FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002495373

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002495373
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48453567
Sequences flagged as poor quality0
Sequence length35-151
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCTTATCTCGTAT2936140.6059698349968745TruSeq Adapter, Index 20 (97% over 44bp)
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN895740.18486564673350056No Hit
GGCCTGGATGGTTCAGGATAATCACCTGAGCAGTGAAGCCAGCTGCTTCC511230.10550926003032965No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)