FastQCFastQC Report
Sun 19 May 2024
EGAF00002529153

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529153
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences201364763
Total Bases28.6 Gbp
Sequences flagged as poor quality0
Sequence length1-144
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGCTGTCTTTGGTTTCTTAAGCCAGCTAAAACTTTAGGCAGGCTTAGTT119763125.947570876638431No Hit
CAAGTGTGGCTGATTTTAATTGATTTCTGCTTGGTACACATTTACTGATG109091585.417610230048045No Hit
ACCTAGGTGATCCTTTCAACTCCCAGGAATCAGCAATTCCATAACTAAGG103484395.13915088510297No Hit
CACAATCAAAGGCTAACTTGTGAAAATTTTATTCTTCTACCTTACAGAAT102533155.09191123970384No Hit
TACAGGAGCTGAAATTAGGAACATGAAAGAAACTTGAAGAGAGAAGACAT82874984.115664467074609No Hit
TTTCTTTACCTGTCACCACACATTCAGGAGTTCCTGATTCATCTCTTAGA62365163.097123800155641No Hit
GAGAGGGATAGTTCTTCCAAGCTCCACAAAGCCCCAAGATATAAGAGTAT62171373.0874999713827784No Hit
TGTGCTTTGTAAAATGTGTGGCAGTATCCCTGGACTCTACCACCAGATGC56430832.80241831585996No Hit
GTGGACATGTTCTATTCTGCCGCCAGCCACTCCTGCCCTTGCCCAATGGA53680092.6658134819745003No Hit
AAAACTTTGCTAATCCTCAAACTTAAAAGTGATTCAACTTTAGGAATAGC52595032.611928185270429No Hit
CAAAGGACCACAACTGTGTAGCTCACACTTAGCTGGCTCAATGAATATCA43449022.157727069656174No Hit
TCCTTTGGAGTAATGTTAAGCATATACTGGCACCCACAGTAATATTTTAA22729351.1287650163499559No Hit
TGGATTTTCTATTTGGAATATTTTAAAAAGAATCAAATCAAAACAAAATA9120950.45295660790463127No Hit
CAGGAGCTGAAATTAGGAACATGAAAGAAACTTGAAGAGAGAAGACATTC2131960.1058755250043425No Hit
GTGGACATGTTCTATTCTGCCATTATTCAGATGAGGCTCTCCAGGCACAG2111280.10484853300773384No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)