FastQCFastQC Report
Fri 2 Sep 2022
EGAF00002490697

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002490697
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences386062
Sequences flagged as poor quality0
Sequence length35-151
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTGTAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT14870.3851712937300226No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13800.3574555382296108No Hit
GTATGTAAATAATGCCTAGTTTTGAAGTAATGATTAACTGCATGTTCCGG11800.3056503877615513No Hit
GTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGGGGAGCACCCACG11630.3012469499717662No Hit
ATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAACCTTAC10590.27430827172837524No Hit
GTACGGGACCATGCAAGACATGCACGTCTCCTGCTCAAGGAACCTCTATG10240.2652423703964648No Hit
GTGTAAAAGGGGCAGCAAAGCCCAAAAGACCCACAATTCTCTGACATACT9740.25229108277944995No Hit
AGTCTAGACTCTGTGGTATTGTGAGGATTCTTGTCAACAAGAAAAACCCC9730.2520320570271096No Hit
CCTCCCGCTTACAGACCACCAAATGCCCCTATCTTATCAACACTTCCGGA9610.24892374799902606No Hit
ATATAAGAGAGAAACTACACGCAGCGCCTCATTTTTTGGGTCACCATATT9590.24840569649434546No Hit
ATCTTAACCTCTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTG9390.2432251814475395No Hit
CTTATATAGAATACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGTTT9330.24167102693349773No Hit
GTCCCGTACTGGTAGTTGATGTTCCTGGAAGTAGAGGACAAACGGGCAAC9270.24011687241945592No Hit
GTGCTGGTGAACAGACCAATTTATGCCTACAGCCTCCTAGTACAAAGATC8810.22820168781180222No Hit
GTATACATGCATACAAAGGCATTAAGGCAGGATAGCCACATTGTGTAAAA8530.2209489667462739No Hit
GTTTTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA8500.22017188948925304No Hit
CTATATAAGAGAGAAACTACACGCAGCGCCTCATTTTTTGGGTCACCATA8360.21654552895648888No Hit
CTCTTATATAGAATACCAGCCTTCCACAAAGTATGTAAATAATGCCTAGT7970.20644352461521723No Hit
CCCATAGGTATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAGTG7590.19660054602628593No Hit
GGTTAAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGAGAAAGTG7520.19478736575990385No Hit
ATTTACATACTTTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACA7470.19349223699820237No Hit
CCCTTAACTTTATGGGATATGTGATTGGGAGTTGGGGTACTTTACCACAG7360.1906429537224591No Hit
GGTTAGGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCA7340.19012490221777847No Hit
CTGTAAGAGGGCCTACATATTGTTGACATCTATTGATAATGTCCTCCTGC7250.18779367044671583No Hit
CTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCACATTCATTTGCAGG6960.18028192362884718No Hit
CTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATGCCTGCTAGG6520.16888479052587407No Hit
GTTGTCAGTATGCCCTGAGTCTGAGGGCTCCACCCCAAAAGACCGCCGTG6390.1655174557454502No Hit
GTATCGGGAGGCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAG6270.16240914671736664No Hit
CTACTATACCTGTCTTTAATCCTGAGTGGCAAACTCCCTCCTTTCCTCAC6190.16033694069864426No Hit
ACTGACAACAGTGCCAGCAGCGCCTCCTCCTGCCACCACCAATCGGCAGT6100.1580057089275816No Hit
ACCTATGGGAGTGGGCCTCAGCCCGTTTCTCCTGGCTCAGTTTACTAGTG6010.1556744771565189No Hit
CCTCTACCCCGTTGCCCGGCAACGGTCAGGTCTCTGCCAAGTGTTTGCTG5950.15412032264247713No Hit
GGGTAGAGGTTAAGATATTGTTTACACAGAAAGGCCTTGTAAGTTGGCGA5930.1536022711377965No Hit
TCCTCAGGCCATGCAGTGGAACTCCACAACGTTCCACCAAGCTCTGCTAG5790.14997591060503235No Hit
GTCTTTGAAGTACGCCTCAAGGTCGGTCGTTGACATTGCTGGGAGTCCAA5750.14893980759567116No Hit
CATGTATACAAGCTAAACAGGCTTTCACTTTCTCGCCAACTTACAAGGCC5680.14712662732928908No Hit
ATACCACATCATCCATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTG5600.1450544213105667No Hit
CTCCCATAGGTATCTTGCGAAAGCCCAAGATGATGGGATGGGAATACAAG5380.13935585475908016No Hit
TCTTTTGTCTTTGGGTATACATTTGAACCCTAATAAAACCAAACGTTGGG5350.13857877750205924No Hit
AGTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATA5330.13806072599737867No Hit
TCTGTGGAGTTACTCTCTTTTTTGCCTTCTGACTTCTTTCCTTCTATTCG5120.1326211851982324No Hit
GTGTAAGACCTTGGGCAAGACCTGGTGGGCGTTCACGGTGGTCGCCATGC5070.13132605643653092No Hit
TCCTAGCAGCCATGGAAAGGAGGTGTATTTCCGAGAGAGGACAACAGAGT5060.13106703068419062No Hit
GTACTAAAACTCAAACAATGTTTTCGAAAACTTCCTGTAAATAGACCTAT5000.12951287617014884No Hit
TTTACATACTTTGTGGAAGGCTGGTATTCTATATAAGAGAGAAACTACAC4860.12588651563738468No Hit
GGATTAAAGACAGGTATAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCAC4850.12562748988504435No Hit
CTTTTGGAGTGTGGATTCGCACTCCTCCCGCTTACAGACCACCAAATGCC4810.12459138687568319No Hit
GTATAAAGAATTTGGAGCTTCTGTGGAGTTACTCTCTTTTTTGCCTTCTG4800.12433236112334288No Hit
GTTCTATCCTAACCTTACCAAATATTTGCCCTTAGACAAAGGCATTAAAC4780.12381430961866229No Hit
CCTATGGGAGTGGGCCTCAGCCCGTTTCTCCTGGCTCAGTTTACTAGTGC4780.12381430961866229No Hit
CTCTTTACGCGGTCTCCCCGTCTGTGCCTTCTCGTCTGCCGGACCGTGTG4770.12355528386632199No Hit
ATGTAGGCCCTCTTACAGTTAATGAAAAAAGGAGATTAAAATTAATTATG4730.1225191808569608No Hit
ATGTTAATATGGGCCTAAAAATCAGACAACTATTGTGGTTTCACATTTCC4720.1222601551046205No Hit
CATTTGAACCCTAATAAAACCAAACGTTGGGGCTACTCCCTTAACTTTAT4720.1222601551046205No Hit
CTTTTACACAATGTGGCTATCCTGCCTTAATGCCTTTGTATGCATGTATA4640.12018794908589811No Hit
CTCCAAAAGACACCAAATACTCAAGTACAGTTTCTCTTCCAAAAGTAAGA4560.11811574306717573No Hit
ATATAACTGAAAGCCAAACAGTGGGGGAAAGCCCTGCGAACCACTGAACA4550.11785671731483544No Hit
GTTTACTAGTGCCATTTGTTCAGTGGTTCGCAGGGCTTTCCCCCACTGTT4540.11759769156249515No Hit
TACCTTGATAGTCCAGAAGAACCAACAAGAAGATGAGGCATAGCAGCAGG4540.11759769156249515No Hit
ATCAAGGTATGTTGCCCGTTTGTCCTCTACTTCCAGGAACATCAACTACC4490.11630256280079365No Hit
CTTTTTACCTCTATTACCAATTTTCTTGTGTCTTTGGGTATACATTTGAA4450.11526645979143246No Hit
GCCTTAGAGTCTCCGGAACATTGTTCACCTCACCATACAGCACTCAGGCA4400.11397133102973099No Hit
GTCTAAGGGCAAATATTTGGTAAGGTTAGGATAGAACCTAGCAGGCATAA4390.11371230527739068No Hit
GTGTAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACATC4340.11241717651568918No Hit
CCTCTATGTTTCCCTCTTGTTGCTGTACAAAACCTTCGGACGGAAATTGC4320.1118991250110086No Hit
CCCTTGGACTCATAAGGTGGGAAACTTTACTGGGCTTTATTCTTCTACTA4300.111381073506328No Hit
GTGTAAACAATATCTTAACCTCTACCCCGTTGCCCGGCAACGGTCAGGTC4270.1106039962493071No Hit
GGTAAAAAGGGACTCAAGATGTTGTACAGACTTGGCCCCCAATACCACAT4270.1106039962493071No Hit
GTACAGACTTGGCCCCCAATACCACATCATCCATATAACTGAAAGCCAAA4260.1103449704969668No Hit
GTATGGATCGGCAGAGGAGCCACAAAGGTTCCACGCATGCGCCGATGGCC4260.1103449704969668No Hit
GTATTTTCCTGCTGGTGGCTCCAGTTCCGGAACAGTAAACCCTGTTCCGA4240.10982691899228622No Hit
GTATAGTAGAAGAATAAAGCCCAGTAAAGTTTCCCACCTTATGAGTCCAA4200.10879081598292502No Hit
CTTTTTACCTCTATTACCAATTTTCTTTTGTCTTTGGGTATACATTTGAA4200.10879081598292502No Hit
CTCTGGGATCTAGCAGAGCTTGGTGGAACGTTGTGGAGTTCCACTGCATG4180.10827276447824444No Hit
CCTTATGAGTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCT4120.10671860996420263No Hit
GGCCTACATATTGTTGACATCTATTGATAATGTCCTCCTGCAAATGAATG4070.10542348120250115No Hit
TATTAACATTGACATAGCTGACTACTAATTCTCTGGATGCTGGGTCTTCC4050.10490542969782055No Hit
GGATAGAACCTAGCAGGCATAATTAATTTTAATCTCCTTTTTTCATTAAC4040.10464640394548028No Hit
GTTAAGGGAGTAGCCCCAACGTTTGGTTTTATTAGGGTTCAAATGTATAC4030.10438737819313997No Hit
GTCCAAGGGATACTAACATTGAGATTCCCGAGATTGAGATCTTCTGCGAC4010.10386932668845937No Hit
CAATACCACAGAGTCTAGACTCGTGGTGGACTTCTCTCAATTTTCTAGGG3980.10309224943143847No Hit
GCCTGAGGATGACTGTCTCTTAGAGGTGGAGAGATGGGAGTAGGCTGTCT3970.10283322367909818No Hit
CTATTGTGGTTTCACATTTCCTGTCTTACTTTTGGAAGAGAAACTGTACT3880.1005019919080355No Hit
ATCATATTCCTCTTCATCCTGCTGCTATGCCTCATCTTCTTGTTGGTTCT3870.1002429661556952No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)