FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490694

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490694
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454260
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGGAGCGGCAAAGCCCAAAAGCCCCACAATTCGTTGACATACT20370.44842160877030773No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA19090.4202439131774755No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT18210.4008717474574032No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA15350.3379122088671686No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA14830.32646502003258043No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC14770.32514419055166643No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA13820.3042310571038612No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG13380.29454497424382514No Hit
CATTTAAACCCTGCTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT13050.28728041209879807No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC12670.278915158719676No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACC10630.23400695636859947No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCACG10570.23268612688768547No Hit
ATGTAAGACCTTGGGCAAGACCTGACGGGCGTTCACGGTGGTCTCCATGC10010.22035838506582134No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG9850.2168361731167173No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG9730.21419451415488924No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC9420.20737022850350018No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA9360.20604939902258618No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT9090.20010566635847313No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT9020.1985646986307401No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCT8680.19107999823889402No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8610.189539030511161No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG8540.18799806278342798No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG8490.18689737154933297No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG8200.18051336239158192No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA8090.17809184167657288No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACCC7720.16994672654426982No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA7560.16642451459516577No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG7540.16598423810152776No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG7080.15585787874785365No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT7010.15431691102012063No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA7000.15409677277330164No Hit
TCCACAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG6990.15387663452648262No Hit
GTGGTGGAGTGAACCCTGGCCCGAAGGATCCCACTCCTACCTGGTGTGCC6960.15321621978602562No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6950.15299608153920663No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC6860.1510148373178356No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6840.1505745608241976No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCATG6760.1488134548496456No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6570.14463082816008455No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC6560.14441068991326553No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6530.14375027517280853No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGACTAAAATTGATTATG6460.1422093074450755No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG6360.1400079249768855No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT6360.1400079249768855No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6320.13912737198960948No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT6280.13824681900233346No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT6190.13626557478096246No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAGTCTCCTTTTTTCG6180.13604543653414344No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC6150.13538502179368642No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG6090.13406419231277242No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAGTCTCCTTTTTTCGTTAAC6060.13340377757231542No Hit
CTCTTACAGTTAACGAAAAAAGGAGACTAAAATTGATTATGCCTGCTAGG5980.1316426715977634No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG5950.13098225685730638No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG5930.13054198036366838No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5900.12988156562321138No Hit
CTCCACCACATGGCAATCTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATA5870.12922115088275435No Hit
CAATTCTCTAGGGGGAGCTCCCACGTGTCCTGGCCAAAATTTGCAGTCCC5870.12922115088275435No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC5850.12878087438911637No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5840.12856073614229735No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5780.12723990666138335No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATTCCTATGGGAG5700.12547880068683134No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG5690.12525866244001232No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACCCGCAGCGCC5660.12459824769955531No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG5620.12371769471227932No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACCCGCAGCGCCTCAT5560.1223968652313653No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5560.1223968652313653No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGCAGGGTTTAAATGTATA5520.12151631224408929No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA5470.12041562100999427No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5460.12019548276317527No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5450.11997534451635627No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC5420.11931492977589928No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5420.11931492977589928No Hit
TCTGTGGAGTTGCTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5410.11909479152908027No Hit
GTCCAAGAGTCCTCTTATGTAAGACCTTGGGCAAGACCTGACGGGCGTTC5410.11909479152908027No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5390.11865451503544225No Hit
GTGGGGGAAAGCCCTACGAACCACTGAACAAATGGCACTAGTAAACTGAG5380.11843437678862324No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC5350.11777396204816624No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC5340.11755382380134725No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTGCTCTCTTTTTTGCCTTCTG5270.11601285607361422No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTCTCTAGGGGGAGCTCCCACG5240.11535244133315722No Hit
AGAAAATTGAGAGAAGTCCACCACGAGTCTAGACTCTGTGGTATTGTGAG5240.11535244133315722No Hit
ATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATATT5230.11513230308633823No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGGAGTTCCACTGCATG5220.11491216483951923No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG5210.11469202659270022No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAACAGGCTTTCACTTT5210.11469202659270022No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5200.1144718883458812No Hit
TACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACCCGC5200.1144718883458812No Hit
GTGCAGAGGTGAAGCGAAGTGCACACGGTCCGGCAGATGAGAAGGCACAG5190.1142517500990622No Hit
GTGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCT5180.1140316118522432No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC5140.11315105886496721No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTATAA5080.11183022938405318No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG5070.11161009113723418No Hit
CCATATTCTTGGGAACAAGAGCTACAGCATGGGAGGTTGGTCTTCCAAAC5070.11161009113723418No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5050.11116981464359618No Hit
CCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAAGAGTCCTCTTATGT5040.11094967639677718No Hit
TTATTATCCTGAACACATAGTTAATCATTACTTCCAAACTAGGCATTATT5040.11094967639677718No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG4990.10984898516268216No Hit
GTGCACACGGTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAA4980.10962884691586317No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCTTTTGG4980.10962884691586317No Hit
ATATGGGAGAGGCAGTAGTCGGAACAGGGTTTACTGTTCCGGAACTGGAG4910.10808787918813013No Hit
GGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGAC4870.10720732620085413No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT4860.10698718795403514No Hit
GTCCAACTGGTGACCGGGAAAGAATCCCAGAGGATTGGGAACAGAAAGAT4850.10676704970721614No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG4840.10654691146039713No Hit
GTGTACACAGAAAAGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTTA4840.10654691146039713No Hit
GTTCAGGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTA4830.10632677321357814No Hit
ACCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC4830.10632677321357814No Hit
ATCCCAGAGTGAGGGGCCTATACTTTCCTGCTGGTGGCTCCAGTTCCGGA4810.10588649671994012No Hit
GCACGATTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACA4770.10500594373266411No Hit
GTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGCAGGGTTTAAATGTATAC4750.10456566723902612No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAAT4740.10434552899220711No Hit
GGCCTATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGT4740.10434552899220711No Hit
GAATACAAGTGCAATTTCCGTCCGAAGGTTTTGTACAGCAACAAGAGGGA4720.10390525249856909No Hit
CCCCAAAAGATTGCCATGTGGTGGAGTGAACCCTGGCCCGAAGGATCCCA4720.10390525249856909No Hit
GTCATCCACAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTG4690.10324483775811209No Hit
GTACAAAACCTTCGGACGGAAATTGCACTTGTATTCCCATCCCATCATCT4680.10302469951129309No Hit
CTGTATACACAATATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGG4670.1028045612644741No Hit
CTCCTACCTGGTGTGCCGCTGGCCATTGATCCTTGTTGGGGTTGAAGTCC4660.1025844230176551No Hit
TCCTTGAAGTAGAGGACAAACGGGCAACATACCTTGGTAGTCCAGAAGAA4650.10236428477083609No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA4640.1021441465240171No Hit
GACGGGGAGACCGCGTAAAGAGAGGTGCGCCCCGTGGTCGGCCGGAACGG4630.10192400827719807No Hit
GGATAATAAGGTTTAATGCCTTTATCTAAGGGCAAATATTTGGTACGGTT4630.10192400827719807No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC4620.10170387003037908No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG4590.10104345528992208No Hit
TATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGT4580.10082331704310307No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)