FastQCFastQC Report
Fri 10 Feb 2023
EGAF00002529145

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529145
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences182272061
Sequences flagged as poor quality0
Sequence length1-144
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTACGAATGAAATACCAATTCCATTCAGAATAAGAGAAGGAAACAAATAA85970494.716602727172762No Hit
AGATGCTCAGGAGGCTCAGGTGGGAGTATCGCTTGAGCCCAGGAAGTCGA80694344.427137080542475No Hit
AGCTCACATTGACCTTGGTGGGGTCTGGTTCTTCAGTGCCCCGTAGGCAC77428844.247981812198854No Hit
CATCCCTCTGACATATTTGTGTATTTTTATTTGTGTATTATCTTTCTTTG76551584.199852658713285No Hit
CTTGAAGAAGGCGAACCGTGCAGGGAAGTAGTAGCTGTGGGGTCTCACCT71692063.933244601870168No Hit
CAGTGTTCCCTACTCTAATGCTCTTTTAGAGAGACTCAAAATACATTTGT71515293.9235464616818048No Hit
CAATCCCTCTAACTCCTGGTTCTTTGTCAAAAATGGACACAGTTAATGAG70532243.869613346831032No Hit
CCTTTATATCCCTTACACCCACTTCAAAGAAAGCTTCAAGTACAGAGAAG46181762.5336719048784992No Hit
GATTGGGAGTTAGAATAAAGGTGAAAACTCATGTTTTTTTCAATACATTT45085402.4735222585758767No Hit
TTCTGCCTCAGTTGAGATGACCATATGGATTTCTCAACTTTGTTTTATTA44548842.4440849439893038No Hit
CATCTGTGCATTCAGAAATGCATTCATTAATTCATTGACTCAAGATGTAT44346452.4329812126280834No Hit
CTCTCACCAGGGAATAGAAAGAAATTAAAAGACATGTAGTAATTGTTCAG31055361.703791564632607No Hit
CTCTCACCAGGGAATAGAAAGAAATTAAAAGACATGTAGTAATTATTCAG30027891.6474214333923618No Hit
TGCTATGGTTCCCATACAATCTCACCTTGTTTCTGCATACGCTGTTGGAC27620311.515334267274237No Hit
TTGCAGTCTACCAAGGGGACAAGTCTAACAACAAGCACACAACAGTACAA19405761.0646590538085814No Hit
CGGAGGTGCCCATGAGATTCCCCCACACGTGGGCCACCAGGAACTCCCCC2438210.13376762113860116No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)