FastQCFastQC Report
Wed 28 Dec 2022
EGAF00002528710

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002528710
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences478276348
Sequences flagged as poor quality0
Sequence length151
%GC25

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCCCGTATGCC65673581.3731304145527181TruSeq Adapter, Index 7 (98% over 50bp)
TTGTAAATTATGGTGTTTATTTTGAAGGAGTTGTTAGTGATAATTGGAGT36931880.7721870453021022No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACCAGATCATCTCGTATGCC24675200.5159193027876846TruSeq Adapter, Index 7 (100% over 50bp)
TTGTAAATTATTGAATTGTTTGTGTTTATTTTGTGTGTATGTGTAGGAAA20730640.43344480835585875No Hit
TTGTAAATTATGTATGTTGGATTTTGTAGGATATTTTTGTTTTTTTGTTT15393900.3218620378024631No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN13140560.27474827168329885No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)