FastQCFastQC Report
Wed 8 May 2024
EGAF00002529146

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529146
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2901556
Total Bases409.6 Mbp
Sequences flagged as poor quality0
Sequence length1-144
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTGTCTTTGGTTTCTTAAGCCAGCTAAAACTTTAGGCAGGCTTAGTT2220687.653410790624066No Hit
CACAATCAAAGGCTAACTTGTGAAAATTTTATTCTTCTACCTTACAGAAT1725995.948497978326111No Hit
CAAGTGTGGCTGATTTTAATTGATTTCTGCTTGGTACACATTTACTGATG1686845.813570373964866No Hit
ACCTAGGTGATCCTTTCAACTCCCAGGAATCAGCAATTCCATAACTAAGG1367914.714401514222024No Hit
GTGGACATGTTCTATTCTGCCGCCAGCCACTCCTGCCCTTGCCCAATGGA1233994.252856053786314No Hit
TACAGGAGCTGAAATTAGGAACATGAAAGAAACTTGAAGAGAGAAGACAT1021313.5198700283571984No Hit
GAGAGGGATAGTTCTTCCAAGCTCCACAAAGCCCCAAGATATAAGAGTAT1006873.470103627157291No Hit
TGTGCTTTGTAAAATGTGTGGCAGTATCCCTGGACTCTACCACCAGATGC870553.0002867426994344No Hit
CAAAGGACCACAACTGTGTAGCTCACACTTAGCTGGCTCAATGAATATCA751482.5899207184007476No Hit
AAAACTTTGCTAATCCTCAAACTTAAAAGTGATTCAACTTTAGGAATAGC652952.2503442980249218No Hit
TTTCTTTACCTGTCACCACACATTCAGGAGTTCCTGATTCATCTCTTAGA567831.956984459372833No Hit
TCCTTTGGAGTAATGTTAAGCATATACTGGCACCCACAGTAATATTTTAA339581.1703375706000505No Hit
CG87290.3008385845387785No Hit
TTCTTTACCTGTCACCACACATTCAGGAGTTCCTGATTCATCTCTTAGAA48920.16859919298472956No Hit
GG41340.1424752787814538No Hit
G39550.13630617503160375No Hit
CAGGAGCTGAAATTAGGAACATGAAAGAAACTTGAAGAGAGAAGACATTC32240.11111279603081932No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)