FastQCFastQC Report
Sun 11 Sep 2022
EGAF00002529147

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529147
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2901556
Sequences flagged as poor quality0
Sequence length1-144
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTGTCTTTGGTTTCTTAAGCCAGCTAAAACTTTAGGCAGGCTTAGTT1946546.708607381694512No Hit
CACAATCAAAGGCTAACTTGTGAAAATTTTATTCTTCTACCTTACAGAAT1581895.451867894329801No Hit
CAAGTGTGGCTGATTTTAATTGATTTCTGCTTGGTACACATTTACTGATG1379804.755379527398403No Hit
ACCTAGGTGATCCTTTCAACTCCCAGGAATCAGCAATTCCATAACTAAGG1327194.574063019979625No Hit
GTGGACATGTTCTATTCTGCCGCCAGCCACTCCTGCCCTTGCCCAATGGA1076673.710664209134685No Hit
GAGAGGGATAGTTCTTCCAAGCTCCACAAAGCCCCAAGATATAAGAGTAT923753.1836366418569897No Hit
TACAGGAGCTGAAATTAGGAACATGAAAGAAACTTGAAGAGAGAAGACAT885763.0527068924397804No Hit
CAAAGGACCACAACTGTGTAGCTCACACTTAGCTGGCTCAATGAATATCA693452.3899245783986247No Hit
TGTGCTTTGTAAAATGTGTGGCAGTATCCCTGGACTCTACCACCAGATGC690832.380894940507783No Hit
AAAACTTTGCTAATCCTCAAACTTAAAAGTGATTCAACTTTAGGAATAGC625542.15587774283867No Hit
TTTCTTTACCTGTCACCACACATTCAGGAGTTCCTGATTCATCTCTTAGA502851.7330356539732477No Hit
TCCTTTGGAGTAATGTTAAGCATATACTGGCACCCACAGTAATATTTTAA295711.019142832328585No Hit
NN128810.4439342201218932No Hit
TTCTTTACCTGTCACCACACATTCAGGAGTTCCTGATTCATCTCTTAGAA45020.15515812894874337No Hit
N39820.1372367102340951No Hit
CAGGAGCTGAAATTAGGAACATGAAAGAAACTTGAAGAGAGAAGACATTC31110.10721833388705922No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)