FastQCFastQC Report
Fri 9 Dec 2022
EGAF00002528829

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002528829
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences503871008
Sequences flagged as poor quality0
Sequence length151
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCGT35774840.7099999688809244Illumina Single End PCR Primer 1 (100% over 50bp)
TGCATCAGCGCTGAATTGTTCGCGTTTACCTTGCGTGTACGCGCAGGAAA16845290.334317508500112No Hit
TGCATCAGCGCGGCGTCCATCTCGAAGGAGTCGCCAGCGATAACCGGAGT13442130.26677720659808235No Hit
TGCATCAGCGCGTACGCTGGACTTTGTGGGATACCCTCGCTTTCCTGCTC5168940.10258458847467564No Hit
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC5040450.10003453105998111No Hit

[OK]Adapter Content

Adapter graph