FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490696

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490696
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386062
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT16650.43127787764659564No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13900.36004579575301376No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG11850.30694551652325275No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11140.2885546881070916No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAC11120.288036636602411No Hit
GTACGGGACCATGCAAGACATGCACGTCTCCTGCTCAAGGAACCTCTATG10600.2745672974807155No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC10440.27042288544327076No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC10340.2678326279198678No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC10010.25928477809263795No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTTTGGGTCACCATATT9930.2572125720739156No Hit
CTTATATAGAATACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGTTT9920.2569535463215753No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT9670.25047790251306784No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA9490.2458154389709425No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTTTGGGTCACCATA9030.2339002543632888No Hit
ATCTTAACCTCTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG8980.2326051256015873No Hit
CCCATAGGTATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG8970.232346099849247No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA8360.21654552895648888No Hit
GGTTAAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7780.2015220353207516No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATGTCCTCCTGC7740.2004859323113904No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7740.2004859323113904No Hit
CCCTTAACTTTATGGGATATGTGATTGGGAGTTGGGGTACTTTACCACAG7600.19685957177862623No Hit
GTTTTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA7490.19401028850288296No Hit
ATTTACATACTTTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACA7380.1911610052271397No Hit
CTACTATACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC7250.18779367044671583No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCAGG7220.1870165931896949No Hit
CTCTTATATAGAATACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGT7190.18623951593267404No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA6810.17639653734374272No Hit
GTTGTCAGTATGCCCTGAGTCTGAGGGCTCCACCCCAAAAGACCGCCGTG6710.17380627982033972No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6670.17277017681097856No Hit
ACCTATGGGAGTGGGCCTCAGCCCGTTTCTCCTGGCTCAGTTTACTAGTG6570.17017991928757556No Hit
GTGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCGCCATGC6550.16966186778289497No Hit
GGGTAGAGGTTAAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA6450.167071610259492No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6370.16499940424076961No Hit
ACTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCACCACCAATCGGCAGT6260.16215012096502635No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACGTTCCACCAAGCTCTGCTAG6200.16059596645098456No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC6120.15852376043226216No Hit
TCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGG6080.157487657422901No Hit
GGATTAAAGACAGGTATAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC5860.15178909087141443No Hit
CTCCCATAGGTATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5850.15153006511907413No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5560.1440183183012055No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG5420.14039195776844132No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5340.13831975174971894No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATA5270.13650657148333686No Hit
TTTACATACTTTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACAC5260.1362475457309966No Hit
CCCACGTGTCCTGGCCAAAATTCGCAGTCCCCAACCTCCAATCACTCACC5160.1336572882075936No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA5100.1321031336935518No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5090.1318441079412115No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC5080.13158508218887122No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG5040.13054897917951003No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC5030.13028995342716973No Hit
CTCCAAAAGACACCAAATACTCAAGTACAGTTTCTCTTCCAAAAGTAAGA5030.13028995342716973No Hit
GATCATTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC4990.12925385041780854No Hit
TCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT4950.12821774740844735No Hit
CCTCTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG4910.12718164439908614No Hit
GACCTTTAACCTAATCTCCTCCCCCAACTCCTCCCAGTCTTTAAACAAAC4760.12329625811398168No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT4730.1225191808569608No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4720.1222601551046205No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4660.12070600059057872No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT4650.12044697483823842No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT4640.12018794908589811No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC4630.11992892333355781No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4620.11966989758121752No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4600.11915184607653694No Hit
GTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGGATGAAGAGGA4570.11837476881951603No Hit
GTACTAAAACTCAAACAATGTTTTCGAAAACTTCCTGTAAATAGACCTAT4540.11759769156249515No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4530.11733866581015485No Hit
CTTTTTACCTCTATTACCAATTTTCTTGTGTCTTTGGGTATACATTTGAA4510.11682061430547425No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4430.11474840828675187No Hit
CCTATGGGAGTGGGCCTCAGCCCGTTTCTCCTGGCTCAGTTTACTAGTGC4430.11474840828675187No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4320.1118991250110086No Hit
GTATAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA4310.1116400992586683No Hit
GGCCTACATATTGTTGACATCTATTGATAATGTCCTCCTGCAAATGAATG4300.111381073506328No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAACGTTGTGGAGTTCCACTGCATG4290.11112204775398771No Hit
ACTATACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACAT4260.1103449704969668No Hit
GTGTAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC4250.11008594474462652No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4230.10956789323994592No Hit
TATTAACATTGACATAGCTGACTACTAATTCTCTGGATGCTGGGTCTTCC4200.10879081598292502No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4190.10853179023058472No Hit
CCCCTATCTTATCAACACTTCCGGAAACTACTGTTGTTAGACGACGAGGC4170.10801373872590414No Hit
GTCTTTAAACAAACAGTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACA4140.10723666146888323No Hit
CTTCTGGACTATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAAC4140.10723666146888323No Hit
CTAGTAAACTGAGCCAGGAGAAACGGGCTGAGGCCCACTCCCATAGGTAT4140.10723666146888323No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTA4120.10671860996420263No Hit
ACATAGAGGTTCCTTGAGCAGGAGACGTGCATGTCTTGCATGGTCCCGTA4110.10645958421186236No Hit
CCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAA4100.10620055845952205No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4100.10620055845952205No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT4080.10568250695484145No Hit
GTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCCGCCTGTAACACG4070.10542348120250115No Hit
GGAAAATACAGGCCCCTCACTCTGGGATCTAGCAGAGCTTGGTGGAACGT4050.10490542969782055No Hit
GTGTAAACAATATCTTAACCTCTACCCCGTTGCCCGGCAACGGTCAGGTC4010.10386932668845937No Hit
CACGTCTCCTGCTCAAGGAACCTCTATGTTTCCCTCTTGTTGCTGTACAA3980.10309224943143847No Hit
GTGGTAAAGTACCCCAACTCCCAATCACATATCCCATAAAGTTAAGGGAG3950.10231517217441759No Hit
CTTGTATTCCCATCCCATCATCTTGGGCTTTCGCAAGATACCTATGGGAG3900.1010200434127161No Hit
GGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGACGCGGCGA3890.1007610176603758No Hit
GTGTTGGGGTGAGTTGATGAACCTGGCCACCTGGGTGGGAAGTAATTTGG3880.1005019919080355No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)