FastQCFastQC Report
Fri 2 Jun 2023
EGAF00002529135

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529135
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences89856233
Sequences flagged as poor quality0
Sequence length1-144
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CCATATCATTCTTGCCTGTGTACATACATCTCTTCTCCACTGCTCAGAAC15903111.7698393833180164No Hit
CTCTGGCTTGATCCTTGTATATGAGCCCTAGCATCCAGTCCAAGTATTTC13243621.4738677059831788No Hit
GATTATGGTCGCTGAAAAATCTTTGTCAGAGGCAGTTGGGATAACCAGCC13067331.4542485884089977No Hit
CTGTGCAGGAGTACATCATTACAGCAATCAAAGAAGTACTGAGGATTCAC13054981.4528741706766186No Hit
CTCTGAGAAGCATTCCATTCTGTAAAATTTCCCGGCATGTGCATCTTATG12820471.4267758142053428No Hit
CCGTCTCTTGTATCAAATCAATTTTGGAATGAAGTGAGGATATAAACAAG12684341.4116260582613118No Hit
AGACATTTCAAAATGACCCTGTAGGCAAGGCCCAGACATAAAAGTTACAT12544021.3960100018882384No Hit
CTATAGTTGAAGATGAGATTTAGGTGGGGACACAGCCAAACAATGTAAGA12402911.3803060272958472No Hit
ATTGGTACTTGCAACACTTTCTCTGGAATTTGGCCATTTCTCTGCTCTTT12202031.3579503160342812No Hit
TAGAAGGGTCTATGCTGTGAAGATTGTTGTTTACCAGTTTTATGCACTCT12076551.3439857867177674No Hit
GTGATGTTTGGCACAATCGGATTGGTTCCCATGAGGTCCAGGGCTGCGGC11685901.3005107837093504No Hit
AGTGTGACATGCTCATAGAGACTTCCTCTTGAAGCATACAGAATGGAGAG11597121.2906305564801497No Hit
GTACGTTTGCTTTTGTGGTAATTTCTCTGGCCATGCTCTCCCTTCCCTCC10888751.2117968488618924No Hit
GGTCTAGAGTGATGGTGTCCTTCTCAAGCGTCTAAGTCCTGTCCAGGCTA10435671.1613740807496349No Hit
AACTTCGGTTGACAATGATCCTGCTCAGTGGTCTCCATCGTTTTTGCCAT10240461.1396493774672258No Hit
AAGAAGTTGCTGCTTGAAAATCCGGAGGTGGAGTTCAGTAATAAACTGAA10232651.1387802112737133No Hit
ACTGCTTCTCAACTAGGAGGATTTTTGTCCCCTCTGGGTACGGGCAATGT10050261.1184822314997336No Hit
ATTGTGTGTCATCCAAAAGACAAACAGACTGGCCGAATAGAAAGCAGATC9976841.110311401547403No Hit
AATGCTTGAAACTCCTGAACAGCCCTGGCTCCTCACACTTCCTGCCATCC9814741.092271473254393No Hit
TGCATTGGCATGTTACATGATACTGTGTATAGGAGAAAGTGATGAAGAGA9729621.0827985633450714No Hit
GCTGAAGCACAATCACTTAGTATATTTCCTTACAGTCTTCTTTCAAATGC9695261.0789746772491564No Hit
TATGGAAAATGTCAGACCCTTCCACAAAGTTAGAGTAGTGTAATGAACCA9650831.0740301120791476No Hit
GCTGTTTAGCAGTCTTCACTGTTGATGCCTTCAGGTACTGACAAATCTGG9185291.0222206844571373No Hit
ATGATGCATGGAGGAACAGGTGGGAAGGTGGCCCAGTCACAGTCCCCACC8769030.9758955730984182No Hit
GACTGACAGCAGCAATCTAGGAAATGGGACAATGAGTGAGATGAAAACTG8702790.9685237973419162No Hit
CAGTGTGTGGGTCATGGTTGGACCTCTGCTTGATGTGCTTTGAGAAGAAA8334800.9275706004724235No Hit
ATTGTTCCAAATGCTTCTTTCTTCCTGCCCTGCAATCCTCTGGGCCATAA8182670.910640222364986No Hit
TGCTCAGCAACAATAGGATAAACTTATGAAAAGAAAGGAGTAGCAGTCCC8115580.9031738510560531No Hit
ACAGGAAAACCTTACCTAGCCAAACATCTGCATGGTGATACTGTGGATGT8048790.8957408664126839No Hit
CCTCAATGGAGAGAGAAAATTATCTGGAAGGCCTGAGTTCTCAGAGACTG8017100.8922141216402872No Hit
GTAACAGAACCAATCCATTGCCAAATATGCCTGTTCATTATTTGGTGAAC7924750.8819365930908766No Hit
AACATATTGCCACCTATCTGCTGGCTTCTTTCGTTGACATGAGGCAGTGG7844440.8729989827194291No Hit
GAGGAAACACAGGTGAACTTGGTTAGGGCACTGAGATGAGGTCTGCGTCT7550630.8403011953550289No Hit
CCAAAAAGGGAAGATTCTAGCAGCAAGGTCCCATTCTGACTCCAAGATGT6254460.696051881008633No Hit
TCATACCGCTCTAGGTCCCAGGAAAGTAGGATCAAGTAGGCACTGCACTC6205510.6906042900774618No Hit
TTCTTTCCCTTCTGGTAATCACTTAGGGTTCTATGAGTAGCCACATGTTT5795160.6449368960303511No Hit
TTAGAGTCATAGGCCTTTCCCCCCAATCAGTCTTGAGGAAGAAGAAGAAA5270420.5865391664037374No Hit
AAATGTCACACTAACTGGTGAATGTTTGAGATGGAGAACTGTTGGAGGAG5137860.5717867117799162No Hit
GCTGTTTAGCAGTCTTCACTGTTGATACCTTCAGGTGCTGGATAATCTGA4730700.5264743292766345No Hit
ACTCCTAAACTGTAAAGTTGCTGTTAGTTAGACCACCCCTGGCCCAAAGC3419710.38057571365138354No Hit
AAAACAATGGAGAAAGAAAAAAAAAGATTGTGTTTTTAGAGGATAAATTC2809690.31268726789381435No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)