FastQCFastQC Report
Sun 19 May 2024
EGAF00002529151

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529151
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences191340330
Total Bases27.2 Gbp
Sequences flagged as poor quality0
Sequence length1-144
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGTTGTCTAAGTATCCTGGGCATAGCAACTAAACTTTTGTATATTTCTTC105189705.497518479245855No Hit
CTAAACCCTTAACCCTGCTACCCTAATTCCCAGTACCCAAGATCCCCTAA91968044.8065162216454835No Hit
TACTCTAAAAACATTGCCCAAAGTAGGGAAATTCGGGTTTAGTATTTTTT86073584.4984546645236785No Hit
AAGTAAGATATGGGCAGGAATTTACGTTTGCTTTTTGTTATCTTTCAAGT79612524.1607809498394825No Hit
CCCATCAATTTCACAGTAAAGAAGCTGAGGCCCAGAGAGGTGAGGAGATT77380804.044144796865355No Hit
TAGAACTGTCTTGGACATCGTGACCAAATAGACAAAATCTGTTTGAAATG69250423.619227582601117No Hit
AATTGCTATTGCTCAGTTTGGGATTCACTAAGAATTGCAACACTATATCC66483903.474641232196056No Hit
ATTTTCCAAATGCTAAACATCATATTTATGGATTATTCAGGGTTATATTA51600282.696780129939151No Hit
AATGAAAAGTCCTCTGCAGTTGGACTTTACTTGTTATTTTCCACAGATTT44529312.327230751614153No Hit
ATTTTTAGGCAAAATCTTCCTTTTGCTTGAATAAATGATACAAGAGGCAG41182052.1522932462800703No Hit
GGATGCGTACAATTCTTAAATCACTTCCCTGGCCAAAGAAACTCTAGCCC37079001.937855965859367No Hit
TCACAGGAAAGCTAGATTTGATTGCTCTTTCAGGAAAGCTGGTGGGAGCT29931941.5643299036852294No Hit
GACCGCGACAACAACAAGTGTGCCTTCTGTACCCACAGGGGCAAGTACTG23235511.2143550708833835No Hit
GAAAATGGTTCCCTTTTTATTTTATTTAAGAGTGAGATGGAAGCTGCATA11469430.5994256412121794No Hit
AATGAAAAGTCCTCTGCAGTCCACCACCGAGCCGCCGGTTCCTATTCTGT6092130.31839236401442395No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)