FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002529138

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529138
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7431728
Sequences flagged as poor quality0
Sequence length1-144
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGCTCAGGAGGCTCAGGTGGGAGTATCGCTTGAGCCCAGGAAGTCGA3583444.821812638998629No Hit
CTACGAATGAAATACCAATTCCATTCAGAATAAGAGAAGGAAACAAATAA3446714.637831201572501No Hit
GATTGGGAGTTAGAATAAAGGTGAAAACTCATGTTTTTTTCAATACATTT3110624.185594521220367No Hit
CATCCCTCTGACATATTTGTGTATTTTTATTTGTGTATTATCTTTCTTTG3108384.182580417367266No Hit
AGCTCACATTGACCTTGGTGGGGTCTGGTTCTTCAGTGCCCCGTAGGCAC3051574.106137899557142No Hit
CAATCCCTCTAACTCCTGGTTCTTTGTCAAAAATGGACACAGTTAATGAG2988804.021675712566445No Hit
CAGTGTTCCCTACTCTAATGCTCTTTTAGAGAGACTCAAAATACATTTGT2795163.761117199122465No Hit
CATCTGTGCATTCAGAAATGCATTCATTAATTCATTGACTCAAGATGTAT2339383.1478277999410094No Hit
CCTTTATATCCCTTACACCCACTTCAAAGAAAGCTTCAAGTACAGAGAAG2193082.9509691420353383No Hit
CTCTCACCAGGGAATAGAAAGAAATTAAAAGACATGTAGTAATTGTTCAG1446281.9460884467246378No Hit
CTCTCACCAGGGAATAGAAAGAAATTAAAAGACATGTAGTAATTATTCAG1413271.9016707823537138No Hit
CTTGAAGAAGGCGAACCGTGCAGGGAAGTAGTAGCTGTGGGGTCTCACCT671200.9031546902685351No Hit
AGATGCTCAGGAGGCTCAGCCATTGCTGGTGACGTCTGCACTGCCCTGTG425630.5727200995515445No Hit
TTGCAGTCTACCAAGGGGACAAGTCTAACAACAAGCACACAACAGTACAA383430.5159365358904416No Hit
TGCTATGGTTCCCATACAATCTCACCTTGTTTCTGCATACGCTGTTGGAC379160.5101909004204674No Hit
TTCTGCCTCAGTTGAGATGACCATATGGATTTCTCAACTTTGTTTTATTA362430.487679312267618No Hit
TGCTATGGTTCCCATACAATCTCACCTTCTTTCTGCATACGCTGTTGGAC351050.4723665882282021No Hit
AGATGCTCAGGAGGCTCACCTTTGGTGAGGGCTGTCACCCTTTAGCCACA232240.3124979816268841No Hit
AGATGCTCAGGAGGCTCAGGGGCTGTGTGCCTCGCGGCCGACGCACCTGC231860.31198666043751866No Hit
AGATGCTCAGGAGGCTCAGGCAGTGAACCCAGGAGGCGGAGGTTGCAGTG130110.1750736840745517No Hit
CTTTATATCCCTTACACCCACTTCAAAGAAAGCTTCAAGTACAGAGAAGA88700.11935313025449801No Hit
TGCAGTCTACCAAGGGGACAAGTCTAACAACAAGCACACAACAGTACAAT86920.1169579941569444No Hit
CATCTGTGCATTCAGAAATGGCTCATGAAGTCAACTAAGCGGACTGTGGA79430.10687958439813729No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)