FastQCFastQC Report
Wed 8 May 2024
EGAF00002529149

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529149
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7942874
Total Bases1.1 Gbp
Sequences flagged as poor quality0
Sequence length1-144
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CAAAGGACCACAACTGTGTAGCTCACACTTAGCTGGCTCAATGAATATCA4990536.2830280324225205No Hit
AGGCTGTCTTTGGTTTCTTAAGCCAGCTAAAACTTTAGGCAGGCTTAGTT4969376.256387801191357No Hit
AAAACTTTGCTAATCCTCAAACTTAAAAGTGATTCAACTTTAGGAATAGC4581035.767471572632274No Hit
ACCTAGGTGATCCTTTCAACTCCCAGGAATCAGCAATTCCATAACTAAGG3503934.411413299518538No Hit
CACAATCAAAGGCTAACTTGTGAAAATTTTATTCTTCTACCTTACAGAAT3087883.8876104543519134No Hit
GTGGACATGTTCTATTCTGCCGCCAGCCACTCCTGCCCTTGCCCAATGGA2977463.748592763777947No Hit
GAGAGGGATAGTTCTTCCAAGCTCCACAAAGCCCCAAGATATAAGAGTAT2931293.690465189300497No Hit
CAAGTGTGGCTGATTTTAATTGATTTCTGCTTGGTACACATTTACTGATG2103812.6486760333853967No Hit
TACAGGAGCTGAAATTAGGAACATGAAAGAAACTTGAAGAGAGAAGACAT1275141.6053886792110765No Hit
TGTGCTTTGTAAAATGTGTGGCAGTATCCCTGGACTCTACCACCAGATGC642030.808309435602277No Hit
TGGATTTTCTATTTGGAATATTTTAAAAAGAATCAAATCAAAACAAAATA634730.7991188076255521No Hit
TTCTTTACCTGTCACCACACATTCAGGAGTTCCTGATTCATCTCTTAGAA407100.5125348834691322No Hit
TCCTTTGGAGTAATGTTAAGCATATACTGGCACCCACAGTAATATTTTAA333200.4194955125814661No Hit
TTTCTTTACCTGTCACCACACATTCAGGAGTTCCTGATTCATCTCTTAGA317450.39966641797414887No Hit
ACAGGAGCTGAAATTAGGAACATGAAAGAAACTTGAAGAGAGAAGACATT312640.393610675430581No Hit
AAAGGACCACAACTGTGTAGCTCACACTTAGCTGGCTCAATGAATATCAA201810.2540767989017577No Hit
NN167240.21055350997636368No Hit
ACAATCAAAGGCTAACTTGTGAAAATTTTATTCTTCTACCTTACAGAATA136460.1718017936580638No Hit
AAGTGTGGCTGATTTTAATTGATTTCTGCTTGGTACACATTTACTGATGA121930.15350866701398008No Hit
GTGCTTTGTAAAATGTGTGGCAGTATCCCTGGACTCTACCACCAGATGCC106930.13462381500701132No Hit
N86060.10834869091464877No Hit
CAGGAGCTGAAATTAGGAACATGAAAGAAACTTGAAGAGAGAAGACATTC84290.10612027837782648No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)