG6760.15199789541375583No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6710.15087365062519253No Hit
ATGGCAGAGACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCC6590.14817546313264066No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA6560.14750091625950268No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6440.1448027287669508No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6420.14435303085152548No Hit
GCCTTATAAGTTGGCGAGAAAGTAAAAGCCTGTTTTGCTTGTATACATGC6400.14390333293610016No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC6360.14300393710524956No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6310.14187969231668626No Hit
GTCTTCGATAAGATTGACGATATGGCAGAGACAGTATTCTGAGCAGGGCT6270.14098029648583563No Hit
GCTTTAGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG6270.14098029648583563No Hit
GTTTTGTACAGCAACATGAGGGAAACATAGAGGTTCCTTGAGCAGGAGTT6210.1396312027395597No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT6190.13918150482413438No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA6130.13783241107785843No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG6080.13670816628929516No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT6020.13535907254301924No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC5990.13468452566988126No Hit
CTTCTGCGACGCGGCGATTGAGACCTTCGTCTGCGAGGCGAGGGAGTTCT5990.13468452566988126No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5980.1344596767121686No Hit
TGATTAACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGG5950.13378512983903063No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCT5950.13378512983903063No Hit
GCTTTGGGGCATGGACATTGACCCGTATAAAGAATTTGGAGCTTCTGTGG5920.13311058296589265No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT5920.13311058296589265No Hit
ATTCAAGATCTCCTCGACACCGCCTCTGCTCTGTATCGGGAGGCCTTAGA5780.12996269755791548No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5720.12861360381163953No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA5700.12816390589621424No Hit
CATGTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT5650.12703966110765094No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5610.1261402652768003No Hit
CAATTATTGTGGTTCCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGT5570.1252408694459497No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA5490.12344207778424844No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5470.12299237986882312No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT5440.12231783299568515No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA5410.12164328612254717No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5300.11916994758770795No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG5260.11827055175685733No Hit
GGTTTTATACAGCAACATGAGGGAAACATAGAGGTTCCTTGAGCAGGAGT5220.1173711559260067No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5210.11714630696829403No Hit
GCCTTGTAAGTTGGCGAGAAAGTAAAAGCCTGTTTTGCTTGTATACATGC5190.11669660905286874No Hit
AACGTGCAACATACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGC5170.11624691113744341No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC5130.11534751530659279No Hit
ACATAGAGGTTCCTTGAGCAGGAGTTGTGCAGGTTTTGCATGGTCCGGTG5130.11534751530659279No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG5070.11399842156031685No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC5060.11377357260260419No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC5030.11309902572946623No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5030.11309902572946623No Hit
CCAAAAGACACCAAATATTCCAGAACAGTTTCTCTCCCAAAAGTAAGACA5000.11242447885632825No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA4880.10972629136377639No Hit
GGTTCCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCTGGAATAT4860.10927659344835107No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT4840.10882689553292577No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC4800.10792749970207514No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG4780.10747780178664981No Hit
GCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT4770.10725295282893717No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCCA4760.1070281038712245No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGAACAA4740.10657840595579919No Hit
GGTTTTGGGGGCCAAGTCTGTACAACATCTTGAGTCCCTTTATGCCGCTG4700.10567901012494857No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTG4700.10567901012494857No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA4670.1050044632518106No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACGAGAAAAACCCC4670.1050044632518106No Hit
CTCTCCCATATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAACA4650.10455476533638527No Hit
CATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT4640.10432991637867263No Hit
TCGCAGAAGATCTCAATCTCGGGAATCTCAATGTTAGTATTCCTTGGACA4620.10388021846324731No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCCAGAA4610.10365536950553465No Hit
CAAGATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGG4550.10230627575925871No Hit
CTGCCTCTTCGTCTAACAACAGTAGTTTCCGGAAGTGTTGATAAGATAGG4530.10185657784383341No Hit
TCCTAGTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACT4460.10028263513984481No Hit
GCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTT4460.10028263513984481No Hit
CAATATTTGTCTGATTTTTAGGCCCATATTAACGTTGACATAGCTGACTA4460.10028263513984481No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT4450.10005778618213214No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)