FastQCFastQC Report
Fri 20 Jan 2023
EGAF00002529136

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529136
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382313123
Sequences flagged as poor quality0
Sequence length1-144
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGTTGCTGAAATCTGTAGGCATTCTTCATTGAAATTTTGTTTATGCTC92422762.417462400316298No Hit
ATCCTCTGCATACCTTCTTTTTGTGAATAATATCACTTTTCCCACTTACC90047032.355321452044428No Hit
CTTAAGTGGAGAAAGGGTGTTTATCTTTCTTCATTCAAACTGTTTATTCT89085782.3301784490405786No Hit
ATCTCCTCACTCAGGTGTCTGATGGCCCTGAAGACACAGTTGTATAGGAT87411022.286372471708223No Hit
ATGTTTTACAATCTCCTCCCAGTGCTATATCACAGTCAGAACCCAAAATA85251592.229889189547909No Hit
CTGCTTACAGCTGTTGTTCTGCTGCTAAGTGGCTGTGTAAGCTTAGACAC83871232.193783706451531No Hit
ACCATCTGAAACTTGAAAGGATGATGATTTGAACTTAATTTTAGTTAGCC79183992.071181584839294No Hit
CAAAACCACTTTGTTCTCAGATCTGTCACTGTGGTTTTTGTCAGATAAGT78214932.045834299023003No Hit
TTTCTTCAGAAGAGGAGGATAATATGGTTTAGAGATTTAAAAGCCAGGTT70972991.8564099877889884No Hit
TGTGGATGATAGAGTCAGGAACCTCACAGAATTATTCCCAGTGAATAGGA70824561.8525275680897828No Hit
ATTTCCTCTAAAATGGGTCATTTCTGAAATAGACATATGTGTTTATAAAT69397691.815205542918285No Hit
TTGATCCTTTCTAATTTGCAATACATTGTCCCTGTGTTTGAGTGAGAGAT69062551.8064394300166358No Hit
CCTCTTCACCACCAACTAGTACCTCTAAATCCTTTGCAATTTCCAGAACA67597001.768105668713862No Hit
CTGTTGAAACATTGTCTTTTGTGATGTGATTGCCTCGTGCAAAAAAAATG62375801.6315369849336823No Hit
AGGAATGTATATGACTGCCAAGTACAATTACTTTTGGTCATTCGAGTTTT62238671.6279501344765506No Hit
TATGCTGGTGTCAAAAATTCAAATGCTATCTAATCTGTCACAGGAGTGGA60088651.57171298564083No Hit
AAATCTAAACAGGGCTCCTACGTCCAGCAACTCCTATTTCTATATTCCAT58899011.5405960835929768No Hit
TCTAGTTGCTGGAAAATGTGTCTCCTTATAGAAAGCAAAAATGTGCCTCT58014001.5174472574931728No Hit
TATTGCATACCTTTTCTTGCTAAGTATAAATATTCCATTGTATGAACATA57225151.496813647173707No Hit
TAAATGAATGCTGGAAGAAAGAGCTTCCTGAGTGGGAAAATAAGAAGGGG52930561.3844819028092843No Hit
ACAACTGTGAACAATTGGAAAAAGCTGCAAAACAATGTGGACATTCTTTT49101431.2843249955612954No Hit
GTTGATGGTTCTCGTCAATACTGATGATTAACCAGGATTAACGTTTTCTC48914071.2794243005882902No Hit
TAGTCTTTCTCTCTGGTTGCTGGCTCAGAGCTCTTAAAACCTCCGAATTT41419171.0833834233830366No Hit
ATGACTACATCAGGTGGTGATCTATGGAGACACTATCTTTTGTTTTTACT40035521.047191885171046No Hit
TCCAGTTCATTGGCATATAGTTGTTCATAATAGTCTTTGATGATGTTTGA39299261.027933848872878No Hit
GAGTCAGCCTCCTTTTTAAGTTTTACAGTGGGAGGTAGACTCTTACTTCT32603390.8527928558706577No Hit
GGTGTTAAAACAACAGTGGTAATCCTGGAACCCTCAAGTGACTTTGTCAT32294100.8447028903059652No Hit
GGAAAGCCAGAAACTGATATTGCCCAAAGAACCTTAGGTTTCAGGGCCAT28128770.7357521441920266No Hit
CTTTCAAATTTTCCACAGTGTTTCATATGTATTTATTGTGTAAAATACAT21240940.5555901359943639No Hit
CTTTCAAATTTTCCACAGTGTTTCATATATATTTATTGTGTAAAATACAT19062010.49859680071719636No Hit
CTTAAGTGGAGAAAGGGTGTAGGCACAAGAAACAAGTCAGAAAGACCTTG14053480.36759083469912696No Hit
AAAGTTGCATGGTGCTGGTGGGAGTGTCACTTAGCATACTTATGAATTAT12274070.3210475723063265No Hit
GAGTATCTTTCTGGGGAAAAAAAGTTTTGTTATAATTTTTTTTTTATTTT10114270.2645546122150769No Hit
AAAGTTGCATGGTGCTGGTACCAAAACAGAGATATAGATCAATGGAACAG5504290.14397334720838237No Hit
CTGGTTGCTGAAATCTGTGATGATTCTTAATCCAGGAAGACTTCTAACAC5322290.1392128514510866No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)