FastQCFastQC Report
Sat 3 Sep 2022
EGAF00002490695

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490695
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences454260
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTAAAGGGAGCGGCAAAGCCCAAAAGCCCCACAATTCGTTGACATACT19880.43763483467617664No Hit
CCCTTTACACAATGTGGTTACCCAGCATTAATGCCTTTGTATGCATGTAT18580.4090168625897063No Hit
GTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACATTGCTGAGAGTCCAA15740.3464976004931097No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA13370.2943248359970061No Hit
GTTTAAATGTATACCCAAAGACAAAAGAAAATTGGTAACAGCGGTAAAAA13330.2934442830097301No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC13050.28728041209879807No Hit
GTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATGTGTTCAGG12770.28111654118786594No Hit
GTCTTTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA12760.280896402941047No Hit
CATTTAAACCCTGCTAAAACTAAACGTTGGGGCTACTCCCTTAACTTCAT12360.2720908730682869No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCTCCCACG11650.2564610575441377No Hit
ATTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACC10730.23620833883678952No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCCAGTACAAAGACC10630.23400695636859947No Hit
GTATACATGCATACAAAGGCATTAATGCTGGGTAACCACATTGTGTAAAG9530.2097917492185092No Hit
CCCTTAACTTCATGGGATATGTAATTGGAAGTTGGGGTACCTTACCACAG8900.19592303966891209No Hit
CTCCATATTCGGTGCAGGGTCCCCAGTCCTCGAGAAGATTGACGATATGG8840.19460221018799806No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTA8780.19328138070708406No Hit
ATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAACCTTAT8570.188658477523885No Hit
GTATAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGG8420.18535640382159996No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCT8400.18491612732796198No Hit
GGATATTATCAATAGATGTCAACAATATGTAGGCCCTCTTACAGTTAACG8250.18161405362567692No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG8160.1796328094043059No Hit
ATATGTTCCTGTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAA8000.17611059745520188No Hit
GCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT7990.17589045920838287No Hit
GTCCCGTGCTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC7550.16620437634834676No Hit
ATGTAAGACCTTGGGCAAGACCTGACGGGCGTTCACGGTGGTCTCCATGC7410.16312244089288075No Hit
CTCCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG7010.15431691102012063No Hit
GTGGTGGAGTGAACCCTGGCCCGAAGGATCCCACTCCTACCTGGTGTGCC7000.15409677277330164No Hit
TTTACATACTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACCC6830.15035442257737858No Hit
ATCTGAACCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTG6740.14837317835600758No Hit
ATGTAGGCCCTCTTACAGTTAACGAAAAAAGGAGACTAAAATTGATTATG6610.14551138114736054No Hit
AATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTACA6570.14463082816008455No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN6530.14375027517280853No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCTGATGGCC6480.14264958393871352No Hit
TCCACAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTGCTAG6450.1419891691982565No Hit
GTCTGTAAATACACAGTCTTTGAAGTATGCCTCAAGGTCGGTCGTTGACA6420.1413287544577995No Hit
GTAGTAAGCTATGTCAATGTTAATATGGGCCTAAAAATCAGACAACTATT6410.1411086162109805No Hit
ATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAATATGTT6400.14088847796416148No Hit
GTTGTCAATATGCCCTGAGCCTGAGGGCTCCACCCCAAAAGATTGCCATG6340.1395676484832475No Hit
GCACTAAGGCAAGCTATTCTGTGTTGGGGTGAGTTGATGAATCTGGCCAC6300.13868709549597147No Hit
CTGCTAGGCTGTGCTGCCAACTGGATCCTGCGCGGGACGTCCTTTGTCTA6300.13868709549597147No Hit
CTCTTACAGTTAACGAAAAAAGGAGACTAAAATTGATTATGCCTGCTAGG6270.13802668075551447No Hit
CCTTTACCCCGTTGCTCGGCAACGGTCAGGTCTTTGCCAAGTGTTTGCTG6210.13670585127460044No Hit
GTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCGGGGCCG6170.13582529828732445No Hit
ATTGACAACAGTACCAGCAGCGCCTCCTCCTGCCTCCACCAATCGGCAGT6150.13538502179368642No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCATCTGCCGGACCGTGTG6100.13428433055959144No Hit
ATGTGGTATTGGGGGCCAAGTCTGTACAACATCTTGAATCCCTTTTTACC6060.13340377757231542No Hit
CCTATAAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCT5880.1294412891295734No Hit
GCCTAATCATCTCATGTTCATGTCCCACTGTTCAAGCCTCCAAGCTGTGC5870.12922115088275435No Hit
GTGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCTTCCT5860.12900101263593536No Hit
CGGTGGTCTCCATGCGACGTGCAGAGGTGAAGCGAAGTGCACACGGTCCG5840.12856073614229735No Hit
GGTTGGGGCTTGGCCATAGGCCATCAGCGCATGCGTGGAACCTTTGTGGC5820.12812045964865937No Hit
GCACGGGACCATGCAAGACCTGCACGATTCCTGCTCAAGGAACCTCTATG5810.12790032140184035No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTC5800.12768018315502136No Hit
TCCTTTGTCTACGTCCCGTCGGCGCTGAATCCCGCGGACGACCCGTCTCG5690.12525866244001232No Hit
GGATAGAACCTAGCAGGCATAATCAATTTTAGTCTCCTTTTTTCGTTAAC5670.12481838594637432No Hit
TCCACAGAGTATGTAAATAATGCCTAGTTTGGAAGTAATGATTAACTATG5660.12459824769955531No Hit
GGTTAGGATAGAACCTAGCAGGCATAATCAATTTTAGTCTCCTTTTTTCG5650.12437810945273632No Hit
CTCCACCACATGGCAATCTTTTGGGGTGGAGCCCTCAGGCTCAGGGCATA5600.12327741821864131No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAATGTGCTGGAGTTCCACTGCATG5590.1230572799718223No Hit
CTCCTACCTGGTGTGCCGCTGGCCATTGATCCTTGTTGGGGTTGAAGTCC5510.12129617399727029No Hit
CTCCAAAAGACACCAAATACTCAAGAACAGTTTCTCTTCCAAAAGTAAGA5490.12085589750363226No Hit
TCTGTGGAGTTGCTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5450.11997534451635627No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA5320.11711354730770925No Hit
GTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACCCGCAGCGCCTCAT5290.11645313256725223No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTCTCTAGGGGGAGCTCCCACG5270.11601285607361422No Hit
CTAGTAAACTGAGCCAGGAGAAACGGACTGAGGCCCACTCCCATAGGAAT5270.11601285607361422No Hit
CAATTCTCTAGGGGGAGCTCCCACGTGTCCTGGCCAAAATTTGCAGTCCC5260.11579271782679523No Hit
CTACTGTACCTGTCTTTAATCCTGACTGGCAAACTCCCTCTTTTCCTCAC5250.11557257957997623No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTG5250.11557257957997623No Hit
GTTCAAGCCTCCAAGCTGTGCCTTGGGTGGCTTTGGGGCATGGACATTGA5240.11535244133315722No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC5230.11513230308633823No Hit
AGTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGCAGGGTTTAAATGTATA5230.11513230308633823No Hit
CTCCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCG5230.11513230308633823No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTAAGGCA5170.11381147360542421No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC5150.1133711971117862No Hit
TTCCCCCACTGTTTGGCTTTCAGTTATATGGATGATGTGGTATTGGGGGC5140.11315105886496721No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGTAGGGCTTTCCCCCACTGTT5130.1129309206181482No Hit
CTCTGTGGAAGGCTGGTATTTTATATAAGAGAGAAACTACCCGCAGCGCC5080.11183022938405318No Hit
GCTTACTACTAATTCCCTGGATGCTGGGTCTTCCAAATTACTTCCCACCC5060.11138995289041519No Hit
GGAACATATTGTACAAAAAATCAAACAATGTTTTCGGAAACTTCCTATAA5050.11116981464359618No Hit
GTGGTAAGGTACCCCAACTTCCAATTACATATCCCATGAAGTTAAGGGAG5020.11050939990313918No Hit
GTCCAACTGGTGACCGGGAAAGAATCCCAGAGGATTGGGAACAGAAAGAT5010.11028926165632016No Hit
GTCCGGCAGATGAGAAGGCACAGACGGGGAGACCGCGTAAAGAGAGGTGC5010.11028926165632016No Hit
GGACTCGTGCAGGTCTTGCATGGTCCCGTGCTGGTAGTTGATGTTCCTGG4990.10984898516268216No Hit
AAATAGACCTATTGATTGGAAAGTATGTCAACGAATTGTGGGGCTTTTGG4990.10984898516268216No Hit
CCCCAAAAGATTGCCATGTGGTGGAGTGAACCCTGGCCCGAAGGATCCCA4980.10962884691586317No Hit
GCATTAATGCCTTTGTATGCATGTATACAAGCTAAACAGGCTTTCACTTT4980.10962884691586317No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTACGAACCACTGAACA4970.10940870866904416No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4910.10808787918813013No Hit
GTTCTATCCTAACCGTACCAAATATTTGCCCTTAGATAAAGGCATTAAAC4880.10742746444767313No Hit
CCCATAGGAATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG4880.10742746444767313No Hit
GTGTACACAGAAAAGCCTTGTAAGTTGGCGAGAAAGTGAAAGCCTGTTTA4860.10698718795403514No Hit
ATATAAGAGAGAAACTACCCGCAGCGCCTCATTTTGTGGGTCACCATATT4860.10698718795403514No Hit
TACCTTGGTAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4860.10698718795403514No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4860.10698718795403514No Hit
GAATATGGAGAGCACCACATCAGGATTCCTAGGACCCCTGCTCGTGTTAC4790.10544622022630211No Hit
GGCCTACATATTGTTGACATCTATTGATAATATCCTCTTTCAAATGAATG4740.10434552899220711No Hit
GTTAAGGGAGTAGCCCCAACGTTTAGTTTTAGCAGGGTTTAAATGTATAC4720.10390525249856909No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTGCTCTCTTTTTTGCCTTCTG4710.10368511425175009No Hit
GGCCTATACTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGT4700.1034649760049311No Hit
ATCCCAGAGTGAGGGGCCTATACTTTCCTGCTGGTGGCTCCAGTTCCGGA4660.1025844230176551No Hit
ATATGGGAGAGGCAGTAGTCGGAACAGGGTTTACTGTTCCGGAACTGGAG4650.10236428477083609No Hit
GTCATCCACAGGCCATGCAGTGGAACTCCAGCACATTCCACCAAGCTCTG4650.10236428477083609No Hit
GGTCTATTTATAGGAAGTTTCCGAAAACATTGTTTGATTTTTTGTACAAT4610.10148373178356007No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT4560.10038304054946506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)