FastQCFastQC Report
Wed 22 Mar 2023
EGAF00002529162

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00002529162
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70960238
Sequences flagged as poor quality0
Sequence length1-144
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATTATGGTCGCTGAAAAATCTTTGTCAGAGGCAGTTGGGATAACCAGCC17090052.4083980665341063No Hit
CCGTCTCTTGTATCAAATCAATTTTGGAATGAAGTGAGGATATAAACAAG16880392.3788519424075214No Hit
CCATATCATTCTTGCCTGTGTACATACATCTCTTCTCCACTGCTCAGAAC16449302.31810101877054No Hit
GTACGTTTGCTTTTGTGGTAATTTCTCTGGCCATGCTCTCCCTTCCCTCC16413712.3130855339013943No Hit
AGACATTTCAAAATGACCCTGTAGGCAAGGCCCAGACATAAAAGTTACAT16224702.28644949020605No Hit
CTGTGCAGGAGTACATCATTACAGCAATCAAAGAAGTACTGAGGATTCAC15574922.1948798987962808No Hit
ACTGCTTCTCAACTAGGAGGATTTTTGTCCCCTCTGGGTACGGGCAATGT15070512.1237964280784967No Hit
GTGATGTTTGGCACAATCGGATTGGTTCCCATGAGGTCCAGGGCTGCGGC12815561.8060198727067402No Hit
GTAACAGAACCAATCCATTGCCAAATATGCCTGTTCATTATTTGGTGAAC12511711.7632001177899093No Hit
ATTGTGTGTCATCCAAAAGACAAACAGACTGGCCGAATAGAAAGCAGATC12415791.7496826884938013No Hit
CTCTGAGAAGCATTCCATTCTGTAAAATTTCCCGGCATGTGCATCTTATG12189131.7177408565061465No Hit
ATTGTTCCAAATGCTTCTTTCTTCCTGCCCTGCAATCCTCTGGGCCATAA12082791.7027549992151942No Hit
GCTGTTTAGCAGTCTTCACTGTTGATGCCTTCAGGTACTGACAAATCTGG12029851.6952944830878385No Hit
GACTGACAGCAGCAATCTAGGAAATGGGACAATGAGTGAGATGAAAACTG11149981.5712996903984453No Hit
CTATAGTTGAAGATGAGATTTAGGTGGGGACACAGCCAAACAATGTAAGA11068941.5598792101007328No Hit
AACTTCGGTTGACAATGATCCTGCTCAGTGGTCTCCATCGTTTTTGCCAT11002971.5505824543598627No Hit
CCTCAATGGAGAGAGAAAATTATCTGGAAGGCCTGAGTTCTCAGAGACTG9940401.4008408483635582No Hit
ATGATGCATGGAGGAACAGGTGGGAAGGTGGCCCAGTCACAGTCCCCACC9486881.336928999589883No Hit
AATGCTTGAAACTCCTGAACAGCCCTGGCTCCTCACACTTCCTGCCATCC9267321.3059877279442045No Hit
ACAGGAAAACCTTACCTAGCCAAACATCTGCATGGTGATACTGTGGATGT9225771.3001323360837658No Hit
AAGAAGTTGCTGCTTGAAAATCCGGAGGTGGAGTTCAGTAATAAACTGAA9051181.2755284163505765No Hit
TGCTCAGCAACAATAGGATAAACTTATGAAAAGAAAGGAGTAGCAGTCCC8462991.192638333597472No Hit
TTAGAGTCATAGGCCTTTCCCCCCAATCAGTCTTGAGGAAGAAGAAGAAA7926031.1169677869456975No Hit
AACATATTGCCACCTATCTGCTGGCTTCTTTCGTTGACATGAGGCAGTGG7899241.113192433204635No Hit
TTCTTTCCCTTCTGGTAATCACTTAGGGTTCTATGAGTAGCCACATGTTT7332221.0332857113585217No Hit
TCATACCGCTCTAGGTCCCAGGAAAGTAGGATCAAGTAGGCACTGCACTC6600480.9301659895785581No Hit
GCTGTTTAGCAGTCTTCACTGTTGATACCTTCAGGTGCTGGATAATCTGA6506830.9169684577438988No Hit
CCAAAAAGGGAAGATTCTAGCAGCAAGGTCCCATTCTGACTCCAAGATGT6475670.9125772661585492No Hit
AAATGTCACACTAACTGGTGAATGTTTGAGATGGAGAACTGTTGGAGGAG4916250.6928175748226775No Hit
AAAACAATGGAGAAAGAAAAAAAAAGATTGTGTTTTTAGAGGATAAATTC4896390.6900188243449803No Hit
ACTCCTAAACTGTAAAGTTGCTGTTAGTTAGACCACCCCTGGCCCAAAGC3403300.47960662138703647No Hit
GCTGTTTAGCAGTCTTCACTGTTGATACCTCCAGGTACTGGAAAATCCAA734170.10346216708010478No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)