FastQCFastQC Report
Mon 5 Sep 2022
EGAF00002529155

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529155
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences8208471
Sequences flagged as poor quality0
Sequence length2-144
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
AGGAAATGGTAATATCTCAGGTTTTCCTTATAAAGTTGACATTACCTAGG2238872.7275116157442723No Hit
ATTTATTCTTCTCTCCTGGCCCCCTGCTTCCTCATTAGCATTCCTAAGTG2126412.5905068069315225No Hit
AGATTTCAAAGAACTTGGGTCATTTCCCCATCCATGCCAATTCTTGGGTT1968022.3975476066127297No Hit
ATAAAGTCTGGTTGGAAGACAGCCATGCTTATTTGGCTGCTTTCACAGCG1791792.1828547606490902No Hit
GACTTTTTTGGGTCCTAACAGAGAACATTTGGTATGAGAATGTTTCTTGA1788542.178895436190248No Hit
AACACAGAGTTTGTCACACTGAGAAAAATAAAAAACAAGAGACAAGCATG1713472.0874411324593827No Hit
ATGAAAAGTCTGTGTAGGCAAATCGGAAGGCCAAAACAAAGGAGCAAAAT1675282.040916024433783No Hit
ATATTCCAGAAGCAGTGCTCAAGATTTTGAACCAATTAGAGATTTGGGCT1556931.8967357014479311No Hit
ACTACCACCACTGTCAAGATATAGATCATTTCTGCCACTCCAGAGAATAC1484541.8085463175785113No Hit
TTCTCATCACACTTGCTCATCTGTTCATGTGGTGCACACCTGTACAAATT1386361.688938171311076No Hit
AACCAGAGGAAGTAGAAAGAATGAAATAATAAAAATAAAAACAAAATATA1297901.5811714508097794No Hit
GAAGAGTTTTTTCCACCAAGATCCCAGAAGACAATCTCTTTGTTATCTAT1262521.5380696356239791No Hit
TGCTTACTGAACTGTGTTCTTTGTAGAGACAGTTTGTATCTGGGTATAAA1258551.533233168515793No Hit
CTGCCTCATATCCATATCAATACCACCACTTCACAGACACACACACACAC1212291.4768767532954676No Hit
AGACAACCCCATTTTATGAGTTGCTCAGGACCTAAACTCCATCATCACAC1168441.4234563294430838No Hit
TGTATCACAAATGAATGCTGCTGGTTTATAAAAGAGAGAATGACCAAGTC1083221.3196367508638331No Hit
TAATTTACTCTCCCCCGTAAGGACCATCAGAAAGAAAAGACTAATGCCTC1024481.2480765297215524No Hit
AATTGACTCACTTCTGAACGTTAGGGTCTTCAAAATGATATGTATATACT983191.197774835289057No Hit
AACGGGGTGAACATCTAGAAAAAAAGAGCATGTGACCTATAAGGAAAAGA960821.1705225004754234No Hit
GAGAAAGAGGGAGTCATCCAGGTATGTGCCTTGGAAGTTTGAGCCACTTG891051.0855249412466708No Hit
ATCTCCAAAATTTGTGAATACATGACCTTATATCACAAAAGATACTTTGC842471.0263421774895716No Hit
AAGTTTCAGGTGATTTCTGAGAATGCTTTTTCTCTTCTGAGTGTACCAAA816080.9941924628837697No Hit
AGATACTATCACCATGACAGTTTCAACCCACAACAAAACTTATGCACAGA497330.605874102497286No Hit
GTAGTGGGGAAATAAAGGAAAATAAGAATGGGAAGAAAGTCCTAATACAT487170.5934966451121043No Hit
TCCAAACAGGAGTTTTGAAGAAGTCTTAGAGGAGTTCAGAACTTCAGTAG462000.5628331999954681No Hit
AGATACAAAGAACCTTTTGTAGTCCAACCAAATTAGGCAGGGAAGAATGG425170.5179649169741843No Hit
GTGTTGCACACACCAAATATTTATAGCCTATCATGTACCAGAGTGATGGA302380.3683755476507135No Hit
TGTATCACAAATGAATGCTGCTGAAATCATAGGATTGATTTTGGGGCCTT192730.2347940316777631No Hit
GCTCAAAAATGGTTGTGTCGTTGATGCCGCCTGCGTGCTGCAGTACCCCT129590.15787349434504916No Hit
AGATACTATCACCATGACAGTTTCAACCCACAACAAAATTTATGCACAGA124360.1515020276005117No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)