FastQCFastQC Report
Sun 5 Mar 2023
EGAF00002529134

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529134
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89856233
Sequences flagged as poor quality0
Sequence length1-144
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATATCATTCTTGCCTGTGTACATACATCTCTTCTCCACTGCTCAGAAC17942161.9967629847113668No Hit
AGACATTTCAAAATGACCCTGTAGGCAAGGCCCAGACATAAAAGTTACAT15527541.72804261669861No Hit
CTCTGGCTTGATCCTTGTATATGAGCCCTAGCATCCAGTCCAAGTATTTC15489351.7237924941723297No Hit
GATTATGGTCGCTGAAAAATCTTTGTCAGAGGCAGTTGGGATAACCAGCC14844601.6520389854313169No Hit
CTGTGCAGGAGTACATCATTACAGCAATCAAAGAAGTACTGAGGATTCAC14412731.6039766545744245No Hit
CTCTGAGAAGCATTCCATTCTGTAAAATTTCCCGGCATGTGCATCTTATG14339231.5957969215112766No Hit
CTATAGTTGAAGATGAGATTTAGGTGGGGACACAGCCAAACAATGTAAGA14147281.574435019994662No Hit
ATTGGTACTTGCAACACTTTCTCTGGAATTTGGCCATTTCTCTGCTCTTT13902861.5472337906709266No Hit
CCGTCTCTTGTATCAAATCAATTTTGGAATGAAGTGAGGATATAAACAAG13877131.5443703276543987No Hit
GTGATGTTTGGCACAATCGGATTGGTTCCCATGAGGTCCAGGGCTGCGGC13520341.5046635662992907No Hit
AGTGTGACATGCTCATAGAGACTTCCTCTTGAAGCATACAGAATGGAGAG13162751.464867773836012No Hit
GTACGTTTGCTTTTGTGGTAATTTCTCTGGCCATGCTCTCCCTTCCCTCC13091061.4568894736550997No Hit
ACTGCTTCTCAACTAGGAGGATTTTTGTCCCCTCTGGGTACGGGCAATGT12451901.3857580697824268No Hit
TAGAAGGGTCTATGCTGTGAAGATTGTTGTTTACCAGTTTTATGCACTCT12174551.3548920974686307No Hit
GGTCTAGAGTGATGGTGTCCTTCTCAAGCGTCTAAGTCCTGTCCAGGCTA11789071.3119924579967646No Hit
ATTGTGTGTCATCCAAAAGACAAACAGACTGGCCGAATAGAAAGCAGATC11390381.2676226923512361No Hit
AACTTCGGTTGACAATGATCCTGCTCAGTGGTCTCCATCGTTTTTGCCAT10837041.206042100607534No Hit
GCTGAAGCACAATCACTTAGTATATTTCCTTACAGTCTTCTTTCAAATGC10750871.1964523373687388No Hit
TATGGAAAATGTCAGACCCTTCCACAAAGTTAGAGTAGTGTAATGAACCA10632471.1832757333595323No Hit
TGCATTGGCATGTTACATGATACTGTGTATAGGAGAAAGTGATGAAGAGA10602611.1799526472470754No Hit
AATGCTTGAAACTCCTGAACAGCCCTGGCTCCTCACACTTCCTGCCATCC10493111.1677665143162634No Hit
ATGATGCATGGAGGAACAGGTGGGAAGGTGGCCCAGTCACAGTCCCCACC10155921.1302410151113278No Hit
GCTGTTTAGCAGTCTTCACTGTTGATGCCTTCAGGTACTGACAAATCTGG9914401.10336252355471No Hit
ATTGTTCCAAATGCTTCTTTCTTCCTGCCCTGCAATCCTCTGGGCCATAA9830751.0940532083066512No Hit
AAGAAGTTGCTGCTTGAAAATCCGGAGGTGGAGTTCAGTAATAAACTGAA9809551.091693883940138No Hit
GACTGACAGCAGCAATCTAGGAAATGGGACAATGAGTGAGATGAAAACTG9751131.0851923872660008No Hit
CAGTGTGTGGGTCATGGTTGGACCTCTGCTTGATGTGCTTTGAGAAGAAA9067821.0091475791111786No Hit
GTAACAGAACCAATCCATTGCCAAATATGCCTGTTCATTATTTGGTGAAC9066901.0090451933367828No Hit
TGCTCAGCAACAATAGGATAAACTTATGAAAAGAAAGGAGTAGCAGTCCC9051531.0073346831710606No Hit
CCTCAATGGAGAGAGAAAATTATCTGGAAGGCCTGAGTTCTCAGAGACTG8940640.9949938586898028No Hit
ACAGGAAAACCTTACCTAGCCAAACATCTGCATGGTGATACTGTGGATGT8478010.943508281723762No Hit
AACATATTGCCACCTATCTGCTGGCTTCTTTCGTTGACATGAGGCAGTGG8399570.9347787815676627No Hit
GAGGAAACACAGGTGAACTTGGTTAGGGCACTGAGATGAGGTCTGCGTCT8305380.9242964814694603No Hit
TCATACCGCTCTAGGTCCCAGGAAAGTAGGATCAAGTAGGCACTGCACTC6999100.7789220364935618No Hit
CCAAAAAGGGAAGATTCTAGCAGCAAGGTCCCATTCTGACTCCAAGATGT6938490.7721768171608084No Hit
TTCTTTCCCTTCTGGTAATCACTTAGGGTTCTATGAGTAGCCACATGTTT5997870.6674962659518567No Hit
TTAGAGTCATAGGCCTTTCCCCCCAATCAGTCTTGAGGAAGAAGAAGAAA5889320.655415857461997No Hit
AAATGTCACACTAACTGGTGAATGTTTGAGATGGAGAACTGTTGGAGGAG5422300.6034417222898716No Hit
GCTGTTTAGCAGTCTTCACTGTTGATACCTTCAGGTGCTGGATAATCTGA5122370.5700628469479685No Hit
ACTCCTAAACTGTAAAGTTGCTGTTAGTTAGACCACCCCTGGCCCAAAGC3943460.43886326728163644No Hit
AAAACAATGGAGAAAGAAAAAAAAAGATTGTGTTTTTAGAGGATAAATTC2902960.32306718221762093No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)