FastQCFastQC Report
Tue 27 Sep 2022
EGAF00002495187

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002495187
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34692433
Sequences flagged as poor quality0
Sequence length101
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG1381420.398190579484581Illumina Single End PCR Primer 1 (100% over 50bp)
CGCAAAACTAACCCCCTAATAAAATTAATTAACCACTCATTCATCGACCT675630.19474852052031058No Hit
AGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCT657460.18951106715403904No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN602560.17368629060982838No Hit
CTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCATGA586890.169169455483275No Hit
GTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCACCTTTCAT517380.14913338594615141No Hit
CTAAATACTACCGTATGGCCCACCATAATTACCCCCATACTCCTTACACT503310.14507774649301766No Hit
CTTTTCCTAACACTCACAACAAAACTAACTAATACTAACATCTCAGACGC364980.10520449805293275No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)