FastQCFastQC Report
Tue 13 Sep 2022
EGAF00002529141

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529141
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7440156
Sequences flagged as poor quality0
Sequence length1-144
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGATGCTCAGGAGGCTCAGGTGGGAGTATCGCTTGAGCCCAGGAAGTCGA3895905.2363149374824935No Hit
CTACGAATGAAATACCAATTCCATTCAGAATAAGAGAAGGAAACAAATAA3588854.823621977818745No Hit
CAATCCCTCTAACTCCTGGTTCTTTGTCAAAAATGGACACAGTTAATGAG3450554.637738778595502No Hit
CAGTGTTCCCTACTCTAATGCTCTTTTAGAGAGACTCAAAATACATTTGT2889873.884152429061971No Hit
AGCTCACATTGACCTTGGTGGGGTCTGGTTCTTCAGTGCCCCGTAGGCAC2658333.5729492768700006No Hit
CTTGAAGAAGGCGAACCGTGCAGGGAAGTAGTAGCTGTGGGGTCTCACCT2548363.4251432362439713No Hit
CATCCCTCTGACATATTTGTGTATTTTTATTTGTGTATTATCTTTCTTTG2514553.379700640685491No Hit
AGATGCTCAGGAGGCTCAGCCATTGCTGGTGACGTCTGCACTGCCCTGTG1133191.523072903310092No Hit
TTGCAGTCTACCAAGGGGACAAGTCTAACAACAAGCACACAACAGTACAA1050821.4123628590583315No Hit
TTCTGCCTCAGTTGAGATGACCATATGGATTTCTCAACTTTGTTTTATTA835311.12270495403591No Hit
AGATGCTCAGGAGGCTCAGGGGCTGTGTGCCTCGCGGCCGACGCACCTGC652970.8776294475545944No Hit
TGCTATGGTTCCCATACAATCTCACCTTGTTTCTGCATACGCTGTTGGAC629230.8457215144413638No Hit
TGCTATGGTTCCCATACAATCTCACCTTCTTTCTGCATACGCTGTTGGAC556110.7474440052063425No Hit
AGATGCTCAGGAGGCTCAGGCAGTGAACCCAGGAGGCGGAGGTTGCAGTG425450.5718294078780068No Hit
AGATGCTCAGGAGGCTCACCTTTGGTGAGGGCTGTCACCCTTTAGCCACA395850.5320452958244424No Hit
GATTGGGAGTTAGAATAAAGGTGAAAACTCATGTTTTTTTCAATACATTT308860.41512570435351087No Hit
CCTTTATATCCCTTACACCCACTTCAAAGAAAGCTTCAAGTACAGAGAAG223820.30082702566989183No Hit
CATCTGTGCATTCAGAAATGGCTCATGAAGTCAACTAAGCGGACTGTGGA179070.24068043734566855No Hit
CATCTGTGCATTCAGAAATGCATTCATTAATTCATTGACTCAAGATGTAT173350.23299242650288515No Hit
AGATGCTCAGGAGGCTCAAGGAACCTTGGTGAAGCACACTGTTTTTGAGT151490.20361132212819194No Hit
GATTGGGAGTTAGAATAAAGGTGGCACCAGAGCTTCCCTTAAAGCCAACT148710.19987484133397204No Hit
CTACGAATGAAATCCCAATTCCATTCAGAATAAGAGAAGGAAACAAATAA140590.18896109167603475No Hit
CATCCCTCTGACATCTTTGTGTATTTTTATTTGTGTATTATCTTTCTTTG118310.1590154830086896No Hit
CTTGAAGAAGGCGACCCGTGCAGGGAAGTAGTAGCTGTGGGGTCTCACCT115980.15588382824231103No Hit
CTTGAAGAAGGCGCACCGTGCAGGGAAGTAGTAGCTGTGGGGTCTCACCT110230.14815549566433822No Hit
CTCTCACCAGGGAATAGAAAGAAATTAAAAGACATGTAGTAATTATTCAG99550.13380095793690347No Hit
CTCTCACCAGGGAATAGAAAGAAATTAAAAGACATGTAGTAATTGTTCAG98900.13292732034113264No Hit
AGATGCTCAGGAGGCTCAGCTGGGTCTGAGTCAGGAGCAGAGGCCATCCC91110.12245710977027902No Hit
AAAGTTGCATGGTGCTGGTCATAAACTTCTGGCCTCAAGGGATCCTCCTG81620.10970200087202471No Hit
AAAGTTGCATGGTGCTGGTGCTTGGAACGGCTGGTGCCCCATTCCCTGCT80220.10782031989651829No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)