FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002493329

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002493329
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8804913
Sequences flagged as poor quality0
Sequence length75
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGCTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG100702911.437126068139458TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG6782107.702631474041823TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGCTCTCGTATGCCGTCTTCTGCTTGAAAAAAAACCGG6531637.418165290219222TruSeq Adapter, Index 9 (98% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG4119844.679024085757576No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAACCGG2621992.977871558753619TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGCTCTCGTATGCCGTCTTCTGCTTGAAAAAAACCCGG770320.8748751975175677TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGCTCTCGTATGCCGTCTTCTGCTTGAAAAAAACACGG471360.5353374871506397TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG471070.5350081255771636TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGCTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGG394970.4485791057787851TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAACCCGG228690.2597299939249825TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGCTCTCGTATGCCGTCTTCTGCTTGAAAAAACACCGG216780.24620345482118905TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAACACGG195800.22237584857454015TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTAGCTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG162090.184090404981855TruSeq Adapter, Index 9 (97% over 39bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG156570.17782117779017237TruSeq Adapter, Index 9 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGCTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG150970.17146109223339287Illumina PCR Primer Index 9 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGATCAGCTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG147410.16741789498658308TruSeq Adapter, Index 9 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG142110.16139852829891677TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGCTCTCGTATGCCGTCTTCTGCTTGAAAAAAAACAGG138850.1576960499212201TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGATCAGCTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG136280.15477722494248383TruSeq Adapter, Index 9 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGCTCTCGTATGCCGTCTTCTGCTTGAAAAAACAACGG123750.1405465335091897TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG120980.13740056261771127TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACGATCAGCTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG117830.1338230144920228Illumina PCR Primer Index 9 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGATCAGCTCTCGTATGCCGTCTTCTGCTTGAAAAAAAACCGG106170.12058040777915693TruSeq Adapter, Index 9 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAACAGG104120.11825216217355015TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG103640.11770701198296905TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGATCAGCTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG102940.1169120012883716TruSeq Adapter, Index 9 (97% over 39bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTAGCTCTCGTATGCCGTCTTCTGCTTGAAAAAAAACCGG102780.11673028455817791TruSeq Adapter, Index 9 (97% over 39bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG99310.11278930297210206TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG98010.11131285453927825TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGATCAGCTCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG93630.1063383590502257Illumina PCR Primer Index 9 (97% over 43bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG90150.10238602016851273TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGATCAGCTCTCGTATGCCGTCTTCTGCTTGAAAAAAAACCGG88620.10064835393603548TruSeq Adapter, Index 9 (97% over 39bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)