FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490692

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490692
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences477752
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCTCCTGCATATAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA21460.4491870259046534No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAAAGTCCAA20780.43495369982752563No Hit
GTACAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT18740.3922537215961419No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAACACCCGTG15440.3231802273983154No Hit
ATATAAAAGAGAGTCCACACGTAGTGCCTCATTTTGCGGGTCACCATATT15090.31585425074097023No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCC14320.2997371020948107No Hit
ACTCACAACTGTGCCAGCAGCTCCTCCTCCTGCCTCCACCAATCGGCAGT13620.28508514878012026No Hit
GTTGTGAGTAGGCCCTGAGCCTGAGGGCTCCACCCCAACAGTCCCCCATG12360.2587116328136774No Hit
ATATGTTCCTGTGGCAATGTGCCCCAACTCCCAATTACATATCCCATGAA11930.2497111472060818No Hit
CTTTTATATAAGATCCCCGCCTTCCAAAGAGTGTGTAAATAATGTCGCGT11830.2476180110182689No Hit
GACCTTTAACCTAACCTCCTCCCCCAACTCCTCCCACTCATTAAACACAC11750.24594350206801857No Hit
GGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTAACATT11540.2415479160736114No Hit
GTATAAAACCTACGGACGGAAACTGCACCTGTATTCCCATCCCATCATCT10890.2279425308528274No Hit
TCCTCAGGCCATGCAGTGGAACTCCACCACTTTCCACCAAACTCTTCAAG10650.2229190040020764No Hit
CATTAAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGATTGAGACCT10600.22187243590816993No Hit
CCCTTATCTCCACCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAA10550.22082586781426347No Hit
GTCCGGTGCTGGTTGTTGATGATCCTGGAATTAGAGGACAAACGGGCAAC10360.2168489090574189No Hit
GCTCAGAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGA10100.2114067549691053No Hit
CATTTAAACCCTCACAAAACAAAAAGATGGGGATATTCCCTTAACTTCAT9960.2084763643061672No Hit
ACTACATACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATA9520.19926656507979038No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGG9230.1931964701351329No Hit
GTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGC9110.19068470670975737No Hit
GGTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAA8490.17770726234531722No Hit
CCACCAAATGCCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAG8450.17687000787019205No Hit
TCCAAAGAGTGTGTAAATAATGTCGCGTCTGGAAGTAATGATTAACTACA8400.17582343977628562No Hit
CTCTTTGGAAGGCGGGGATCTTATATAAAAGAGAGTCCACACGTAGTGCC8320.17414893082603528No Hit
ACCTATGGGAGTGGGCCTCAGTCCGTTTCTCTTGGCTCAGTTTACTAGTG8310.17393961720725395No Hit
GATCATTAACCTAACCTCCTCCCCCAACTCCTCCCACTCATTAAACACAC8280.1733116763509101No Hit
CATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATT8260.17289304911334752No Hit
ATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGGAAAGT8210.17184648101944106No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG8190.17142785378187847No Hit
CCCATAGGTATTTTGCGAAAGCCCAAGATGATGGGATGGGAATACAGGTG8180.17121854016309718No Hit
GTATTATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATT7970.16682295416869003No Hit
GTCTTCGATAAGATTGACGATATGGCAGAGACAGTATTCTGAGCAGGGCT7730.161799427317939No Hit
GTATACATGCATATAAAGGCAGTAAAGCAGGATATCCACATTGCGTGAAA7620.15949697751134478No Hit
CTCCAAAAGACACCAAATATTCAAGAACAGTTTCTCTCCCAAAAGTAAGA7520.15740384132353188No Hit
CCTCTAAGGGACACTCATCCTCAGGCCATGCAGTGGAACTCCACCACTTT7510.15719452770475056No Hit
GATGTATACTTGCGGGAGAGCACGACAGAATTGTCAGTCCCGATGAGTTT7440.15572933237328151No Hit
CTCATTAAACACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7370.1542641370418125No Hit
GGAACATATTGTACAAAAAATCAAAATGTGTTTTAGGAAACTTCCTGTAA7330.15342688256668732No Hit
GTGGTATTGTGAGGATTTTTGTCAACAAGAAAAACCCCGCCTGTAACACG7230.1513337463788744No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG7220.1511244327600931No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC7210.1509151191413118No Hit
ATATTAACGTTGACATAGCTGACTACTAATTCCCTGGATGCTGGATCTTC7160.14986855104740535No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATCATCAACAACC7150.14965923742862405No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT7150.14965923742862405No Hit
TGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAAGAA7130.14924061019106147No Hit
TATAAAGGCAGTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCAAA7030.14714747400324857No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG7020.14693816038446725No Hit
CTAGTAAACTGAGCCAAGAGAAACGGACTGAGGCCCACTCCCATAGGTAT7000.14651953314690466No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACGGCACTCAGGCA6900.14442639695909174No Hit
GCCTAGCAGCCATGGAAATGATGTATACTTGCGGGAGAGCACGACAGAAT6870.14379845610274786No Hit
CAATAGGCCTGTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGT6800.14233326077127884No Hit
ATTTTGTGGTCTATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACA6750.14128669267737234No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGCAGAGGA6700.1402401245834659No Hit
GTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACACACTCTTT6680.1398214973459033No Hit
GTACAGACTTGGTCCCCAAAACCACATCATCCATATAACTGAAAGCCAAA6660.13940287010834074No Hit
GTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAATTTGG6580.1377283611580904No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG6530.13668179306418393No Hit
CATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAACTTACAAGGCC6480.13563522497027747No Hit
TCTATCAACAATGTCCTCCTGCAAATGAATGTCAGGAAAAGAAGGAGTTT6470.13542591135149618No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA6420.1343793432575897No Hit
GTTTACAGGAAGTTTCCTAAAACACATTTTGATTTTTTGTACAATATGTT6420.1343793432575897No Hit
GCCTTTATATGCATGTATACAAGCAAAACAGGCTTTTACTTTCTCGCCAA6370.13333277516368325No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGCATAAA6280.1314489525946516No Hit
CGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCCT6240.13061169811952644No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCTGCATATAGACCACCAAATGCC6170.1291465027880574No Hit
CCCAATTACATATCCCATGAAGTTAAGGGAATATCCCCATCTTTTTGTTT6170.1291465027880574No Hit
GCCTAATCATCTCATGTTCATGTCCTACTGTTCAAGCCTCCAAGCTGTGC6080.1272626802190258No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGGAGAGAAACTGTTCT6050.1266347393626819No Hit
CTGTAAGGGGCCCCACAAATTGCTTACATCTATCAACAATGTCCTCCTGC6010.12579748488755674No Hit
ATATGCAGGAGGAGTGCGAATCCACACTCCAAAAGACACCAAATATTCAA5940.12433228955608767No Hit
GTATGGATCGGCAGAGGAGACACAAAGGTTCCACGCATGCGCTGATGGCC5920.12391366231852509No Hit
CCCCAACAGTCCCCCATGGGGAGGGGTGAACCCTGGCCCGAATGCTCCCA5900.12349503508096252No Hit
GTCCAACTGATGATCGGGGAAGAATCCCAGGGGATTGGGGACAGAAAGAT5820.12182052613071218No Hit
GTGGATATCCTGCTTTACTGCCTTTATATGCATGTATACAAGCAAAACAG5760.12056464441802442No Hit
ATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGG5740.12014601718046183No Hit
GAATACTGTCTCTGCCATATCGTCAATCTTATCGAAGACTGGGGACCCTG5710.11951807632411796No Hit
ACTCTGGATAATACGGTTTGATCCCTTTATCTAAGGGCAAATATTTAGTA5600.11721562651752374No Hit
GTGCTGGTGAACACACCAATTTATGCCTACAGCCTCCTAGTACAAAGACC5600.11721562651752374No Hit
TAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTT5530.11575043118605469No Hit
GGCTTTTACTTTCTCGCCAACTTACAAGGCCTTTCTAAGTAAACAGTATC5530.11575043118605469No Hit
GCATAAAGGGACTCAAGATGTTGTACAGACTTGGTCCCCAAAACCACATC5510.1153318039484921No Hit
CAGTTATATGGATGATGTGGTTTTGGGGACCAAGTCTGTACAACATCTTG5490.11491317671092952No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAA5490.11491317671092952No Hit
CAGTTGGACCCTGCATTCAAAGCCAACTCAGAAAATCCAGATTGGGACCT5490.11491317671092952No Hit
CCTGTAAACAGGCCTATTGATTGGAAAGTATGTCAACGAATTGTGGGTCT5480.11470386309214824No Hit
ATAAAAGAGAGTCCACACGTAGTGCCTCATTTTGCGGGTCACCATATTCT5470.11449454947336693No Hit
GTACAGGGTCCCCAGTCTTCGATAAGATTGACGATATGGCAGAGACAGTA5420.11344798137946047No Hit
GTATACATCATTTCCATGGCTGCTAGGCTGTGCTGCCAACTGGATCCTGC5410.11323866776067919No Hit
TCCTTGGACACATAAGGTGGGAAACTTTACGGGGCTTTATTCTTCTACGG5340.11177347242921014No Hit
GCCTGAGGATGAGTGTCCCTTAGAGGTGGAGATAAGGGAGTAGGCTGCCT5340.11177347242921014No Hit
GTATCTGAACCTTTACCCCGTTGCTCGGCAACGGCCTGGTCTGTGCCAAG5270.11030827709774109No Hit
GATGTAAGCAATTTGTGGGGCCCCTTACAGTAAATGAAACCAGGAGACTA5220.10926170900383463No Hit
CCTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAAATACCTATGGGAG5200.10884308176627204No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTAATTCCAGGATC5130.10737788643480298No Hit
GTTATATGGATGATGTGGTTTTGGGGACCAAGTCTGTACAACATCTTGAG5070.10612200472211525No Hit
CCATATTCTTGGGAACAAGATCTACAGCATGGGAGGTTGGTCTTCCAAAC5060.10591269110333396No Hit
TGGAAACCACCGTGAACGCCCACGGGAACCTGCCCAAGGTCTTGCATAAG5060.10591269110333396No Hit
GGATATCCACATTGCGTGAAAGGGGCGGCAAACCCCAAAAGACCCACAAT5050.10570337748455265No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGAAGAGGC5040.10549406386577136No Hit
GTCCACACGTAGTGCCTCATTTTGCGGGTCACCATATTCTTGGGAACAAG5000.1046568093906462No Hit
CCCTTAACTTCATGGGATATGTAATTGGGAGTTGGGGCACATTGCCACAG5000.1046568093906462No Hit
GTACTTTCCTGCTGGTGGCTCCAGTTCAGGAACAGTGAGCCCTGCTCAGA5000.1046568093906462No Hit
CATATAAAGGCAGTAAAGCAGGATATCCACATTGCGTGAAAGGGGCGGCA4990.1044474957718649No Hit
ATCTCAATGTTAGTATTCCTTGGACACATAAGGTGGGAAACTTTACGGGG4950.10361024129673974No Hit
CCCGTGTGTCTTGGCCAAAATTCGCAGTCCCAAATCTCCAGTCACTCACC4940.10340092767795843No Hit
GAGTCGTGCAGGTTTTGCATGGTCCGGTGCTGGTTGTTGATGATCCTGGA4910.10277298682161456No Hit
GCTCTGTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCA4910.10277298682161456No Hit
ATTCTGTGTTGGGGTGAGTTAATGAATCTAGCCACCTGGGTGGGAAGTAA4900.10256367320283326No Hit
ACAGTATTCTGAGCAGGGCTCACTGTTCCTGAACTGGAGCCACCAGCAGG4860.10172641872770809No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGTC4820.10088916425258293No Hit
ATCCAGAGTATGTAGTTAATCATTACTTCCAGACGCGACATTATTTACAC4800.10047053701502034No Hit
GTTTTATACAGCAACATGAGGGAAACATAGAGGTTCCTTGAGCAGGAGTC4800.10047053701502034No Hit
GATCTACAGCATGGGAGGTTGGTCTTCCAAACCTCGAAAAGGCATGGGGA4790.10026122339623904No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)