FastQCFastQC Report
Fri 6 Jan 2023
EGAF00002528724

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002528724
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences487861104
Sequences flagged as poor quality0
Sequence length151
%GC23

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTAGTTGATATGGTGTTTATTTTGAAGGAGTTGTTAGTGATAATTGGAGT64108311.3140688912145781No Hit
GTAGTTGATATTGAATTGTTTGTGTTTATTTTGTGTGTATGTGTAGGAAA35949980.736889653740463No Hit
GTAGTTGATATGTATGTTGGATTTTGTAGGATATTTTTGTTTTTTTGTTT28630120.5868498178120796No Hit
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCTCGTATGCC24486790.5019213419399797TruSeq Adapter, Index 6 (100% over 50bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGCCAATATCCCGTATGCC8685940.17804124839597787TruSeq Adapter, Index 6 (98% over 50bp)
GTAGTTGATATGTATGTTGGATTTTGTGGGATATTTTTGTTTTTTTGTTT5232240.10724855818798787No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)