FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490691

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490691
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences491856
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA21600.4391529228066751No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC17370.35315214209036794No Hit
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA16420.3338375459484077No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN14580.2964282228945057No Hit
GTATAGCCCCAAGCGGCCCCGGGAGGGGTCGTCCGCGGGATTCAGCGCCG12510.254342734458866No Hit
ATATAAAAGAGAGTCCACACGTAGCGCCTCATTTTGCGGGTCACCATATT12440.25291955369051106No Hit
TCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG10410.21164731140821705No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA10260.2085976383331707No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA10070.2047347191047786No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC10060.20453140756644222No Hit
GGATAATACGGTTTGATTCCTTTATCTAAGGGCAAATATTTAGTAACATT9970.2027016037214144No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9910.20148173449139584No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG9900.20127842295305942No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG9390.19090953449790182No Hit
CCTTTTTTCATTTACTGTAAGGGGCCCCACAAATTGCTCACATCTATCAA9360.19029959988289255No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA9350.19009628834455614No Hit
GTATAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAAAACCACATC9320.18948635372954686No Hit
TCTCTTGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGCCTTGGGTGG9230.18765654988451905No Hit
GCACCGGACCATGCAAAACCTGCACAACTCCTGCTCAAGGAACCTCTTTG9050.18399694219446344No Hit
GTATTTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG9030.18359031911779058No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCTCGT8770.17830421912104355No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG8570.1742379883543151No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGAGACATTATT8540.17362805373930582No Hit
GTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGC8180.16630883835919455No Hit
CACAACAGCTTGCCTGAGTGCCGTATGGTGAGGTGAACAATGTTCCGGAG7960.16183598451579323No Hit
ACTACATACTCTGGATAATACGGTTTGATTCCTTTATCTAAGGGCAAATA7910.16081942682411113No Hit
GTATACATGCATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAA7800.15858299990241045No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT7670.15593994990403695No Hit
GTTTTGCTTGTATACATGCATATAAAGGCATTAAAGCAGGATATCCACAT7560.1537035229823363No Hit
GGGCTATACCGCCCGCTTCTCCGCCTATTGTACCGACCGACCACGGGGCG6990.14211476529716016No Hit
CCTAACCTCCTCCCCCAACTCCTCCCACTCAGTAAACACACAGTCTTTGA6860.13947171529878663No Hit
CCTATTGTACCGACCGACCACGGGGCGCACCTCTCTTTACGCGGACTCCC6840.1390650922221138No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT6770.13764191145375881No Hit
CCTTTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAA6570.13357568068703035No Hit
GTGGATATCCTGCTTTAATGCCTTTATATGCATGTATACAAGCAAAACAG6570.13357568068703035No Hit
GGAACATATTGTACAAAAAATCAAACTGTGTTTTAGGAAACTTCCTGTAA6550.1331690576103575No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6540.13296574607202108No Hit
TTATACCGCTGTTACCAATTTTCTTTTGTCTTTGGGTATACATTTAAACC6520.13255912299534822No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT6510.1323558114570118No Hit
TCCAAAGAGTGTGTAAATAATGTCTCGTCTGGAAGTAATGATTAACTACA6400.13011938453531116No Hit
GGAATTAGAGGACAAACGGGCAACATACCTTGATAGTCCAGAAGAACCAA6360.12930613838196545No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT6280.12767964607527407No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACAGTTTGATTTTTTGT6180.1256465306919098No Hit
CTGTAAGGGGCCCCACAAATTGCTCACATCTATCAACAATGTCCTCCTGC6090.12381672684688202No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC6060.12320679223187274No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG6060.12320679223187274No Hit
GTTTACAGGAAGTTTCCTAAAACACAGTTTGATTTTTTGTACAATATGTT5990.12178361146351778No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGCGCC5920.12036043069516282No Hit
ATGTAATTGGGAGTTGGGGCACATTGCCACAGGAACATATTGTACAAAAA5830.11853062685013499No Hit
TTCCCCCACTGTCTGGCTTTCAGTTATATGGATGATGTGGTTTTGGGGGC5790.1177173806967893No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT5780.11751406915845289No Hit
GTTCACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTC5750.1169041345434436No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5720.11629419992843434No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG5720.11629419992843434No Hit
GAAAAAAGGAGACTAAAATTAATTATGCCTGCTAGGTTTTATCCCAATGT5700.1158875768517615No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5690.11568426531342506No Hit
GTACAATAGGCGGAGAAGCGGGCGGTATAGCCCCAAGCGGCCCCGGGAGG5670.11527764223675221No Hit
GTATGTAGTTAATCATTACTTCCAGACGAGACATTATTTACACACTCTTT5660.1150743306984158No Hit
CCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAGTGTTTGCTG5570.11324452685338797No Hit
GTTCAAATACTGTTTACTTAAAAAGGCCTTGTAAGTTGGCGAGAAAGTAA5500.11182134608503302No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT5490.11161803454669658No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC5410.10999154224000521No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTTTAAGTAAACAGTATT5410.10999154224000521No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTATAA5350.10877167300998666No Hit
CATATAAAGGCATTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA5280.10734849224163169No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC5260.10694186916495885No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG5200.1057219999349403No Hit
GAATACAGGTGCAGTTTCCGTCCGTAGGTTTTGTACAGCAACATGAGGGA5200.1057219999349403No Hit
TAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTGCAGT5180.10531537685826746No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC5160.1049087537815946No Hit
GTACAGACTTGGCCCCCAAAACCACATCATCCATATAACTGAAAGCCAGA5090.10348557301323964No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5040.10246901532155751No Hit
CTCTACTTCCAGGATCATCAACAACCAGCACCGGACCATGCAAAACCTGC5030.10226570378322111No Hit
CATTAAGGTTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT5030.10226570378322111No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTTTA5000.10165576916821184No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)