FastQCFastQC Report
Mon 6 Feb 2023
EGAF00002529152

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529152
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201364763
Sequences flagged as poor quality0
Sequence length1-144
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTGTCTTTGGTTTCTTAAGCCAGCTAAAACTTTAGGCAGGCTTAGTT126092656.261902436227136No Hit
CACAATCAAAGGCTAACTTGTGAAAATTTTATTCTTCTACCTTACAGAAT114098765.666272405366176No Hit
CAAGTGTGGCTGATTTTAATTGATTTCTGCTTGGTACACATTTACTGATG110553415.49020634757234No Hit
ACCTAGGTGATCCTTTCAACTCCCAGGAATCAGCAATTCCATAACTAAGG109511315.438454492656195No Hit
TACAGGAGCTGAAATTAGGAACATGAAAGAAACTTGAAGAGAGAAGACAT85063994.22437315907153No Hit
GAGAGGGATAGTTCTTCCAAGCTCCACAAAGCCCCAAGATATAAGAGTAT64523813.2043247804979664No Hit
TTTCTTTACCTGTCACCACACATTCAGGAGTTCCTGATTCATCTCTTAGA64512833.2037795013817787No Hit
TGTGCTTTGTAAAATGTGTGGCAGTATCCCTGGACTCTACCACCAGATGC61195783.0390510776704263No Hit
GTGGACATGTTCTATTCTGCCGCCAGCCACTCCTGCCCTTGCCCAATGGA58909102.925491983917762No Hit
AAAACTTTGCTAATCCTCAAACTTAAAAGTGATTCAACTTTAGGAATAGC53763462.669953729690035No Hit
CAAAGGACCACAACTGTGTAGCTCACACTTAGCTGGCTCAATGAATATCA45489612.259065057971439No Hit
TCCTTTGGAGTAATGTTAAGCATATACTGGCACCCACAGTAATATTTTAA23089551.1466529523837297No Hit
TGGATTTTCTATTTGGAATATTTTAAAAAGAATCAAATCAAAACAAAATA9095200.4516778340210397No Hit
GTGGACATGTTCTATTCTGCCATTATTCAGATGAGGCTCTCCAGGCACAG2311640.11479863535011833No Hit
CAGGAGCTGAAATTAGGAACATGAAAGAAACTTGAAGAGAGAAGACATTC2142890.10641832106444561No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)