FastQCFastQC Report
Wed 25 Jan 2023
EGAF00002529142

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529142
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206879991
Sequences flagged as poor quality0
Sequence length1-144
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATCCCTCTAACTCCTGGTTCTTTGTCAAAAATGGACACAGTTAATGAG128240246.198774438268416No Hit
AGATGCTCAGGAGGCTCAGGTGGGAGTATCGCTTGAGCCCAGGAAGTCGA127214036.149170317780998No Hit
CTACGAATGAAATACCAATTCCATTCAGAATAAGAGAAGGAAACAAATAA109413345.288734762174269No Hit
CAGTGTTCCCTACTCTAATGCTCTTTTAGAGAGACTCAAAATACATTTGT96328424.6562463355868955No Hit
AGCTCACATTGACCTTGGTGGGGTCTGGTTCTTCAGTGCCCCGTAGGCAC71861773.4735969221885745No Hit
CATCCCTCTGACATATTTGTGTATTTTTATTTGTGTATTATCTTTCTTTG68179413.295601941514006No Hit
TTCTGCCTCAGTTGAGATGACCATATGGATTTCTCAACTTTGTTTTATTA58191672.812822531493633No Hit
CTTGAAGAAGGCGAACCGTGCAGGGAAGTAGTAGCTGTGGGGTCTCACCT46306772.2383397145449413No Hit
TGCTATGGTTCCCATACAATCTCACCTTGTTTCTGCATACGCTGTTGGAC35966251.7385079062575945No Hit
TTGCAGTCTACCAAGGGGACAAGTCTAACAACAAGCACACAACAGTACAA35411171.7116768919426335No Hit
AGATGCTCAGGAGGCTCAGCCATTGCTGGTGACGTCTGCACTGCCCTGTG22239351.0749879624656404No Hit
AGATGCTCAGGAGGCTCACCTTTGGTGAGGGCTGTCACCCTTTAGCCACA13504860.6527871513683505No Hit
AGATGCTCAGGAGGCTCAGGGGCTGTGTGCCTCGCGGCCGACGCACCTGC11658180.563523806417799No Hit
AGATGCTCAGGAGGCTCAGGCAGTGAACCCAGGAGGCGGAGGTTGCAGTG10519620.5084890012393707No Hit
GATTGGGAGTTAGAATAAAGGTGAAAACTCATGTTTTTTTCAATACATTT5047330.24397381185114222No Hit
CATCTGTGCATTCAGAAATGGCTCATGAAGTCAACTAAGCGGACTGTGGA4202730.20314821069380268No Hit
CATCTGTGCATTCAGAAATGCATTCATTAATTCATTGACTCAAGATGTAT3847680.18598608697735297No Hit
AGATGCTCAGGAGGCTCAAGGAACCTTGGTGAAGCACACTGTTTTTGAGT3035400.1467227442019755No Hit
CCTTTATATCCCTTACACCCACTTCAAAGAAAGCTTCAAGTACAGAGAAG2775750.13417198959564922No Hit
GATTGGGAGTTAGAATAAAGGTGGCACCAGAGCTTCCCTTAAAGCCAACT2762680.1335402223601218No Hit
CGGAGGTGCCCATGAGGCTGGTGCTTTGGCAGGGAAAGGGGCCCCCCAGG2122320.10258701142344888No Hit
CTCTCACCAGGGAATAGAAAGAAATTAAAAGACATGTAGTAATTGTTCAG2069390.10002852329977141No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)