FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002493357

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002493357
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17964655
Sequences flagged as poor quality0
Sequence length75
%GC40

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAACCCCCG13722157.638415544300739TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAACCCCG10895866.0651651812962735TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAACCCGG5336692.9706610007261482TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAACCCG4473192.489994937281011TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAACCCCGG4267762.375642616014613TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAACCGG3235051.8007860434837184TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAACACCCG1198370.6670709790975669TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGCAAAAACCCCCG861650.47963626354082506TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAACCCCCC672560.3743795803481893TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACAAGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAACCCCG566550.3153692625881209TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAACACCGG522180.2906707643425382TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAACACCG516800.2876759948910792TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACCG480510.26747521730865415TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAACCCCC417840.23259004973933536TruSeq Adapter, Index 9 (100% over 63bp)
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG372030.20708997751417996No Hit
GATCGGAAGAGCACAAGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAACCCCCG356930.19868458370060543TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAACGG332180.18490753092670023TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAACCCCCG302950.16863669243856896TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAACACGG295530.16450635984938203TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAACCCCCG278860.15522702773863456TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAACCCCCG257410.14328691533458338TruSeq Adapter, Index 9 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAACCCCCG251110.13978002917395296TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCTAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAACCCCCG238390.1326994590210611TruSeq Adapter, Index 9 (98% over 63bp)
GATTGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAACCCCG228020.12692701307094403TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAATTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAACCCCCG215710.12007466884279157TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCAAGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAACCCCCG215330.11986314237595992TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATTAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAACCCCG215090.11972954671269778TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAACCACCG213840.11903373596654097TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGCAAAAACCCCGG205060.1141463612855354TruSeq Adapter, Index 9 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTTCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAACCCCG196610.10944268064151524TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGTACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAACCCCG194190.10809559103695562TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCAAACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAACCCCG192680.10725505165559818TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACAAGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAAAACCCG188750.1050674226696811TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACATGTCTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAACCCCCG188090.1047000345957103TruSeq Adapter, Index 9 (98% over 63bp)
GATCGGAAGAGCACACGTTTGAACTCCAGTCACGATCAGATCTCGTATGCCGTCTTCTGCTTGAAAAAACCCCCG182170.10140467490191156TruSeq Adapter, Index 9 (98% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)