FastQCFastQC Report
Wed 8 May 2024
EGAF00002529139

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529139
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7431728
Total Bases1 Gbp
Sequences flagged as poor quality0
Sequence length1-144
%GC41

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGAATGAAATACCAATTCCATTCAGAATAAGAGAAGGAAACAAATAA2992344.026439073120006No Hit
AGATGCTCAGGAGGCTCAGGTGGGAGTATCGCTTGAGCCCAGGAAGTCGA2761023.7151790270042175No Hit
CATCCCTCTGACATATTTGTGTATTTTTATTTGTGTATTATCTTTCTTTG2741833.6893573069412664No Hit
GATTGGGAGTTAGAATAAAGGTGAAAACTCATGTTTTTTTCAATACATTT2721363.661813241819399No Hit
AGCTCACATTGACCTTGGTGGGGTCTGGTTCTTCAGTGCCCCGTAGGCAC2616483.520688593554554No Hit
CAATCCCTCTAACTCCTGGTTCTTTGTCAAAAATGGACACAGTTAATGAG2509553.376805502031291No Hit
CAGTGTTCCCTACTCTAATGCTCTTTTAGAGAGACTCAAAATACATTTGT2379643.2020009343721942No Hit
CCTTTATATCCCTTACACCCACTTCAAAGAAAGCTTCAAGTACAGAGAAG1877392.5261823360596622No Hit
CATCTGTGCATTCAGAAATGCATTCATTAATTCATTGACTCAAGATGTAT1577162.122198228998693No Hit
CTCTCACCAGGGAATAGAAAGAAATTAAAAGACATGTAGTAATTGTTCAG1168761.5726625086386368No Hit
CTCTCACCAGGGAATAGAAAGAAATTAAAAGACATGTAGTAATTATTCAG1147041.5434364659201736No Hit
CTTGAAGAAGGCGAACCGTGCAGGGAAGTAGTAGCTGTGGGGTCTCACCT745771.0034947457711048No Hit
TGCTATGGTTCCCATACAATCTCACCTTGTTTCTGCATACGCTGTTGGAC341010.4588569441723378No Hit
TGCTATGGTTCCCATACAATCTCACCTTCTTTCTGCATACGCTGTTGGAC314200.422781888680533No Hit
TTGCAGTCTACCAAGGGGACAAGTCTAACAACAAGCACACAACAGTACAA304220.4093529795493054No Hit
TTCTGCCTCAGTTGAGATGACCATATGGATTTCTCAACTTTGTTTTATTA219240.29500541462227897No Hit
AGATGCTCAGGAGGCTCAGCCATTGCTGGTGACGTCTGCACTGCCCTGTG215000.28930014661462317No Hit
AGATGCTCAGGAGGCTCACCTTTGGTGAGGGCTGTCACCCTTTAGCCACA158770.2136380664093196No Hit
AGATGCTCAGGAGGCTCAGGCAGTGAACCCAGGAGGCGGAGGTTGCAGTG118220.1590747131757244No Hit
TGCAGTCTACCAAGGGGACAAGTCTAACAACAAGCACACAACAGTACAAT117730.15841537795785854No Hit
CTTTATATCCCTTACACCCACTTCAAAGAAAGCTTCAAGTACAGAGAAGA79430.10687958439813729No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)