FastQCFastQC Report
Mon 13 May 2024
EGAF00002529137

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529137
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences382313123
Total Bases54.4 Gbp
Sequences flagged as poor quality0
Sequence length1-144
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGGTTGCTGAAATCTGTAGGCATTCTTCATTGAAATTTTGTTTATGCTC87553032.2900869662274186No Hit
CTTAAGTGGAGAAAGGGTGTTTATCTTTCTTCATTCAAACTGTTTATTCT77909212.0378377124135496No Hit
CTGCTTACAGCTGTTGTTCTGCTGCTAAGTGGCTGTGTAAGCTTAGACAC77651332.0310924561174426No Hit
ATCCTCTGCATACCTTCTTTTTGTGAATAATATCACTTTTCCCACTTACC76876222.0108182370710823No Hit
ATGTTTTACAATCTCCTCCCAGTGCTATATCACAGTCAGAACCCAAAATA75023341.9623532514734001No Hit
CAAAACCACTTTGTTCTCAGATCTGTCACTGTGGTTTTTGTCAGATAAGT74222921.941417009637935No Hit
ATCTCCTCACTCAGGTGTCTGATGGCCCTGAAGACACAGTTGTATAGGAT74207461.941012629064266No Hit
ACCATCTGAAACTTGAAAGGATGATGATTTGAACTTAATTTTAGTTAGCC70650711.8479802483787615No Hit
TGTGGATGATAGAGTCAGGAACCTCACAGAATTATTCCCAGTGAATAGGA67925491.776697840424379No Hit
TTTCTTCAGAAGAGGAGGATAATATGGTTTAGAGATTTAAAAGCCAGGTT67883321.7755948178634715No Hit
TTGATCCTTTCTAATTTGCAATACATTGTCCCTGTGTTTGAGTGAGAGAT65470461.7124826761439733No Hit
CCTCTTCACCACCAACTAGTACCTCTAAATCCTTTGCAATTTCCAGAACA60281431.5767554492237503No Hit
TATGCTGGTGTCAAAAATTCAAATGCTATCTAATCTGTCACAGGAGTGGA58908601.5408469251001882No Hit
CTGTTGAAACATTGTCTTTTGTGATGTGATTGCCTCGTGCAAAAAAAATG56649331.4817521709815857No Hit
TCTAGTTGCTGGAAAATGTGTCTCCTTATAGAAAGCAAAAATGTGCCTCT55402291.4491338818102772No Hit
AAATCTAAACAGGGCTCCTACGTCCAGCAACTCCTATTTCTATATTCCAT54456261.4243889818032742No Hit
TATTGCATACCTTTTCTTGCTAAGTATAAATATTCCATTGTATGAACATA53595121.4018645130316387No Hit
ATTTCCTCTAAAATGGGTCATTTCTGAAATAGACATATGTGTTTATAAAT51815171.355307126090987No Hit
AGGAATGTATATGACTGCCAAGTACAATTACTTTTGGTCATTCGAGTTTT51313101.3421746969433743No Hit
TAAATGAATGCTGGAAGAAAGAGCTTCCTGAGTGGGAAAATAAGAAGGGG51260981.3408114165100213No Hit
ACAACTGTGAACAATTGGAAAAAGCTGCAAAACAATGTGGACATTCTTTT44419821.1618701354386938No Hit
GTTGATGGTTCTCGTCAATACTGATGATTAACCAGGATTAACGTTTTCTC44049871.1521935123320368No Hit
TAGTCTTTCTCTCTGGTTGCTGGCTCAGAGCTCTTAAAACCTCCGAATTT39904621.0437679901456065No Hit
TCCAGTTCATTGGCATATAGTTGTTCATAATAGTCTTTGATGATGTTTGA37975040.993296795621635No Hit
ATGACTACATCAGGTGGTGATCTATGGAGACACTATCTTTTGTTTTTACT31261810.8177017245625648No Hit
GAGTCAGCCTCCTTTTTAAGTTTTACAGTGGGAGGTAGACTCTTACTTCT29417150.7694517459710637No Hit
GGTGTTAAAACAACAGTGGTAATCCTGGAACCCTCAAGTGACTTTGTCAT22017460.575901235804558No Hit
CTTTCAAATTTTCCACAGTGTTTCATATGTATTTATTGTGTAAAATACAT16455340.4304152541475799No Hit
GGAAAGCCAGAAACTGATATTGCCCAAAGAACCTTAGGTTTCAGGGCCAT15647000.40927185227696194No Hit
CTTTCAAATTTTCCACAGTGTTTCATATATATTTATTGTGTAAAATACAT14794220.38696605243132076No Hit
AAAGTTGCATGGTGCTGGTGGGAGTGTCACTTAGCATACTTATGAATTAT13755110.35978649887987235No Hit
CTTAAGTGGAGAAAGGGTGTAGGCACAAGAAACAAGTCAGAAAGACCTTG12004610.31399942292851923No Hit
GAGTATCTTTCTGGGGAAAAAAAGTTTTGTTATAATTTTTTTTTTATTTT7826790.20472198125409363No Hit
AAAGTTGCATGGTGCTGGTACCAAAACAGAGATATAGATCAATGGAACAG5498980.14383445582117776No Hit
CTGGTTGCTGAAATCTGTGATGATTCTTAATCCAGGAAGACTTCTAACAC5007090.13096830055713257No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)