FastQCFastQC Report
Tue 19 Sep 2023
EGAF00002493674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002493674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences56969330
Sequences flagged as poor quality0
Sequence length75
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAA7820351.372729853063043TruSeq Adapter, Index 10 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAC1633010.2866472187754358TruSeq Adapter, Index 10 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAT1191960.20922836901890893TruSeq Adapter, Index 10 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAAAG1155000.20274066765398152TruSeq Adapter, Index 10 (100% over 63bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCCGTCTTCTGCTTGAAAAAAAAAACA853520.14982096507015266TruSeq Adapter, Index 10 (100% over 63bp)

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)