FastQCFastQC Report
Tue 24 Jan 2023
EGAF00002529153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201364763
Sequences flagged as poor quality0
Sequence length1-144
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTGTCTTTGGTTTCTTAAGCCAGCTAAAACTTTAGGCAGGCTTAGTT119763015.947565413915045No Hit
CAAGTGTGGCTGATTTTAATTGATTTCTGCTTGGTACACATTTACTGATG109091555.417608740214394No Hit
ACCTAGGTGATCCTTTCAACTCCCAGGAATCAGCAATTCCATAACTAAGG103484215.139141946101066No Hit
CACAATCAAAGGCTAACTTGTGAAAATTTTATTCTTCTACCTTACAGAAT102533105.091908756647755No Hit
TACAGGAGCTGAAATTAGGAACATGAAAGAAACTTGAAGAGAGAAGACAT82874904.115660494184874No Hit
TTTCTTTACCTGTCACCACACATTCAGGAGTTCCTGATTCATCTCTTAGA62365043.0971178408210376No Hit
GAGAGGGATAGTTCTTCCAAGCTCCACAAAGCCCCAAGATATAAGAGTAT62171373.0874999713827784No Hit
TGTGCTTTGTAAAATGTGTGGCAGTATCCCTGGACTCTACCACCAGATGC56430772.8024153361926585No Hit
GTGGACATGTTCTATTCTGCCGCCAGCCACTCCTGCCCTTGCCCAATGGA53680062.6658119921408496No Hit
AAAACTTTGCTAATCCTCAAACTTAAAAGTGATTCAACTTTAGGAATAGC52594822.6119177564348734No Hit
CAAAGGACCACAACTGTGTAGCTCACACTTAGCTGGCTCAATGAATATCA43448912.157721606932788No Hit
TCCTTTGGAGTAATGTTAAGCATATACTGGCACCCACAGTAATATTTTAA22729281.1287615400714375No Hit
TGGATTTTCTATTTGGAATATTTTAAAAAGAATCAAATCAAAACAAAATA9120930.4529556146821974No Hit
CAGGAGCTGAAATTAGGAACATGAAAGAAACTTGAAGAGAGAAGACATTC2131960.1058755250043425No Hit
GTGGACATGTTCTATTCTGCCATTATTCAGATGAGGCTCTCCAGGCACAG2111280.10484853300773384No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)