FastQCFastQC Report
Sun 19 May 2024
EGAF00002529142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206879991
Total Bases29.2 Gbp
Sequences flagged as poor quality0
Sequence length1-144
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATCCCTCTAACTCCTGGTTCTTTGTCAAAAATGGACACAGTTAATGAG128240266.198775405012465No Hit
AGATGCTCAGGAGGCTCAGGTGGGAGTATCGCTTGAGCCCAGGAAGTCGA127214046.149170801153022No Hit
CTACGAATGAAATACCAATTCCATTCAGAATAAGAGAAGGAAACAAATAA109413355.2887352455462935No Hit
CAGTGTTCCCTACTCTAATGCTCTTTTAGAGAGACTCAAAATACATTTGT96328424.6562463355868955No Hit
AGCTCACATTGACCTTGGTGGGGTCTGGTTCTTCAGTGCCCCGTAGGCAC71861823.473599339048695No Hit
CATCCCTCTGACATATTTGTGTATTTTTATTTGTGTATTATCTTTCTTTG68179413.295601941514006No Hit
TTCTGCCTCAGTTGAGATGACCATATGGATTTCTCAACTTTGTTTTATTA58191682.812823014865657No Hit
CTTGAAGAAGGCGAACCGTGCAGGGAAGTAGTAGCTGTGGGGTCTCACCT46306782.238340197916965No Hit
TGCTATGGTTCCCATACAATCTCACCTTGTTTCTGCATACGCTGTTGGAC35966281.7385093563736669No Hit
TTGCAGTCTACCAAGGGGACAAGTCTAACAACAAGCACACAACAGTACAA35411181.711677375314658No Hit
AGATGCTCAGGAGGCTCAGCCATTGCTGGTGACGTCTGCACTGCCCTGTG22239351.0749879624656404No Hit
AGATGCTCAGGAGGCTCACCTTTGGTGAGGGCTGTCACCCTTTAGCCACA13504860.6527871513683505No Hit
AGATGCTCAGGAGGCTCAGGGGCTGTGTGCCTCGCGGCCGACGCACCTGC11658180.563523806417799No Hit
AGATGCTCAGGAGGCTCAGGCAGTGAACCCAGGAGGCGGAGGTTGCAGTG10519630.5084894846113949No Hit
GATTGGGAGTTAGAATAAAGGTGAAAACTCATGTTTTTTTCAATACATTT5047340.24397429522316638No Hit
CATCTGTGCATTCAGAAATGGCTCATGAAGTCAACTAAGCGGACTGTGGA4202740.20314869406582678No Hit
CATCTGTGCATTCAGAAATGCATTCATTAATTCATTGACTCAAGATGTAT3847690.1859865703493771No Hit
AGATGCTCAGGAGGCTCAAGGAACCTTGGTGAAGCACACTGTTTTTGAGT3035400.1467227442019755No Hit
CCTTTATATCCCTTACACCCACTTCAAAGAAAGCTTCAAGTACAGAGAAG2775750.13417198959564922No Hit
GATTGGGAGTTAGAATAAAGGTGGCACCAGAGCTTCCCTTAAAGCCAACT2762680.1335402223601218No Hit
CGGAGGTGCCCATGAGGCTGGTGCTTTGGCAGGGAAAGGGGCCCCCCAGG2122320.10258701142344888No Hit
CTCTCACCAGGGAATAGAAAGAAATTAAAAGACATGTAGTAATTGTTCAG2069390.10002852329977141No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)