FastQCFastQC Report
Mon 23 Jan 2023
EGAF00002529143

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529143
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences206879991
Sequences flagged as poor quality0
Sequence length1-144
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAATCCCTCTAACTCCTGGTTCTTTGTCAAAAATGGACACAGTTAATGAG126957616.136775692338463No Hit
AGATGCTCAGGAGGCTCAGGTGGGAGTATCGCTTGAGCCCAGGAAGTCGA117176135.663966313687629No Hit
CTACGAATGAAATACCAATTCCATTCAGAATAAGAGAAGGAAACAAATAA108957715.2667108826392015No Hit
CAGTGTTCCCTACTCTAATGCTCTTTTAGAGAGACTCAAAATACATTTGT95562714.619234056327854No Hit
CATCCCTCTGACATATTTGTGTATTTTTATTTGTGTATTATCTTTCTTTG68393713.305960603990939No Hit
AGCTCACATTGACCTTGGTGGGGTCTGGTTCTTCAGTGCCCCGTAGGCAC67318203.2539734594245995No Hit
TTCTGCCTCAGTTGAGATGACCATATGGATTTCTCAACTTTGTTTTATTA55397502.677760170629551No Hit
CTTGAAGAAGGCGAACCGTGCAGGGAAGTAGTAGCTGTGGGGTCTCACCT53230952.573035204743411No Hit
TGCTATGGTTCCCATACAATCTCACCTTGTTTCTGCATACGCTGTTGGAC36811561.7793678268286466No Hit
TTGCAGTCTACCAAGGGGACAAGTCTAACAACAAGCACACAACAGTACAA34308991.6584005941879607No Hit
AGATGCTCAGGAGGCTCAGCCATTGCTGGTGACGTCTGCACTGCCCTGTG18244300.8818784219688023No Hit
AGATGCTCAGGAGGCTCACCTTTGGTGAGGGCTGTCACCCTTTAGCCACA12355950.597252056144956No Hit
AGATGCTCAGGAGGCTCAGGCAGTGAACCCAGGAGGCGGAGGTTGCAGTG9888710.47799257686549296No Hit
AGATGCTCAGGAGGCTCAGGGGCTGTGTGCCTCGCGGCCGACGCACCTGC9209420.4451575986389133No Hit
GATTGGGAGTTAGAATAAAGGTGAAAACTCATGTTTTTTTCAATACATTT4899120.23680975508163088No Hit
CATCTGTGCATTCAGAAATGGCTCATGAAGTCAACTAAGCGGACTGTGGA4070350.19674933183847632No Hit
CATCTGTGCATTCAGAAATGCATTCATTAATTCATTGACTCAAGATGTAT3592300.17364173222532672No Hit
CCTTTATATCCCTTACACCCACTTCAAAGAAAGCTTCAAGTACAGAGAAG2703970.13070234520650187No Hit
GATTGGGAGTTAGAATAAAGGTGGCACCAGAGCTTCCCTTAAAGCCAACT2652500.1282144293983462No Hit
AGATGCTCAGGAGGCTCAAGGAACCTTGGTGAAGCACACTGTTTTTGAGT2433020.11760538021291773No Hit
AATCCCTCTAACTCCTGGTTCTTTGTCAAAAATGGACACAGTTAATGAGG2101080.10156032924421385No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)