FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490688

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490688
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515938
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC26590.5153720020622633No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG23390.4533490458155825No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA21370.41419705468486523No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC20280.3930704852133396No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT19510.378146211366482No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG17470.338606576759223No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC16240.31476650295190506No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA14570.2823982726606685No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG14420.27949094658660534No Hit
ATCTAAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTATGTGCCAAGTG14060.2725133640088538No Hit
ATATAAAAGAGAGTCCACCCGTAGCGCCTCATTTTGCGGGTCACCATATT13350.2587520205916215No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCGATTACATATCCCATGAA13050.2529373684434952No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG12560.24344010326822216No Hit
ATTAATAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACAAC12520.24266481631513864No Hit
GCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCCGTG11550.22386410770286352No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC11280.21863092076954985No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA11180.21669270338684105No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT11160.2163050599102993No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT10760.20855219037946418No Hit
GCCACATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAACATGGA10450.202543716493067No Hit
CTATTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCCAGGTG10400.20157460780171263No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT10340.20041167737208737No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT10320.2000240338955456No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA10310.1998302121572747No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG10260.19886110346592034No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACCCGTAGCGCC9960.193046451317794No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG9790.1897514817671891No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT9720.18839472959929293No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA9560.1852935817869589No Hit
GGTTTAGATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA9560.1852935817869589No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA9390.181998612236354No Hit
GCACAACTCCTGCTCAAGGAACCTCTTTGTTTCCCTCATGTTGCTGTACA9340.1810295035449996No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA9130.17695924704131116No Hit
GAATTAATAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA8990.17424574270551887No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG8840.17133841663145571No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC8790.17036930794010133No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC8760.1697878427252887No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA8760.1697878427252887No Hit
TTGAAGTCCCAATCTGGATTTTCTGAGTTGGCTTTGAATGCAGGGTCCAA8730.16920637751047607No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT8710.16881873403393433No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA8630.1672681601277673No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC8620.16707433838949642No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT8510.16494229926851675No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC8350.16184115145618272No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT8200.15893382538211956No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG8100.15699560799941079No Hit
GACTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCCAGG8090.1568017862611399No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA8060.15622032104632727No Hit
GTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC8040.15583267756978553No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC8010.15525121235497288No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTTTG7990.15486356887843114No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA7920.15350681671053498No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA7720.14963038194511744No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG7710.14943656020684654No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG7700.14924273846857566No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT7660.14846745151549218No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT7600.14730452108586692No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA7560.1465292341327834No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC7520.1457539471796999No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC7490.14517248196488725No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA7420.14381572979699112No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC7350.14245897762909496No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA7320.14187751241428234No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7270.14090840372292796No Hit
GGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTT7150.1385825428636774No Hit
CGATATGGCAGAGACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGG7130.13819489938713567No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA7070.13703196895751038No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC7070.13703196895751038No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG7010.13586903852788515No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA6970.13509375157480163No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG6960.13489992983653076No Hit
GAGTCGTGCAGGTTTTGCATGGTCCGGTGCTGGTTGTTGATGATCCTGGA6920.13412464288344725No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6890.1335431776686346No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG6830.13238024723900935No Hit
GGTATGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC6800.1317987820241967No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6790.13160496028592583No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCTGACTCTGGG6790.13160496028592583No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6750.13082967333284232No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG6660.12908527768840442No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6630.12850381247359177No Hit
GGAGTCGTGCAGGTTTTGCATGGTCCGGTGCTGGTTGTTGATGATCCTGG6630.12850381247359177No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT6610.12811616899705003No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC6600.12792234725877916No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATGTGGCAGAGACAGTA6550.12695323856742477No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT6550.12695323856742477No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT6540.12675941682915387No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT6470.12540266466125774No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG6450.12501502118471597No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6440.12482119944644511No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTC6360.12327062554027811No Hit
GAGTAAACAGTATCTAAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTA6310.12230151684892371No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA6300.12210769511065284No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6160.11939419077486055No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC6150.11920036903658966No Hit
GCTCAGAATACTGTCTCTGCCACATCGTCAATCTTATCGAAGACTGGGGA6150.11920036903658966No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA6080.11784361686869352No Hit
CCCTTAACTTCATGGGATATGTAATCGGGAGTTGGGGCACATTGCCACAG6070.11764979513042265No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT6000.1162930429625265No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA5930.11493629079463036No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA5930.11493629079463036No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTGCTATTCG5920.11474246905635947No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5880.11396718210327597No Hit
GACGGGGAGTCCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGTCGGTACAA5850.11338571688846333No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5850.11338571688846333No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5790.11222278645883808No Hit
TACCAGGCCGTTGCCGAGCAACGGGGTAAAGGTTTAGATACTGTTTACTC5770.11183514298229633No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTATGTGCCAAGTGTTTGCTG5710.11067221255267107No Hit
ATATAACTGAAAGCCAGACAGTGGGGGAAAGCCCTACGAACCACTGAACA5660.10970310386131668No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGAAGAGGC5640.10931546038477492No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC5580.10815252995514965No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAA5560.1077648864786079No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCACTCACC5550.10757106474033702No Hit
GGACCTCAACCCGCACAAGGACAACTGGCCGGACGCCAACAAGGTGGGAG5540.10737724300206614No Hit
CCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTTGGTCTTCCAAAC5510.1067957777872535No Hit
CTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCCAGGTG5500.10660195604898264No Hit
TTAATAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACAACT5420.1050513821428156No Hit
GGGTAAAGGTTTAGATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGA5410.10485756040454473No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG5410.10485756040454473No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGTC5360.10388845171319033No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5320.10311316476010683No Hit
TCCTAGTACAAAGACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACT5320.10311316476010683No Hit
GTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATG5280.10233787780702332No Hit
CTCCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGG5240.1015625908539398No Hit
GATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGGGGA5240.1015625908539398No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT5210.10098112563912719No Hit
ATAAAAGAGAGTCCACCCGTAGCGCCTCATTTTGCGGGTCACCATATTCT5190.10059348216258544No Hit
GGTCCTAGGAGTCCTGATGCGATGTTCTCCATGTTCGGTACAGGGTCCCC5160.1000120169477728No Hit
GCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCGTTGACATACT5160.1000120169477728No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT5160.1000120169477728No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)