FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002529154

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529154
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8208471
Sequences flagged as poor quality0
Sequence length2-144
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTTATTCTTCTCTCCTGGCCCCCTGCTTCCTCATTAGCATTCCTAAGTG2457362.993687862209661No Hit
AGGAAATGGTAATATCTCAGGTTTTCCTTATAAAGTTGACATTACCTAGG2258082.750914268930231No Hit
AGATTTCAAAGAACTTGGGTCATTTCCCCATCCATGCCAATTCTTGGGTT2158312.6293690993121617No Hit
ATAAAGTCTGGTTGGAAGACAGCCATGCTTATTTGGCTGCTTTCACAGCG1943512.3676882089246587No Hit
GACTTTTTTGGGTCCTAACAGAGAACATTTGGTATGAGAATGTTTCTTGA1937642.360537059825149No Hit
AACACAGAGTTTGTCACACTGAGAAAAATAAAAAACAAGAGACAAGCATG1874072.283092673410188No Hit
ATGAAAAGTCTGTGTAGGCAAATCGGAAGGCCAAAACAAAGGAGCAAAAT1818162.2149801101812994No Hit
ATATTCCAGAAGCAGTGCTCAAGATTTTGAACCAATTAGAGATTTGGGCT1700442.0715672870136226No Hit
ACTACCACCACTGTCAAGATATAGATCATTTCTGCCACTCCAGAGAATAC1628711.9841819505727682No Hit
TTCTCATCACACTTGCTCATCTGTTCATGTGGTGCACACCTGTACAAATT1552541.8913875677942944No Hit
CTGCCTCATATCCATATCAATACCACCACTTCACAGACACACACACACAC1462451.781635093795178No Hit
TGCTTACTGAACTGTGTTCTTTGTAGAGACAGTTTGTATCTGGGTATAAA1381711.683273291700732No Hit
AACCAGAGGAAGTAGAAAGAATGAAATAATAAAAATAAAAACAAAATATA1349531.6440698882897924No Hit
GAAGAGTTTTTTCCACCAAGATCCCAGAAGACAATCTCTTTGTTATCTAT1305921.5909418453205233No Hit
TAATTTACTCTCCCCCGTAAGGACCATCAGAAAGAAAAGACTAATGCCTC1233851.5031423026285895No Hit
AGACAACCCCATTTTATGAGTTGCTCAGGACCTAAACTCCATCATCACAC1223641.4907039325594256No Hit
TGTATCACAAATGAATGCTGCTGGTTTATAAAAGAGAGAATGACCAAGTC1116651.360362971374328No Hit
AATTGACTCACTTCTGAACGTTAGGGTCTTCAAAATGATATGTATATACT1085561.3224874644742No Hit
AACGGGGTGAACATCTAGAAAAAAAGAGCATGTGACCTATAAGGAAAAGA1032801.2582124003361892No Hit
GAGAAAGAGGGAGTCATCCAGGTATGTGCCTTGGAAGTTTGAGCCACTTG953051.1610566693845907No Hit
ATCTCCAAAATTTGTGAATACATGACCTTATATCACAAAAGATACTTTGC893161.0880954565107193No Hit
AAGTTTCAGGTGATTTCTGAGAATGCTTTTTCTCTTCTGAGTGTACCAAA821001.0001862709876175No Hit
TCCAAACAGGAGTTTTGAAGAAGTCTTAGAGGAGTTCAGAACTTCAGTAG512580.6244524711118551No Hit
GTAGTGGGGAAATAAAGGAAAATAAGAATGGGAAGAAAGTCCTAATACAT509370.6205418768001982No Hit
AGATACTATCACCATGACAGTTTCAACCCACAACAAAACTTATGCACAGA492210.5996366436575095No Hit
AGATACAAAGAACCTTTTGTAGTCCAACCAAATTAGGCAGGGAAGAATGG435960.5311098741775417No Hit
CTCTCTCTCTCTCACATCCATCCTATTGTTTTTCAATGTAATTCACAATT412590.5026392856842645No Hit
GTGTTGCACACACCAAATATTTATAGCCTATCATGTACCAGAGTGATGGA319220.3888909396159163No Hit
TGTATCACAAATGAATGCTGCTGAAATCATAGGATTGATTTTGGGGCCTT216600.2638737470108623No Hit
GCTCAAAAATGGTTGTGTCGTTGATGCCGCCTGCGTGCTGCAGTACCCCT160620.19567590602439844No Hit
AGATACTATCACCATGACAGTTTCAACCCACAACAAAATTTATGCACAGA123990.15105127373904348No Hit
CTCTCTCTCTCTCACATCCATCCTATTGTTTTTCAATGTAATTCACAGTT97460.11873100361809161No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)