FastQCFastQC Report
Sat 13 May 2023
EGAF00002493666

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002493666
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39121576
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7356891.8805198440880806TruSeq Adapter, Index 10 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC1441180.3683849546347519TruSeq Adapter, Index 10 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1069240.2733120976516897TruSeq Adapter, Index 10 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT1045940.26735630486869955TruSeq Adapter, Index 10 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA713060.18226770823343108TruSeq Adapter, Index 10 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAGA419410.10720682622806402TruSeq Adapter, Index 10 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAATA416520.10646810343223391TruSeq Adapter, Index 10 (100% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)