FastQCFastQC Report
Fri 10 Feb 2023
EGAF00002529144

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529144
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182272061
Sequences flagged as poor quality0
Sequence length1-144
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGAATGAAATACCAATTCCATTCAGAATAAGAGAAGGAAACAAATAA83994394.608187867036847No Hit
AGATGCTCAGGAGGCTCAGGTGGGAGTATCGCTTGAGCCCAGGAAGTCGA81504034.471559138183005No Hit
AGCTCACATTGACCTTGGTGGGGTCTGGTTCTTCAGTGCCCCGTAGGCAC76169564.17889387885947No Hit
CATCCCTCTGACATATTTGTGTATTTTTATTTGTGTATTATCTTTCTTTG75499194.142115340430588No Hit
CAGTGTTCCCTACTCTAATGCTCTTTTAGAGAGACTCAAAATACATTTGT69699423.8239223069958044No Hit
CAATCCCTCTAACTCCTGGTTCTTTGTCAAAAATGGACACAGTTAATGAG68079003.735021134149572No Hit
CTTGAAGAAGGCGAACCGTGCAGGGAAGTAGTAGCTGTGGGGTCTCACCT67103973.6815280209071646No Hit
TTCTGCCTCAGTTGAGATGACCATATGGATTTCTCAACTTTGTTTTATTA45847392.5153273490444596No Hit
CATCTGTGCATTCAGAAATGCATTCATTAATTCATTGACTCAAGATGTAT45148202.4769676577037223No Hit
CCTTTATATCCCTTACACCCACTTCAAAGAAAGCTTCAAGTACAGAGAAG44646632.449450001007011No Hit
GATTGGGAGTTAGAATAAAGGTGAAAACTCATGTTTTTTTCAATACATTT43978982.412820690056278No Hit
CTCTCACCAGGGAATAGAAAGAAATTAAAAGACATGTAGTAATTGTTCAG30865851.6933944692708556No Hit
CTCTCACCAGGGAATAGAAAGAAATTAAAAGACATGTAGTAATTATTCAG29814541.6357164030750715No Hit
TGCTATGGTTCCCATACAATCTCACCTTGTTTCTGCATACGCTGTTGGAC26799051.4702774442211415No Hit
TTGCAGTCTACCAAGGGGACAAGTCTAACAACAAGCACACAACAGTACAA19856591.0893929596812977No Hit
CGGAGGTGCCCATGAGATTCCCCCACACGTGGGCCACCAGGAACTCCCCC2268110.12443541744996234No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)