FastQCFastQC Report
Sat 13 May 2023
EGAF00002495186

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002495186
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34692433
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA2654180.7650602077980521No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGC1547100.4459473914671825TruSeq Adapter, Index 2 (100% over 50bp)
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG1406560.40543711650318676No Hit
GGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTC1284700.370311301026365No Hit
CTCAGGAGTTTGATAGTTCTTGGGCAGTGAGAGTGAGTAGTAGAATGTTT858740.24752948287022708No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT720330.20763317464647119No Hit
GGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGT670560.19328710673016217No Hit
GCCTGGTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTC663830.1913472024288409No Hit
GGCAAATTTAAAGTTGAACTAAGATTCTATCTTGGACAACCAGCTATCAC603380.17392265339245594No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT568960.1640011814680164No Hit
GGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCAG562710.162199635868721No Hit
CCTGGTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTCC540370.15576019127859958No Hit
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT524670.15123470873316958No Hit
GGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTCG502940.14497109499353936No Hit
GGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGG485380.13990947247775906No Hit
GGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAA473330.13643609256231756No Hit
GGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTA467210.13467201911148752No Hit
GGGACGATTAGTTTTAGCATTGGAGTAGGTTTAGGTTATGTACGTAGTCT464180.1337986298049491No Hit
GTCGCCTGGTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTA458660.13220750473165144No Hit
GTCCAATTGGGTGTGAGGAGTTCAGTTATATGTTTGGGATTTTTTAGGTA432810.12475631213296572No Hit
GTGGTGATTAGTCGGTTGTTGATGAGATATTTGGAGGTGGGGATCAATAG426050.12280776041276782No Hit
GTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTA417770.12042107280282129No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC386420.11138452007675564TruSeq Adapter, Index 2 (100% over 50bp)
GGGCAGGATAGTTCAGACGGTTTCTATTTCCTGAGCGTCTGAGATGTTAG386170.11131245825278382No Hit
GGCGCTTCCAATTAGGTGCATGAGTAGGTGGCCTGCAGTAATGTTAGCGG384740.11090026461966504No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)