FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490689

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490689
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences515938
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC23660.4585822327488962No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG22110.42853986331691013No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA18240.3535308506060806No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC18170.3521740984381844No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT17900.3469409115048707No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG15430.299066942151964No Hit
ATCTAAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTATGTGCCAAGTG13890.26921839445824886No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG13600.2635975640483934No Hit
ATATAAAAGAGAGTCCACCCGTAGCGCCTCATTTTGCGGGTCACCATATT13230.25642615973237093No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC13140.254681764087933No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA12120.23491194678430355No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG11220.21746799033992456No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA11010.21339773383623617No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC10940.21204098166833998No Hit
ATTAATAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACAAC10660.2066139729967554No Hit
GCCTAGCAGCCATGGAAAGGATGTATACTTGCGGGAGAGCACGACAGAAT10600.20545104256713015No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCGATTACATATCCCATGAA10410.20176842953998347No Hit
CTATTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCCAGGTG9650.1870379774313968No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT9620.18645651221658416No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9580.18568122526350064No Hit
GCTGAAAGTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCCGTG9200.17831599920920732No Hit
GAATTAGTAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA9170.17773453399439468No Hit
GCCACATCGTCAATCTTATCGAAGACTGGGGACCCTGTACCGAACATGGA9150.1773468905178529No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT9100.17637778182649852No Hit
GTATACATCCTTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC8960.17366427749070623No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT8920.17288899053762272No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA8800.17056312967837223No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACCCGTAGCGCC8760.1697878427252887No Hit
GGTTTAGATACTGTTTACTCAGAAAGGCCTTGTAAGTTGGCGAGAAAGTA8660.1678496253425799No Hit
GAATTAATAGTCAGCTATGTCAACGTTAATATGGGCCTAAAAATCAGACA8540.1655237644833294No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA8490.164554655791975No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA8380.16242261667099533No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8340.16164732971791185No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT8270.16029057755001572No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC8160.15815853842903604No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG8120.15738325147595253No Hit
GCACAACTCCTGCTCAAGGAACCTCTTTGTTTCCCTCATGTTGCTGTACA8080.15660796452286904No Hit
GACTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCCAGG7760.15040566889820095No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA7700.14924273846857566No Hit
TTGAAGTCCCAATCTGGATTTTCTGAGTTGGCTTTGAATGCAGGGTCCAA7680.14885509499203392No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC7620.14769216456240866No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA7610.1474983428241378No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG7560.1465292341327834No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT7500.14536630370315815No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT7460.14459101675007463No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA7440.14420337327353286No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC7410.14362190805872024No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG7410.14362190805872024No Hit
GCACCGGACCATGCAAAACCTGCACGACTCCTGCTCAAGGAACCTCTTTG7330.1420713341525532No Hit
ATGTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAAC7170.13897018634021915No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA6850.1327678907155511No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG6840.13257406897728022No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA6830.13238024723900935No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC6810.13199260376246758No Hit
GTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC6810.13199260376246758No Hit
GTACAACAGGCGGAGAAGCGGGCGGTAGAGCCCCAAGCGGCCCCGGGAGG6740.13063585159457144No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6730.13044202985630057No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA6690.12966674290321706No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA6630.12850381247359177No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC6590.12772852552050828No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT6520.12637177335261213No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6460.12520884292298687No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA6450.12501502118471597No Hit
GTTCCTGAACTGGAGCCACCAGCAGGAAAGTACAGGGCCCTGACTCTGGG6360.12327062554027811No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA6340.12288298206373635No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG6300.12210769511065284No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC6270.12152622989584021No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC6270.12152622989584021No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC6240.12094476468102756No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT6230.12075094294275669No Hit
CGATATGGCAGAGACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGG6230.12075094294275669No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG6210.12036329946621493No Hit
GAGTCGTGCAGGTTTTGCATGGTCCGGTGCTGGTTGTTGATGATCCTGGA6160.11939419077486055No Hit
GGATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTT6160.11939419077486055No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT6130.11881272556004792No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6120.11861890382177703No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTATGTGCCAAGTGTTTGCTG6110.11842508208350616No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG6100.11823126034523528No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6080.11784361686869352No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG6080.11784361686869352No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG6070.11764979513042265No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGTCCGCGTAAAGAGAGGTGC6030.11687450817733913No Hit
TATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAATTCG6020.11668068643906826No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5960.11551775600944299No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC5940.11513011253290123No Hit
GGTATGTGCCAAGTGTTTGCTGACGCAACCCCCACTGGTTGGGGCTTGGC5900.11435482557981771No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5880.11396718210327597No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5870.11377336036500509No Hit
GCTCAGAATACTGTCTCTGCCACATCGTCAATCTTATCGAAGACTGGGGA5830.11299807341192157No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5800.11241660819710897No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA5770.11183514298229633No Hit
CTACTAATTCCCTGGATGCTGGATCTTCCAAATTACTTCCCACCCAGGTG5770.11183514298229633No Hit
CCCTTAACTTCATGGGATATGTAATCGGGAGTTGGGGCACATTGCCACAG5760.11164132124402545No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT5730.11105985602921283No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5730.11105985602921283No Hit
GTCCAAGAGTCCTCTTATGCAAGACCTTGGGCAGGTTCCCGTGGGCGTTC5630.10912163864650404No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATGTGGCAGAGACAGTA5500.10660195604898264No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA5290.10253169954529419No Hit
GAATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA5280.10233787780702332No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5270.10214405606875243No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA5220.10117494737739804No Hit
GAGTAAACAGTATCTAAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTA5200.10078730390085631No Hit
CCTTATGTGTCCAAGGAATACTAACATTGAGATTCCCGAGATTGAGATCT5200.10078730390085631No Hit
CCCTTACAGTAAATGAAAACAGGAGACTAAAATTAATTATGCCTGCTAGG5200.10078730390085631No Hit
CTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACA5180.10039966042431456No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)