FastQCFastQC Report
Sat 1 Apr 2023
EGAF00002493211

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002493211
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37397151
Sequences flagged as poor quality0
Sequence length75
%GC37

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCAAAAGTCTGAAATCCAGTCAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG22654216.0577368580831195RNA PCR Primer, Index 9 (97% over 39bp)
GATCGGAAGAGCAAAAGTCTGAAATAAAGTCAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG17282654.621381452292984RNA PCR Primer, Index 9 (97% over 36bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG7077481.8925184969304212No Hit
GATCGGAAGAGCAAAAGTATGAAATAAAGTCAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG6868001.836503534721134TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCAAAAGTCTGAACTCCAGTCAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG5738631.534509941679782TruSeq Adapter, Index 9 (97% over 47bp)
GATCGGAAGAGCAAAAGTATGAAATCCAGTCAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG5555311.4854901647454375TruSeq Adapter, Index 9 (97% over 39bp)
GATCGGAAGAGCAAAAGTCTGAAATCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG4214671.1270029634075602TruSeq Adapter, Index 9 (97% over 47bp)
GATCGGAAGAGCAAAAGTCTGAAATAAAGTAAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG4180881.1179675157607594Illumina PCR Primer Index 9 (96% over 32bp)
GATCGGAAGAGCAAAAGTATGAACTCCAGTCAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG2977450.7961702751099944Illumina PCR Primer Index 9 (97% over 43bp)
GATCGGAAGAGCAAAAGTCTGAAATAAAGTCAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACCG2688700.7189585110373782RNA PCR Primer, Index 9 (97% over 36bp)
GATCGGAAGAGCAAAAGTCTGAAATCCAGTCAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG2603340.6961332428772449RNA PCR Primer, Index 9 (97% over 39bp)
GATCGGAAGAGCAAAAGTCTGAAATAAAGTCAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG1881030.5029875136745042RNA PCR Primer, Index 9 (97% over 36bp)
GATCGGAAGAGCAAAAGTATGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1855280.49610196241954363TruSeq Adapter, Index 9 (97% over 47bp)
GATCGGAAGAGCAAAAGTATGAAATCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1821700.4871226687829776Illumina PCR Primer Index 9 (97% over 43bp)
GATCGGAAGAGCAAAAGTCTGAACTCCAGTCAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACG1758390.47019357169748044TruSeq Adapter, Index 9 (97% over 47bp)
GATCGGAAGAGCAAAAGTCTGAACTCCAGTCAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACCG1621250.4335223290137797TruSeq Adapter, Index 9 (97% over 47bp)
GATCGGAAGAGCAAAAGTATGAAATAAAGTAAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1608540.43012367439434085Illumina PCR Primer Index 9 (96% over 32bp)
GATCGGAAGAGCAAAAGTATGAAATAAAGTCAAGATAAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1323170.3538157224864536Illumina PCR Primer Index 9 (96% over 30bp)
GATCGGAAGAGCAAAAGTATGAAATAAAGTCAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACG1090270.29153825113576165TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCAAAAGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG1039990.2780933766852988TruSeq Adapter, Index 9 (100% over 47bp)
GATCGGAAGAGCAAAAGTATGAAATAAAGTCAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG986960.2639131521008111TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCAAAAGTCTGAACTCCAGTAAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG858020.22943458981674833RNA PCR Primer, Index 9 (96% over 32bp)
GATCGGAAGAGCAAAAGTATGAAATAAAGTCAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACCG826160.22091522426400875TruSeq Adapter, Index 9 (97% over 36bp)
GATCGGAAGAGCAAAAGTCTGAACTCCAGTCAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG769710.2058204915128428TruSeq Adapter, Index 9 (97% over 47bp)
GATCGGAAGAGAAAAAGTATGAAATAAAGTCAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG566480.15147677960815784Illumina PCR Primer Index 9 (100% over 30bp)
GATCGGAAGAGCACAAGTCTGAACTCCAGTCAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG514310.13752651906558336Illumina PCR Primer Index 9 (97% over 43bp)
GATCGGAAGAGCAAAAGTATGAAATAAAGTAAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG509120.1361387128126418Illumina PCR Primer Index 9 (96% over 32bp)
GATCGGAAGAGCAAAAGTATGAAATAAAGTAAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACCG492980.13182287602603737Illumina PCR Primer Index 9 (96% over 32bp)
GATCGGAAGAGCAAAAGTATGAAATAAAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACCG441450.11804375151465414Illumina PCR Primer Index 9 (97% over 37bp)
GATCGGAAGAGCAAAAGTCTGAATTCCAGTCAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG427200.11423330082016141RNA PCR Primer, Index 9 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)