FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002529140

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529140
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7440156
Sequences flagged as poor quality0
Sequence length1-144
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGCTCAGGAGGCTCAGGTGGGAGTATCGCTTGAGCCCAGGAAGTCGA4050195.443689621561698No Hit
CAATCCCTCTAACTCCTGGTTCTTTGTCAAAAATGGACACAGTTAATGAG3464964.657106652064822No Hit
CTACGAATGAAATACCAATTCCATTCAGAATAAGAGAAGGAAACAAATAA3170974.26196708778687No Hit
CAGTGTTCCCTACTCTAATGCTCTTTTAGAGAGACTCAAAATACATTTGT2800473.763993658197489No Hit
AGCTCACATTGACCTTGGTGGGGTCTGGTTCTTCAGTGCCCCGTAGGCAC2789253.748913329236645No Hit
CATCCCTCTGACATATTTGTGTATTTTTATTTGTGTATTATCTTTCTTTG2314963.111440136470257No Hit
CTTGAAGAAGGCGAACCGTGCAGGGAAGTAGTAGCTGTGGGGTCTCACCT2100332.822965002346725No Hit
AGATGCTCAGGAGGCTCAGCCATTGCTGGTGACGTCTGCACTGCCCTGTG1263581.6983246050217227No Hit
TTGCAGTCTACCAAGGGGACAAGTCTAACAACAAGCACACAACAGTACAA1098971.4770792440373561No Hit
TTCTGCCTCAGTTGAGATGACCATATGGATTTCTCAACTTTGTTTTATTA901731.2119772757452933No Hit
AGATGCTCAGGAGGCTCAGGGGCTGTGTGCCTCGCGGCCGACGCACCTGC717470.9643211782118547No Hit
TGCTATGGTTCCCATACAATCTCACCTTGTTTCTGCATACGCTGTTGGAC597430.802980475140575No Hit
TGCTATGGTTCCCATACAATCTCACCTTCTTTCTGCATACGCTGTTGGAC528000.7096625393338527No Hit
AGATGCTCAGGAGGCTCACCTTTGGTGAGGGCTGTCACCCTTTAGCCACA421850.5669907996552761No Hit
AGATGCTCAGGAGGCTCAGGCAGTGAACCCAGGAGGCGGAGGTTGCAGTG328660.44173804957853036No Hit
GATTGGGAGTTAGAATAAAGGTGAAAACTCATGTTTTTTTCAATACATTT305480.4105827888555025No Hit
CCTTTATATCCCTTACACCCACTTCAAAGAAAGCTTCAAGTACAGAGAAG224710.3020232371471781No Hit
CATCTGTGCATTCAGAAATGCATTCATTAATTCATTGACTCAAGATGTAT188690.253610273763077No Hit
CATCTGTGCATTCAGAAATGGCTCATGAAGTCAACTAAGCGGACTGTGGA179220.24088204602161567No Hit
AGATGCTCAGGAGGCTCAAGGAACCTTGGTGAAGCACACTGTTTTTGAGT171180.2300758209908502No Hit
GATTGGGAGTTAGAATAAAGGTGGCACCAGAGCTTCCCTTAAAGCCAACT150390.20213285850457977No Hit
CTACGAATGAAATCCCAATTCCATTCAGAATAAGAGAAGGAAACAAATAA148950.20019741521548742No Hit
CATCCCTCTGACATCTTTGTGTATTTTTATTTGTGTATTATCTTTCTTTG122120.16413634337774638No Hit
CTCTCACCAGGGAATAGAAAGAAATTAAAAGACATGTAGTAATTATTCAG105270.1414889687796869No Hit
CTCTCACCAGGGAATAGAAAGAAATTAAAAGACATGTAGTAATTGTTCAG102720.13806162128858587No Hit
CTTGAAGAAGGCGACCCGTGCAGGGAAGTAGTAGCTGTGGGGTCTCACCT101840.13687885038969613No Hit
AGATGCTCAGGAGGCTCAGCTGGGTCTGAGTCAGGAGCAGAGGCCATCCC94900.12755108898254283No Hit
AAAGTTGCATGGTGCTGGTCATAAACTTCTGGCCTCAAGGGATCCTCCTG90800.1220404518399883No Hit
AAAGTTGCATGGTGCTGGTGCTTGGAACGGCTGGTGCCCCATTCCCTGCT88720.11924481153352161No Hit
CTTGAAGAAGGCGCACCGTGCAGGGAAGTAGTAGCTGTGGGGTCTCACCT88440.11886847533842033No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)