FastQCFastQC Report
Fri 30 Dec 2022
EGAF00002529137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382313123
Sequences flagged as poor quality0
Sequence length1-144
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGTTGCTGAAATCTGTAGGCATTCTTCATTGAAATTTTGTTTATGCTC87553022.290086704661718No Hit
CTTAAGTGGAGAAAGGGTGTTTATCTTTCTTCATTCAAACTGTTTATTCT77909202.0378374508478485No Hit
CTGCTTACAGCTGTTGTTCTGCTGCTAAGTGGCTGTGTAAGCTTAGACAC77651322.0310921945517415No Hit
ATCCTCTGCATACCTTCTTTTTGTGAATAATATCACTTTTCCCACTTACC76876202.010817713939681No Hit
ATGTTTTACAATCTCCTCCCAGTGCTATATCACAGTCAGAACCCAAAATA75023331.9623529899076995No Hit
CAAAACCACTTTGTTCTCAGATCTGTCACTGTGGTTTTTGTCAGATAAGT74222911.941416748072234No Hit
ATCTCCTCACTCAGGTGTCTGATGGCCCTGAAGACACAGTTGTATAGGAT74207421.941011582801462No Hit
ACCATCTGAAACTTGAAAGGATGATGATTTGAACTTAATTTTAGTTAGCC70650681.8479794636816587No Hit
TGTGGATGATAGAGTCAGGAACCTCACAGAATTATTCCCAGTGAATAGGA67925471.7766973172929772No Hit
TTTCTTCAGAAGAGGAGGATAATATGGTTTAGAGATTTAAAAGCCAGGTT67883301.7755942947320698No Hit
TTGATCCTTTCTAATTTGCAATACATTGTCCCTGTGTTTGAGTGAGAGAT65470411.7124813683154685No Hit
CCTCTTCACCACCAACTAGTACCTCTAAATCCTTTGCAATTTCCAGAACA60281421.5767551876580497No Hit
TATGCTGGTGTCAAAAATTCAAATGCTATCTAATCTGTCACAGGAGTGGA58908601.5408469251001882No Hit
CTGTTGAAACATTGTCTTTTGTGATGTGATTGCCTCGTGCAAAAAAAATG56649331.4817521709815857No Hit
TCTAGTTGCTGGAAAATGTGTCTCCTTATAGAAAGCAAAAATGTGCCTCT55402281.4491336202445764No Hit
AAATCTAAACAGGGCTCCTACGTCCAGCAACTCCTATTTCTATATTCCAT54456261.4243889818032742No Hit
TATTGCATACCTTTTCTTGCTAAGTATAAATATTCCATTGTATGAACATA53595081.401863466768835No Hit
ATTTCCTCTAAAATGGGTCATTTCTGAAATAGACATATGTGTTTATAAAT51815141.355306341393884No Hit
AGGAATGTATATGACTGCCAAGTACAATTACTTTTGGTCATTCGAGTTTT51313101.3421746969433743No Hit
TAAATGAATGCTGGAAGAAAGAGCTTCCTGAGTGGGAAAATAAGAAGGGG51260951.3408106318129185No Hit
ACAACTGTGAACAATTGGAAAAAGCTGCAAAACAATGTGGACATTCTTTT44419811.161869873872993No Hit
GTTGATGGTTCTCGTCAATACTGATGATTAACCAGGATTAACGTTTTCTC44049871.1521935123320368No Hit
TAGTCTTTCTCTCTGGTTGCTGGCTCAGAGCTCTTAAAACCTCCGAATTT39904601.0437674670142045No Hit
TCCAGTTCATTGGCATATAGTTGTTCATAATAGTCTTTGATGATGTTTGA37975010.9932960109245322No Hit
ATGACTACATCAGGTGGTGATCTATGGAGACACTATCTTTTGTTTTTACT31261790.8177012014311631No Hit
GAGTCAGCCTCCTTTTTAAGTTTTACAGTGGGAGGTAGACTCTTACTTCT29417150.7694517459710637No Hit
GGTGTTAAAACAACAGTGGTAATCCTGGAACCCTCAAGTGACTTTGTCAT22017460.575901235804558No Hit
CTTTCAAATTTTCCACAGTGTTTCATATGTATTTATTGTGTAAAATACAT16455340.4304152541475799No Hit
GGAAAGCCAGAAACTGATATTGCCCAAAGAACCTTAGGTTTCAGGGCCAT15646980.4092713291455601No Hit
CTTTCAAATTTTCCACAGTGTTTCATATATATTTATTGTGTAAAATACAT14794220.38696605243132076No Hit
AAAGTTGCATGGTGCTGGTGGGAGTGTCACTTAGCATACTTATGAATTAT13755110.35978649887987235No Hit
CTTAAGTGGAGAAAGGGTGTAGGCACAAGAAACAAGTCAGAAAGACCTTG12004610.31399942292851923No Hit
GAGTATCTTTCTGGGGAAAAAAAGTTTTGTTATAATTTTTTTTTTATTTT7826790.20472198125409363No Hit
AAAGTTGCATGGTGCTGGTACCAAAACAGAGATATAGATCAATGGAACAG5498740.14382817824435495No Hit
CTGGTTGCTGAAATCTGTGATGATTCTTAATCCAGGAAGACTTCTAACAC5007080.13096803899143164No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)