FastQCFastQC Report
Tue 26 Sep 2023
EGAF00002529159

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529159
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50621134
Sequences flagged as poor quality0
Sequence length1-144
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCCAGTTGTAATTTTGTGAGCACCTAGTGAATCTAGAAGCAGAGCGAT14596332.883445874602493No Hit
TAGAAGGGTCTATGCTGTGAAGATTGTTGTTTACCAGTTTTATGCACTCT14450542.8546456505695823No Hit
CTCTGGCTTGATCCTTGTATATGAGCCCTAGCATCCAGTCCAAGTATTTC13454352.6578523507592697No Hit
ATCTTCCAGAGATGCTGTCTGCATTAGCAAGCAAAATGTGCATATTTTTG13322182.6317427025637157No Hit
GGTCTAGAGTGATGGTGTCCTTCTCAAGCGTCTAAGTCCTGTCCAGGCTA13187212.6050799257084996No Hit
TATGGAAAATGTCAGACCCTTCCACAAAGTTAGAGTAGTGTAATGAACCA12664402.501800927652075No Hit
GTGATGTTTGGCACAATCGGATTGGTTCCCATGAGGTCCAGGGCTGCGGC12654402.499825468153282No Hit
AACTTCGGTTGACAATGATCCTGCTCAGTGGTCTCCATCGTTTTTGCCAT12178402.4057935960107097No Hit
CCGTCTCTTGTATCAAATCAATTTTGGAATGAAGTGAGGATATAAACAAG11902452.3512807911415026No Hit
ATTGGTACTTGCAACACTTTCTCTGGAATTTGGCCATTTCTCTGCTCTTT11486682.2691471115601636No Hit
AAGAAGTTGCTGCTTGAAAATCCGGAGGTGGAGTTCAGTAATAAACTGAA11275202.227370094079678No Hit
AGTGTGACATGCTCATAGAGACTTCCTCTTGAAGCATACAGAATGGAGAG11179042.20837407553928No Hit
AGACATTTCAAAATGACCCTGTAGGCAAGGCCCAGACATAAAAGTTACAT10747052.123036200650898No Hit
CTCTGAGAAGCATTCCATTCTGTAAAATTTCCCGGCATGTGCATCTTATG10654592.1047711021250533No Hit
GTGATGCTTCTGCTGATTACCTGCTCCTCTGTGCACTCATGGTCACTTCT10400022.0544818296642666No Hit
TATTCCTACTGTTGCTGCTGTTGTTATTTTCATGGTCATCCCACAGCCTC10016571.9787328351830284No Hit
GTACGTTTGCTTTTGTGGTAATTTCTCTGGCCATGCTCTCCCTTCCCTCC9791561.9342830210006754No Hit
ATTGTGTGTCATCCAAAAGACAAACAGACTGGCCGAATAGAAAGCAGATC9532851.883175908307388No Hit
GCTGAAGCACAATCACTTAGTATATTTCCTTACAGTCTTCTTTCAAATGC8243531.6284759642089408No Hit
CTATGAATGTATTCTCCATCTAAAACTCAAGTGACTCTTAGAAAGGGATC3673620.7257087523957878No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)